A5025525591- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Enzyme Structure and Function
- Biopolymer Synthesis and Applications
- DNA and Nucleic Acid Chemistry
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- DNA Repair Mechanisms
- Amino Acid Enzymes and Metabolism
- Signaling Pathways in Disease
- Cancer Research and Treatments
- Nanoplatforms for cancer theranostics
- Transgenic Plants and Applications
- Biochemical and Molecular Research
- CRISPR and Genetic Engineering
- Cell Adhesion Molecules Research
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
Hebei University
2023-2025
ShanghaiTech University
2023-2024
University of Maryland, Baltimore
2005-2024
University of Maryland, College Park
2012
Acquired drug resistance to mycotic infections is rapidly emerging as a major medical problem.Opportunistic fungal create therapeutic challenges, particularly in high risk immunocompromised patients with AIDS, cancer, and those undergoing transplantation.Higher mortality and/or morbidity rates due invasive mycosis have been increasing over the last 20 years, light of growing commonly used antibiotics, novel antifungal drugs approaches are required.Currently there considerable interest...
Abstract In plants, the rapid accumulation of proline is a common response to combat abiotic stress 1–7 . Delta-1-pyrroline-5-carboxylate synthase (P5CS) rate-limiting enzyme in synthesis, catalysing initial two-step conversion from glutamate 8 Here we determine first structure plant P5CS. Our results show that Arabidopsis thaliana P5CS1 (AtP5CS1) and P5CS2 (AtP5CS2) can form enzymatic filaments substrate-sensitive manner. The destruction AtP5CS by mutagenesis leads significant reduction...
Successful gene therapy depends on the development of efficient, non-toxic delivery systems. To accomplish this objective, our laboratory has focused solid-phase synthesized peptide carriers, in which amino acid sequence can be varied precisely to augment intracellular DNA transport. We previously determined that linear and branched co-polymers histidine lysine combination with liposomes enhanced efficiency transfection. In study, we have modified two histidine-lysine (HK) peptides by adding...
Sequence-specific gene silencing with small interfering RNA (siRNA) has transformed basic science research, and the efficacy of siRNA therapeutics toward a variety diseases is now being evaluated in pre-clinical clinical trials.Despite its potential value, highly negatively charged classic delivery problem requiring transport across cell membranes to cytosol.Consequently, carrier development for one most important problems solve before can achieve widespread use.An assortment non-viral...
The elevated levels of lactate in tumor tissue play a pivotal role fostering an immunosuppressive microenvironment. Therefore, efficiently reducing to reprogram immune microenvironment (TIM) is considered crucial step for boosted immunotherapy. Here, high-lactate-metabolizing photosynthetic bacteria (LAB-1) selectively screened TIM reprogramming, which then improves the efficacy culture medium LAB-1 screening initially developed through orthogonal experiment, simulating (TME) and utilizing...
Genome editing with the use of zinc finger nucleases has been successfully applied to variety a eukaryotic cells. Furthermore, proof concept for this approach extended diverse animal models from
Glutamate kinase (GK) is the first committed enzyme in proline biosynthesis pathway. Belonging to amino acid (AAK) superfamily, most prokaryotic GKs have an additional PseudoUridine synthase and Archaeosine transglycosylase (PUA) domain at C-terminus, while function of PUA GK poorly understood. Here, we find that Escherichia coli (EcGK) assembles into filaments bundles state apo binding. Using cryogenic electron microscopy, determine high-resolution structures EcGK bundles. The necessary for...
Abstract In plants, the rapid accumulation of proline is a common response to combat abiotic stress. Delta-1-pyrroline-5-carboxylate synthase (P5CS) rate-limiting enzyme in synthesis, catalyzing initial two-step conversion from glutamate proline. Here, we determine first structure plant P5CS. Our results show that Arabidopsis thaliana P5CS2 (atP5CS2) can form enzymatic filaments substrate-sensitive manner. The destruction atP5CS by mutagenesis leads significant reduction activity....