A5005326970- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Hepatitis C virus research
- Drug-Induced Hepatotoxicity and Protection
- Liver physiology and pathology
- Liver Disease and Transplantation
- Immune Cell Function and Interaction
- Poisoning and overdose treatments
- Long-Term Effects of COVID-19
- SARS-CoV-2 detection and testing
- MicroRNA in disease regulation
- Organ Transplantation Techniques and Outcomes
- Liver Diseases and Immunity
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Mesenchymal stem cell research
- COVID-19 epidemiological studies
- Gut microbiota and health
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- COVID-19 diagnosis using AI
- Plant-based Medicinal Research
- Immunotherapy and Immune Responses
Third Affiliated Hospital of Sun Yat-sen University
2013-2025
Sun Yat-sen University
2013-2025
Foundation for Human Potential
2017
Northwestern University
2013
Vall d'Hebron Hospital Universitari
2011
Abstract Purpose The purpose of this study was to detect severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) ribonucleic acid (RNA) in urine and blood specimens, anal oropharyngeal swabs from patients with confirmed SARS‐CoV‐2 infection, correlated positive results clinical findings. Methods Patients infections were included study. Patients' demographic data recorded. Quantitative real‐time polymerase chain reaction used RNA swabs. is registered at ClinicalTrials.gov (No....
Our study aimed to investigate the short-term efficacy and long-term prognosis of liver failure patients caused by hepatitis B after a single transplantation with autologous marrow mesenchymal stem cells (MMSCs). A total 527 inpatients were recruited received same medical treatments, among whom 53 underwent MMSCs. 105 matched for age, sex, biochemical indexes, including alanine aminotransferase (ALT), albumin, bilirubin (TBIL), prothrombin time (PT), Model End-Stage Liver Disease (MELD),...
Mortality from hepatitis B virus (HBV)–related acute‐on‐chronic liver failure (ACLF) is high due to limited treatment options. Preclinical and clinical investigations have proved that with mesenchymal stromal cells (MSCs) beneficial for recovery injury. We hypothesized the outcome of HBV‐related ACLF would be improved by MSC treatment. From 2010 2013, 110 patients were enrolled in this open‐label, nonblinded randomized controlled study. The control group (n = 54) was treated standard medical...
Abstract Background The role of circRNAs in hepatocellular carcinoma (HCC) progression remains unclear. CircPIAS1 (circBase ID: hsa_circ_0007088) was identified as overexpressed HCC cases through bioinformatics analysis. This study aimed to investigate the oncogenic properties and mechanisms circPIAS1 development. Methods Functional analyses were conducted assess circPIAS1’s impact on cell proliferation, migration, ferroptosis. Xenograft mouse models employed evaluate effects tumor growth...
Abstract We tested samples collected from nine patients diagnosed with coronavirus disease 2019 (COVID-19). The virus was found in urine, blood, anal swabs and oropharyngeal swabs. It is the first time for SARS-CoV-2 though no urinary irritation found.
SARS-CoV-2, the causative agent for COVID-19, infect human mainly via respiratory tract, which is heavily inhabited by local microbiota. However, interaction between SARS-CoV-2 and nasopharyngeal microbiota, association with metabolome has not been well characterized. Here, metabolomic analysis of blood, urine, swabs from a group COVID-19 non-COVID-19 patients, metagenomic pharyngeal samples were used to identify key features COVID-19. Results showed lactic acid, l-proline, chlorogenic acid...
Primary biliary cirrhosis (PBC) is an autoimmune liver disease characterized by the presence of anti-mitocondrial autoantibodies (AMA) which has essential role also for diagnosis. In addition, some anti-nuclear antibodies (ANA) have been shown to be highly specific PBC. The purpose this study was assess prevalence PBC among adults referring hospital annual health check-up in Southern China screening sera PBC-specific autoantibodies.AMA and ANA were screened 8,126 (mean age 44 ± 15 years, 48%...
Background and aim Bile acid (BA) synthesis in the liver is regulated by Fibroblast Growth Factor 19 (FGF19) secreted from ileum as an enterohepatic feedback mechanism. Although FGF19 mRNA absent normal liver, gene expression was reported to increase response both extrahepatic intrahepatic cholestasis. The impact of upregulated on BA unclear overall role circulating under cholestatic conditions needs be further investigated. Methods directly quantified measuring serum concentrations...
Abstract Acute-on-chronic liver failure is a distinct clinical syndrome characterized by dysregulated immune response and extensive hepatocyte death without satisfactory therapies. As cytoplasmic degradative quality-control process, autophagy was implicated in maintaining intracellular homeostasis, decreased hepatic found many diseases contributes to disease pathogenesis. Previously, we identified the therapeutic potential of mesenchymal stem cells (MSCs) ACLF patients; however, intrinsic...
Acute-on-chronic liver failure (ACLF) is a severe disease with high mortality. Macrophage-related inflammation plays crucial role in ACLF development. Mesenchymal stem cells (MSCs) treatment was demonstrated to be beneficial our previous study; however, the underlying mechanisms remain unknown. Therefore, mouse bone marrow-derived MSCs were used treat an model or cocultured RAW264.7/J774A.1 macrophages that stimulated LPS. Histological and serological parameters survival analyzed evaluate...
Balancing hepatocyte death and proliferation is key to non-transplantation treatments for acute liver failure (ALF), which has a high short-term mortality rate. Small extracellular vesicles (sEVs) may act as mediators in the repair of damaged tissue by mesenchymal stem cells (MSCs). We aimed investigate efficacy human bone marrow MSC-derived sEVs (BMSC-sEVs) treating mice with ALF molecular mechanisms involved regulating apoptosis. EVs sEV-free BMSC concentrated medium were injected into...
It is unclear whether nucleos(t)ide analogs (NUCs) continuation provides clinical benefits following HBsAg seroclearance with pegylated interferon (PEG-IFN)-based therapy. This study aims to investigate the role of NUCs in seroreversion. Patients who experienced serum loss after PEG-IFN-based therapy were enrolled and followed up for 96 weeks. Propensity score matching (PSM) was performed using a 1:1 ratio adjust associated factors. A multivariate logistic regression analysis used determine...
The gut microbiota in the hepatitis B virus related acute-on-chronic liver failure (HBV-ACLF) is poorly defined. We aim to uncover characteristics of HBV-ACLF and other HBV associated pathologies. analyzed microbiome patients with or pathologies healthy individuals by 16S rRNA sequencing metagenomic fecal samples. 212 HBV-ACLF, 252 chronic (CHB), 162 HBV-associated cirrhosis (HBV-LC) 877 were recruited for study. CHB HBV-LC are grouped as HBV-Other. discovered striking differences diversity...
Acute-on-chronic liver failure (ACLF) is a severe syndrome associated with high mortality. Alterations in the microenvironment are one of vital causes immune damage and dysfunction. Human bone marrow mesenchymal stem cells (hBMSCs) have been reported to alleviate injury via exosome-mediated signaling; note, miRNAs most important cargoes exosomes. Importantly, within exosomes hepatic may mediate cell (MSC)-derived regulation function. This study investigated hepatocyte exosomal which...
Abstract The immune mechanism underlying hepatitis B surface antigen (HBsAg) loss, particularly type I inflammatory response, during pegylated interferon‐α (PEG‐IFN) therapy remains unclear. In this study, we aimed to elucidate such mechanisms. Overall, 82 patients with chronic (CHB), including 41 HBsAg loss (cured group) and uncured patients, received nucleos(t)ide analogue PEG‐IFN treatments. Blood samples from all liver tissues 14 CHB, hepatic perfusate 8 donors were collected for...
BackgroundOlgotrelvir is an oral antiviral with dual mechanisms of action targeting severe acute respiratory syndrome coronavirus 2 main protease (i.e., Mpro) and human cathepsin L. It has potential to serve as a single-agent treatment disease 2019 (Covid-19).MethodsWe conducted phase 3, double-blind, randomized, placebo-controlled trial evaluate the efficacy safety olgotrelvir in 1212 nonhospitalized adult participants mild moderate Covid-19, irrespective risk factors, who were randomly...
Mediator, a large multisubunit protein complex, plays pivotal role in gene transcription by linking gene-specific factors with the preinitiation complex and RNA polymerase II. In liver, key subunit of Mediator Med1, interacts several nuclear receptors to direct transcription. Conditional knock-out Med1 liver showed that hepatocytes lacking did not regenerate following either partial hepatectomy or treatment certain receptor activators failed give rise tumors when challenged carcinogens. We...