A5073061575- Cerebrovascular and genetic disorders
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Menstrual Health and Disorders
- Erythrocyte Function and Pathophysiology
- Inflammasome and immune disorders
- Diet and metabolism studies
- Neurological Disease Mechanisms and Treatments
- Cancer Immunotherapy and Biomarkers
- Metabolomics and Mass Spectrometry Studies
- Pharmacological Effects of Natural Compounds
- Escherichia coli research studies
- Biochemical effects in animals
- Enhanced Oil Recovery Techniques
- Pancreatic and Hepatic Oncology Research
- Streptococcal Infections and Treatments
- Cancer Research and Treatments
- Antimicrobial Peptides and Activities
- Mycobacterium research and diagnosis
- Hydraulic Fracturing and Reservoir Analysis
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
Sixth Affiliated Hospital of Sun Yat-sen University
2020-2024
Sun Yat-sen University
2020-2024
Shandong University of Traditional Chinese Medicine
2022-2024
China University of Petroleum, Beijing
2023-2024
Massachusetts General Hospital
2013-2023
Harvard University
2013-2023
Boston VA Research Institute
2022-2023
Institute of Infection and Immunity
2022
South China Agricultural University
2022
Broad Institute
2018
SARS-CoV-2, the causative agent for COVID-19, infect human mainly via respiratory tract, which is heavily inhabited by local microbiota. However, interaction between SARS-CoV-2 and nasopharyngeal microbiota, association with metabolome has not been well characterized. Here, metabolomic analysis of blood, urine, swabs from a group COVID-19 non-COVID-19 patients, metagenomic pharyngeal samples were used to identify key features COVID-19. Results showed lactic acid, l-proline, chlorogenic acid...
The emergence of multidrug-resistant bacterial pathogens is a growing threat to global public health. Here, we report the development and characterization panel nine–amino acid residue synthetic peptides that display potent antibacterial activity ability disrupt preestablished microbial biofilms. lead peptide (Peptide K6) showed bactericidal against Pseudomonas aeruginosa Staphylococcus aureus in culture monocultures mixed biofilms vitro. Biophysical analysis revealed Peptide K6...
Pyroptosis is an inflammatory form of cell death induced upon recognition invading microbes. During infection, pyroptosis enhanced in interferon-gamma-exposed cells via the actions members guanylate-binding protein (GBP) family. GBPs promote caspase-4 (CASP4) activation by enhancing its interactions with lipopolysaccharide (LPS), a component outer envelope Gram-negative bacteria. Once activated, CASP4 promotes formation noncanonical inflammasomes, signaling platforms that mediate pyroptosis....
Gut microbiota of centenarians has garnered significant attention in recent years, with most studies concentrating on the analysis microbial composition. However, there is still limited knowledge regarding consistent signatures specific species and their biological functions, as well potential causal relationship between gut longevity. To address this, we performed fecal metagenomic eight longevous populations at functional level, employed Mendelian randomization (MR) to infer associations...
Abstract Background Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a cerebral small vessel disease that carries mutations in NOTCH3 . The clinical manifestations are influenced by genetic environmental factors may include gut microbiome. Results We investigated the fecal metagenome, metabolome, serum neurotransmitters, cytokines cohort of 24 CADASIL patients 28 healthy household controls. integrated-omics study showed harbored an...
Significance The virulence of numerous bacterial pathogens is dependent on nanomachines that inject tens proteins (effectors) into host cells. Effectors are often functionally redundant or act in an additive manner to usurp cellular processes, limiting their characterization via traditional top-down loss-of-function assays. To overcome this issue, we developed innovative synthetic bottom-up platform conduct gain-of-function screens. These screens identified previously missed Shigella...
ABSTRACT Hemolysins produced by Vibrio anguillarum have been implicated in the development of hemorrhagic septicemia during vibriosis, a fatal fish disease. Previously, two hemolysin gene clusters responsible for hemolysis and cytotoxicity V. were identified: vah1-plp cluster rtxACHBDE cluster. In this study, we identified hns gene, which encodes H-NS protein acts as negative regulator both clusters. The shares strong homology with other bacterial proteins. An mutant exhibited increased...
Abstract Background Vibrio anguillarum is the causative agent of vibriosis in fish. Several extracellular proteins secreted by V. have been shown to contribute virulence. While two hemolysin gene clusters, vah1-plp and rtxACHBDE , previously identified described, activities protein encoded plp were not known. Here we describe biochemical -encoded its role pathogenesis. Results The gene, one components vah1 cluster, encodes a 416-amino-acid (Plp), which has homology lipolytic enzymes...
ABSTRACT The two hemolysin gene clusters previously identified in Vibrio anguillarum , the vah1 cluster and rtxACHBDE cluster, are responsible for hemolytic cytotoxic activities of V. fish. In this study, we used degenerate PCR to identify a positive regulatory gene, hlyU from unsequenced genome. encodes 92-amino-acid protein is highly homologous other bacterial HlyU proteins. An mutant was constructed, which exhibited an ∼5-fold decrease activity on sheep blood agar with no statistically...
The aim of this study was to investigate the responses non-starch polysaccharide (NSP) enzymes and protease combination on growth performance, meat quality, nutrients digestibility yellow-feathered broilers fed with corn-soybean meal basal diets normal subnormal crude protein (CP) levels. experimental design completely randomized a 2 × factorial arrangement treatments, including six replicates 20 birds per pen. Two were formulated CP level as positive control (PC) without extra essential...
Surfactant huff-n-puff (HnP) has been shown to be an effective protocol improve oil recovery in tight and ultratight reservoirs. The success of surfactant HnP for enhanced (EOR) process depends on the efficiency designed chemical formula, as formation situ microemulsion by injection is considered most desirable condition achieving ultra-low interfacial tension during process. In this work, we conducted experimental studies mechanism EOR Mahu reservoir. Salinity scan experiments were carried...
Peptostreptococcus anaerobius is an anaerobic bacterium, which has been found selectively en-riched in the fecal and mucosal microbiota of colorectal cancer (CRC) patients. Emerging evidence suggest P. may contribute to development CRC human. In this study, we designed a multi-epitope chimeric vaccine against PCWBR2, recently identified adhesin that interacts directly with colon cell lines by binding α2/β1 integrin frequently overexpressed human tumors lines. Immunoinformatics tools...
Vibrio anguillarum is an extracellular bacterial pathogen that a causative agent of vibriosis in finfish and crustaceans with mortality rates ranging from 30% to 100%. Mutations central metabolism (glycolysis the TCA cycle) intracellular pathogens often result attenuated virulence due depletion required metabolic intermediates; however, it was not known whether mutations would affect such as V. anguillarum. Seven mutants were created characterized regard growth minimal complex media,...
A plethora of studies have reported the associations between microbiota and multiple diseases, leading to development at least four databases demonstrate microbiota-disease associations, i.e., gutMDisorder, mBodyMap, Gmrepo, Amadis. Moreover, gut mediates drug efficacy toxicity, whereas a comprehensive database elucidate microbiota-drug is lacking. Here, we report an open-access knowledge base, MADET (Microbiomics Anticancer Drug Efficacy Toxicity), which harbors 483 manually annotated from...
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Introduction Chronic kidney disease (CKD) is worldwide healthcare burden with growing incidence and death rate. Emerging evidence demonstrated the compositional functional differences of gut microbiota in patients CKD. As such, microbial features can be developed as diagnostic biomarkers potential therapeutic target for Methods To eliminate outcome bias arising from factors such geographical distribution, sequencing platform, data analysis techniques, we conducted a comprehensive between CKD...