Abstract Antimicrobial peptides (AMPs) have recently attracted a great deal of attention as promising antibiotic candidates, but some obstacles such toxicity and high synthesis cost must be addressed before developing them further. For short with improved cell selectivity, we designed series modified PMAP-36 analogues. assays showed that decreasing chain length in certain range retained the antimicrobial activity parental peptide reduced hemolysis. The 18-mer RI18 exhibited excellent against...

Abstract Early weaning usually causes intestinal disorders, enteritis, and diarrhea in young animals human infants. Astragalus polysaccharides (APS) possesses anti‐inflammatory activity. To study the mechanisms of APS its potential effects on health, we performed an RNA sequencing (RNA‐seq) lipopolysaccharide (LPS)‐stimulated porcine epithelial cells (IPEC‐J2) vitro. In addition, LPS‐stimulated BALB/c mice were used to inflammation vivo. The results from RNA‐seq analysis show that there 107,...

Antimicrobial peptides (AMPs) show potential as antibiotic alternatives for bacterial infections; nevertheless, the susceptibility to proteases limits their broader utilization. This study developed engineered lipopeptides using antienzymolysis modifications and cysteine (Cys)-dimerization strategy. As key parameters functioning of AMPs, hydrophobicity positive charges were concentrated within peptide sequence by adjusting intermolecular disulfide bond placement distribution effects. Their...

The emergence of multidrug-resistant (MDR) pathogens, coupled with the limited effectiveness existing antibiotics in eradicating biofilms, presents a significant threat to global health care. This critical situation underscores urgent need for discovery and development antimicrobial agents. Recently, peptide-derived nanomaterials have shown promise combating such infections. Amino acid noncovalent forces, notably π–π stacking electrostatic interactions, remain underutilized guiding...

Intracellular bacterial infections and antimicrobial resistance are threatening global public health systems. Antimicrobial peptides a potential solution to combat resistance, but the design of self-assembled nanopeptides with dual functions cell penetration antibacterial properties intracellular bacteria remains challenge. Here, we propose strategy develop through chimerization core, hydrophobic motif, cell-permeable unit. The optimal nanopeptides, F3FT N3FT, exhibited potent activity...

Antimicrobial peptides (AMPs), which present in the non-specific immune system of organism, are amongst most promising candidates for development novel antimicrobials. The modification naturally occurring AMPs based on their residue composition and distribution is a simple effective strategy optimization known AMPs. In this study, series truncated residue-substituted derivatives antimicrobial peptide PMAP-36 were designed synthesized. 24-residue peptide, GI24, displayed activity comparable...

Poor proteolytic resistance is an urgent problem to be solved in the clinical application of antimicrobial peptides (AMPs), yet common solutions, such as complicated chemical modifications and utilization d-amino acids, greatly increase difficulty cost producing AMPs. In this work, a set novel was synthesized based on antitrypsin/antichymotrypsin hydrolytic peptide structure unit (XYPX)n (X represents I, L, V; Y R K), which designed using systematic natural amino acid arrangement. Of these...

Resveratrol, a plant phenol, affords protection against inflammation and oxidative stress. The objective of this study was to investigate the effects dietary resveratrol supplementation during pregnancy lactation on antioxidant status sows piglets gene expression pathway in placenta.Forty were allotted 2 treatments 20 d after breeding. Sows fed control diet with 300 mg/kg resveratrol. Oxidative stress biomarkers enzymes measured placenta, milk, plasma piglets. Antioxidant protein Kelch-like...

ABSTRACT Antimicrobial peptides with amphipathic β-hairpin-like structures have potent antimicrobial properties and low cytotoxicity. The effect of VR or RV motifs on has not been investigated. In this study, a series peptides, Ac-C(VR) n D PG (RV) C-NH 2 ( = 1, 2, 3, 4, 5), were synthesized, the chain length activity was evaluated. initially increased then decreased length. Longer stimulated toxicity to mammalian cells. VR3, 16-mer peptide seven amino acids in strand, displayed highest...

The present study was conducted to investigate the effects of maternal zearalenone (ZEN) exposure on intestine pregnant Sprague-Dawley (SD) rats and its offspring. Ninety-six SD were randomly divided into four groups fed with diets containing ZEN at concentrations 0.3 mg/kg, 48.5 97.6 mg/kg or 146.0 from gestation days (GD) 1 7. All mycotoxin-free diet until their offspring weaned three weeks age. small intestinal fragments GD8, dams pups collected studied for toxic antioxidant status,...

Self-assembling nanometer-scale structured peptide polymers and dendrimers have shown promise in biomedical applications due to their versatile properties easy availability. Herein, self-assembling dendron nanoparticles (SPDNs) with potent antimicrobial activity against a range of bacteria were developed based on the nanoscale self-assembly an arginine–proline repeat branched bearing hexadecanoic acid chain. The SPDNs are biocompatible, our most active nanoparticle, C16-3RP, was found...

Antimicrobial peptides are an important weapon against invading pathogens and potential candidates as novel antibacterial agents, but their antifungal activities not fully developed. In this study, a set of imperfectly amphipathic was developed based on the palindromic structure R n(XRXXXRX)R n ( = 1, 2; X represents L, I, F, or W), engineered exhibited high antimicrobial all fungi bacteria tested (including fluconazole-resistant Candida albicans), with geometric mean (GM) MICs ranging from...

Inflammatory bowel disease (IBD) develops as a result of complicated interactions between genetic susceptibility, excessive innate immunity, and environmental factors, which are mainly related to the gut microbiota. The present study aimed elucidate protective effects underlying mechanisms short-chain fatty acid salt, sodium butyrate, on colonic inflammation induced by dextran sulfate (DSS) in mice. Pretreatment with butyrate attenuated colitis, demonstrated decreased activity index (DAI),...