A5085188130- Antimicrobial Peptides and Activities
- Cerebrovascular and genetic disorders
- Biochemical and Structural Characterization
- Bacteriophages and microbial interactions
- Chemical Synthesis and Analysis
- Probiotics and Fermented Foods
- Protein Hydrolysis and Bioactive Peptides
- Immune Response and Inflammation
- Microbial Natural Products and Biosynthesis
- Bacterial biofilms and quorum sensing
- Antimicrobial agents and applications
- Antifungal resistance and susceptibility
- Polydiacetylene-based materials and applications
- Gut microbiota and health
- Supramolecular Self-Assembly in Materials
- Ruminant Nutrition and Digestive Physiology
- Neurological diseases and metabolism
- Transgenic Plants and Applications
- vaccines and immunoinformatics approaches
- Peptidase Inhibition and Analysis
- Insect and Pesticide Research
- Nitrogen and Sulfur Effects on Brassica
- Fungal Biology and Applications
- Neurological Disease Mechanisms and Treatments
- Lipid Membrane Structure and Behavior
Sun Yat-sen University
2022-2024
The Seventh Affiliated Hospital of Sun Yat-sen University
2024
Northeast Agricultural University
2014-2022
Institute of Infection and Immunity
2022
Harbin University
2019
University of Maryland, Baltimore
2010
Acquired drug resistance to mycotic infections is rapidly emerging as a major medical problem.Opportunistic fungal create therapeutic challenges, particularly in high risk immunocompromised patients with AIDS, cancer, and those undergoing transplantation.Higher mortality and/or morbidity rates due invasive mycosis have been increasing over the last 20 years, light of growing commonly used antibiotics, novel antifungal drugs approaches are required.Currently there considerable interest...
Antimicrobial peptides are an important weapon against invading pathogens and potential candidates as novel antibacterial agents, but their antifungal activities not fully developed. In this study, a set of imperfectly amphipathic was developed based on the palindromic structure R n(XRXXXRX)R n ( = 1, 2; X represents L, I, F, or W), engineered exhibited high antimicrobial all fungi bacteria tested (including fluconazole-resistant Candida albicans), with geometric mean (GM) MICs ranging from...
Currently, the majority of antibiotics in clinical use have broad activity spectra, killing pathogenic and beneficial microorganisms indiscriminately. The disruption ecological balance normal flora often results secondary infections or other antibiotic-associated complications. Therefore, targeted antimicrobial therapies capable specifically eliminating bacteria while retaining protective benefits a microflora would be advantageous. In this study, we successfully constructed series...
The emergence of multidrug-resistant bacterial pathogens is a growing threat to global public health. Here, we report the development and characterization panel nine–amino acid residue synthetic peptides that display potent antibacterial activity ability disrupt preestablished microbial biofilms. lead peptide (Peptide K6) showed bactericidal against Pseudomonas aeruginosa Staphylococcus aureus in culture monocultures mixed biofilms vitro. Biophysical analysis revealed Peptide K6...
Abstract We used a template-assisted approach to develop synthetic antimicrobial peptides, which differ from naturally occurring peptides that can compromise host natural defenses. Previous researches have demonstrated symmetrical distribution patterns of amino acids contribute the activity peptides. However, there is little research describing such design ideas for α-helical Therefore, here, we established centrosymmetric sequence template (y + hhh y) n (h, hydrophobic acid; +, cationic y,...
Broad-spectrum antibiotics have, until now, been the mainstay of antibiotic therapy. However, increasing threat drug-resistant bacteria and ecological imbalance normal microbial communities have forced a reconsideration best strategies to treat such pathogens. Therefore, antibacterial agents with specific abilities eliminating pathogens may provide long-term protection. Antimicrobial peptides (AMPs), which can be optimized by modifying their primary sequences, are regarded as potentially...
The unusual acidic pH of the abscess milieu is an adverse factor that decreases therapeutic efficacy traditional antibiotics. Moreover, avoiding both undesired killing commensal bacteria and development drug resistance remains difficult during therapy. Hence, we synthesized a series pH-responsive antimicrobial peptides equipped with efficient bacterial activity at 6.5 inactivity 7.4. Among peptides, F5 exhibited outstanding low toxicity. Fluorescence spectroscopy electron microscopy...
Antimicrobial peptides (AMPs) are excellent candidates to combat the increasing number of multi- or pan-resistant pathogens worldwide based on their mechanism action, which is different from that antibiotics. In this study, we designed short by fusing an α-helix and β-turn sequence-motif in a symmetric-end template promote higher cell selectivity, antibacterial activity salt-resistance these structures. The results showed PQ PP tended form α-helical structure upon interacting with...
The significance of the complex bacterial ecosystem in human body and impediment mammalian membrane against many antibiotics together emphasize necessity to develop antimicrobial agents with precise cell-penetrating activities. A simple feasible method for generating dual-function peptides inspired by highly hydrophobic peptide pheromone cationic is presented. Furthermore, extension candidate library achieved modifying charged domain. bacteria-selective L1, L2, L10, L11 kill Streptococcus...
Abstract Background Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a cerebral small vessel disease that carries mutations in NOTCH3 . The clinical manifestations are influenced by genetic environmental factors may include gut microbiome. Results We investigated the fecal metagenome, metabolome, serum neurotransmitters, cytokines cohort of 24 CADASIL patients 28 healthy household controls. integrated-omics study showed harbored an...
ABSTRACT The antipseudomonal efficiency and mechanism of action a novel engineered antimicrobial peptide, T9W, were evaluated in this study. T9W displayed high activity, with lethal concentration (LC) 1 to 4 μM against Pseudomonas aeruginosa , including ciprofloxacin-, gentamicin-, ceftazidime-resistant strains, even the presence 50 300 mM NaCl, 5 Ca 2+ or 0.5 2 Mg . time-kill curve (TKC) analysis demonstrated concentration-dependent achieving complete killing less than 30 min at 1× LC 4×...
Here, we found that simple substitution of amino acids in the middle position hydrophobic face an amphipathic peptide RI16 with tryptophan (T9W) considerably transformed into antimicrobial specifically targeting Pseudomonas aeruginosa. Minimal inhibitory concentration (MIC) results demonstrated T9W had a strong and activity against P. aeruginosa, including antibiotic-resistant strains, but was not active Escherichia coli, Salmonella typhimurium, Staphylococcus aureus Staphyfococcus...
Broad-spectrum antimicrobial peptides (AMPs) kill bacteria indiscriminately, increasing the possibility of an ecological imbalance in microbiota. To solve this problem, new types AMPs, which pathogenic without breaking micro-ecological balance body, were proposed. Here, we successfully designed a targeting AMP, S2, is fusion peptide composed species-specific domain and broad-spectrum AMP domain. In current study, S2 showed specific killing activity against Staphylococcus aureus, almost no...
Development of probiotic-ineffective antimicrobial peptides (AMPs)-based coatings that can kill pathogenic bacteria at low concentrations but are essentially harmless (even high concentrations) to probiotic organisms is a relatively new trend for therapy against GI tract infections. In this study, series triplet-tryptophan-pivot with various hydrophilic amino acids was constructed. One AMP in particular, S7, showed bactericidal activity Staphylococcus epidermidis, Pseudomonas aeruginosa,...
High manufacturing costs and weak cell selectivity have limited the clinical application of naturally occurring peptides when faced with an outbreak drug resistance. To overcome these limitations, a set antimicrobial was synthesized general sequence (WL) n , where = 1, 2, 3, WL truncated from N‐terminus Cecropin P1 without initial serine residues. The peptide WL3 exhibited stronger activity against both Gram‐negative Gram‐positive microbes than parental CP‐1. showed no hemolysis even at...
Candida albicans, an opportunistic fungus, causes dental caries and contributes to mucosal bacterial dysbiosis leading a second infection. Furthermore, C.albicans forms biofilms that are resistant medicinal treatment. To make matters worse, antifungal resistance has spread (albeit slowly) in this species. Thus, it been imperative develop novel, drug compounds. Herein, peptide was engineered with the sequence of RRFSFWFSFRR-NH2; named P19. This novel observed exert disruptive effects on...
Antibiotic resistance is emerging as a hot issue with the abuse and overuse of antibiotics, shortage effective antimicrobial agents against multidrug resistant bacteria creates huge problem to treat threatening nosocomial skin soft tissue infection. Antimicrobial peptides (AMPs) exhibite enormous potential one most promising candidates antibiotic fight pathogenic infections because its unique membrane penetration mechanism kill pathogens, whereas clinical application AMPs still faces...
ABSTRACT Antibiotic resistance poses a significant public health threat worldwide. The rise in antibiotic and the sharp decline effective antibiotics necessitate development of innovative antibacterial agents. Based on central symmetric structure glycine–serine–glycine, combined with tryptophan arginine, we designed range antimicrobial peptides (AMPs) that exhibited broad-spectrum activity. Notably, AMP W 5 demonstrated rapid sterilization against methicillin-resistant Staphylococcus aureus...