A5017390096- Porphyrin Metabolism and Disorders
- Salivary Gland Disorders and Functions
- Gut microbiota and health
- Peptidase Inhibition and Analysis
- Neonatal Health and Biochemistry
- Radiopharmaceutical Chemistry and Applications
- Folate and B Vitamins Research
- S100 Proteins and Annexins
- Bacterial Genetics and Biotechnology
- Oral microbiology and periodontitis research
- Enzyme Structure and Function
- Genomics and Phylogenetic Studies
- Antibiotics Pharmacokinetics and Efficacy
- RNA and protein synthesis mechanisms
- Hemoglobin structure and function
- Bacteriophages and microbial interactions
- Neurological diseases and metabolism
- Gene expression and cancer classification
- Clostridium difficile and Clostridium perfringens research
- Pediatric health and respiratory diseases
- Antibiotic Resistance in Bacteria
- Oral health in cancer treatment
- Microbial Natural Products and Biosynthesis
- Dental Trauma and Treatments
- Biochemical and Molecular Research
Université Paris-Saclay
2023-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2023-2025
Research Network (United States)
2023-2024
Laboratoire d'Analyses Génétiques pour les Espèces Animales
2023
Smiths Detection (France)
1992-1994
Summary A 4.6 kb Staphylococcus aureus DNA fragment containing gyrase‐like genes ( grlA and grlB ) was cloned sequenced. The proteins GrlA GrlB exhibit more than 30% identity with E. coli topoisomerase IV sub units the gyrase subunits from S. Escherichia coli. combined cell extracts of overproducing strains catalysed ATP‐dependent relaxation decatenation specific to IV. temperature‐sensitive phenotype Salmonella typhimurium parC parE mutants complemented by genes, when two were co‐expressed....
Metagenomic sequencing provides profound insights into microbial communities, but it is often compromised by technical biases, including cross-sample contamination. This underexplored phenomenon arises when content inadvertently exchanged among concurrently processed samples. Such contamination that distort profiles, poses significant risks to the reliability of metagenomic data and downstream analyses. Despite its critical impact, this issue remains insufficiently addressed. To fill gap, we...
Two genes involved in the biosynthesis of depsipeptide antibiotics pristinamycins I (PI) produced by Streptomyces pristinaespiralis were cloned and sequenced. The 1.7-kb snbA gene encodes a 3-hydroxypicolinic acid:AMP ligase, 7.7-kb snbC PI synthetase 2, responsible for incorporating L-threonine L-aminobutyric acid macrocycle. snbC, which encode two first structural enzymes synthesis, are not contiguous. Both located PI-specific transcriptional units, as disruption one or other led to...
Tn5 Sp(r) transposons have been inserted into the 8-kb Pseudomonas denitrificans DNA fragment from complementation group D, which carries cob genes. Genetic analysis and nucleotide sequence revealed that only two genes (cobU cobV) were found on this genomic locus. Nicotinate-nucleotide: dimethylbenzimidazole phosphoribosyltransferase (EC 2.4.2.21) was assayed purified to homogeneity a P. strain in cobU cobV amplified. The enzyme identified as gene product basis of identical molecular weights...
The final enzymatic reaction in the conversion of precorrin-6x to hydrogenobyrinic acid by cell-free protein preparations from Pseudomonas denitrificans was shown be inhibited acid. Use made this property prepare last biosynthetic precursor acid, named precorrin-8x. Double-labeling experiments, mass spectrometry, and UV-visible light spectroscopy studies established that precorrin-8x at oxidation level a corrin differed two additional methyl groups (presumably C-5 C-15) decarboxylation...
Precorrin-6x reductase, which catalyzes the NADPH-dependent reduction of precorrin-6x to a dihydro derivative named precorrin-6y, was purified 14,300-fold homogeneity with an 8% yield from extracts recombinant strain Pseudomonas denitrificans. Precorrin-6y identified by fast atom bombardment-mass spectrometry. It converted in high (90%) hydrogenobyrinic acid cell-free protein preparations P. For purification and characterization coupled-enzyme radioenzymatic assay developed precorrin-6y...
A protein catalyzing methylation at C-5 and C-15 decarboxylation of the acetic acid side chain C-12 on precorrin-6y to yield precorrin-8x was purified homogeneity from a recombinant strain Pseudomonas denitrificans. It sequenced N terminus shown be encoded by cobL gene.
The two consecutive activities of the cobalamin biosynthetic pathway that catalyze conversion cobinamide to phosphate (cobinamide kinase) and GDP-cobinamide guanylytransferase) were shown be carried by same protein in Pseudomonas denitrificans. This bifunctional was purified homogeneity high-performance liquid chromatography extracts a recombinant strain this microorganism, sequence first 10 amino acid residues at N terminus determined. Both specific coenzyme forms corrinoid substrates...
Abstract Background Heterozygous variants in GBA1 are the commonest genetic risk factor for Parkinson disease (PD) but penetrance is incomplete. dysfunction can cause gastrointestinal disturbances and microbiome changes preclinical models. Mounting evidence suggests that microbiota-gut-brain axis potentially implicated PD pathogenesis. Whether gut composition influenced by host genetics heterozygosis has never been explored. Objectives To evaluate whether heterozygosity pathogenic L444P...
This protocol describes the procedures for whole DNA extraction, high throughput sequencing, metagenomic read mapping and bioinformatical pre-processing to determine microbial composition functional potential of mice digestive microbiota.
Recent attention has highlighted the importance of oral microbiota in human health and disease, e.g., Parkinson's notably using shotgun metagenomics. One key aspect for efficient metagenomic analysis relies on optimal microbial sampling DNA extraction, generally implementing commercial solutions developed to improve sample collection preservation, provide high quality quantity downstream analysis. As studies are today performed a large number samples, next evolution increase study throughput...
Abstract The importance of oral microbiota has been recently highlighted in human health, such as Parkinson disease, notably using shotgun metagenomics. One key aspect for efficient metagenomic analysis relies on optimal microbial sampling and DNA extraction, generally implementing commercial solutions developed to improve sample collection preservation provide high quality quantity downstream analysis. Because studies are today performed a large number samples, the next evolution increase...
Abstract The importance of oral microbiota has been recently highlighted in human health, such as Parkinson disease, notably using shotgun metagenomics. One key aspect for efficient metagenomic analysis relies on optimal microbial sampling and DNA extraction, generally implementing commercial solutions developed to improve sample collection preservation provide high quality quantity downstream analysis. Because studies are today performed a large number samples, the next evolution increase...