A5007140629- Mesenchymal stem cell research
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Plasma Applications and Diagnostics
- Electrospun Nanofibers in Biomedical Applications
- Burn Injury Management and Outcomes
- Adipose Tissue and Metabolism
- Bone Tissue Engineering Materials
- Periodontal Regeneration and Treatments
- CAR-T cell therapy research
- Knee injuries and reconstruction techniques
- Pluripotent Stem Cells Research
- Cellular Mechanics and Interactions
- Robot Manipulation and Learning
- Muscle Physiology and Disorders
- Clinical Nutrition and Gastroenterology
- Pharmaceutical studies and practices
- 3D Printing in Biomedical Research
- biodegradable polymer synthesis and properties
- CRISPR and Genetic Engineering
- Modular Robots and Swarm Intelligence
- Graphene and Nanomaterials Applications
- Orthopaedic implants and arthroplasty
- Osteoarthritis Treatment and Mechanisms
University of Rostock
2018-2025
University Medical Center
2020
University Hospital and Clinics
2020
Heidelberg University
2020
Heidelberg University
2020
Universitätsmedizin Rostock
2018
Leibniz Institute for Plasma Science and Technology
2014-2015
University of Cologne
2012
University Hospital Cologne
2012
Over the past few decades, new cold plasma sources have been developed that great advantage of operating at atmospheric pressure and temperatures tolerable by biological material. New applications for these emerged, especially in field dermatology. Recently it was demonstrated atmospheric-pressure positively influences healing chronic wounds. The potential lies its capacity to reduce bacterial load wound while same time stimulating skin cells therefore promoting closure. In recent years,...
Background: Platelet-rich plasma (PRP) is widely used in sports medicine. However, neither preparation nor parameters for clinical application, such as concentration, timing, and number of applications, are standardized, making research utilization challenging. Purpose: To investigate the effect varying doses PRP powder terms different concentrations, applications on human chondrocytes a reproducible cell culture model. Study Design: Controlled laboratory study. Methods: A standardized...
Reactive oxygen species (ROS) can irreversibly damage biological molecules, a process known as oxidative stress. Elevated ROS levels are associated with immune cell activation. Sustained system activation affect many different cells in the environment. One type that has been detected almost all tissues of body is mesenchymal stem/stromal (MSC). MSC possess proliferation and differentiation potential, thus facilitating regeneration processes. However, regenerative capacity might be impaired...
Mesenchymal stem/stromal cells (MSC) are capable of progenitor cell fraction renewal or tissue-specific differentiation. These properties maintained during in vitro cultivation, making them an interesting model system for testing biological and pharmacological compounds. Cell cultivation 2D is commonly used to study cellular responses, but the environment does not reflect structural situation most types. Therefore, 3D culture systems have been developed provide a more accurate physiological...
With the development of plasma sources generating nonthermal plasmas at atmospheric pressure applications broaden enormously, especially in medical field. While earlier physical on biological matter focused inactivation microorganisms, latest studies have revealed a modulation eukaryotic cells by same type plasma. The aim this study was to elucidate molecular mechanisms triggered non-thermal human oral mucosa. Ex vivo biopsies were treated directly with cold jet kinpenMED and analyzed for...
Abstract Background Cancer vaccines provide a complex source of neoantigens. Still, increasing evidence reveals that the neoantigen quality rather than quantity is predictive for treatment outcome. Methods Using preclinical Mlh1 −/− tumor model, we performed side-by side comparison two autologous cell-line derived lysates (namely 328 and A7450 T1 M1) harboring different mutational burden (TMB; i.e. ultra-high: 328; moderate-high: M1). Mice received repetitive prophylactic or therapeutic...
Cold atmospheric pressure plasma (CAP) offers a variety of therapeutic possibilities and induces the formation reactive chemical species associated with oxidative stress. Mesenchymal stem/stromal cells (MSCs) play central role in tissue regeneration, partly because their antioxidant properties ability to migrate into regenerating areas. During application, MSCs are directly exposed CAP. Therefore, investigation CAP-induced effects on is essential. In this study, we quantified amount ROS due...
Multipotent adult mesenchymal stromal cells (MSCs) could represent an elegant source for the generation of patient-specific cardiomyocytes needed regenerative medicine, cardiovascular research, and pharmacological studies. However, differentiation MSC into a cardiac lineage is challenging compared to embryonic stem or induced pluripotent cells. Here we used non-integrative methods, including microRNA mRNA, reprogramming derived from bone marrow, dental follicle, adipose tissue. We found that...
Adipose tissue plays an active role in the regulation of body´s energy balance. Mesenchymal stem/stromal cells from adipose (adMSC) are precursor for repair and adipogenesis. Since balance differentiation state tissue-resident is associated with development various diseases, examination proliferation adMSC might provide new therapeutic targets. Transforming growth factor-β1 (TGF-ß1) synthetized by many cell types involved biological processes. Here, we investigated effects different...
Abstract The animal product most used as a stimulatory additive for cell cultivation is still fetal bovine serum (FBS). Besides the ethical concerns regarding collection, main problems of FBS are batch-to-batch variability and resulting risk lower reproducibility, differences between species, presence undefined/unknown components, contamination. In contrast, pig blood, which by-product slaughter, sufficiently available sustainable resource with high degree standardization in terms donor age,...
Within the healthy human body, cells reside within physiological environment of a tissue compound. Here, they are subject to constant low levels mechanical stress that can influence growth and differentiation cells. The liposuction adipose subsequent isolation mesenchymal stem/stromal (MSCs), for example, procedures induce high level shear stress. As MSCs play central role in regeneration by migrating into regenerating areas driving through proliferation tissue-specific differentiation,...
Abstract Background: Cancer vaccines provide a complex source of neoantigens. Still, increasing evidence reveals that the neoantigen quality rather than quantity is predictive for treatment outcome. Methods: Using preclinical Mlh1 -/- tumor model, we performed side-by side comparison two autologous cell-line derived lysates (namely 328 and A7450 T1 M1) harboring different mutational burden (TMB; i.e. ultra-high: 328; moderate-high: M1). Mice received repetitive prophylactic or therapeutic...
Abstract For the in vitro examination of effects on cells and tissues metal ions derived from implants by wear corrosion, a system is needed to release controlled sustained manner. As small concentrations may have an impact biological systems, corresponding slow over defined period time necessary. Here, manufacturing method polymeric Poly(L-lactic acid) (PLLA) films with incorporated cobalt via dip coating reported. The establishment two analytical methods for detection quantification shown....
Abstract Background: Cancer vaccines provide a complex source of neoantigens. Still, increasing evidence reveals that the neoantigen quality rather than quantity is predictive for treatment outcome. Methods: Using preclinical Mlh1 -/- tumor model, we performed side-by side comparison two autologous cell-line derived lysates (namely 328 and A7450 T1 M1) harboring different mutational burden (TMB; i.e. ultra-high: 328; moderate-high: M1). Mice received repetitive prophylactic or therapeutic...