A5036990174- Plasma Applications and Diagnostics
- Burn Injury Management and Outcomes
- Plasma Diagnostics and Applications
- Wound Healing and Treatments
- Electrohydrodynamics and Fluid Dynamics
- Surface Modification and Superhydrophobicity
- Electrospun Nanofibers in Biomedical Applications
- Dermatologic Treatments and Research
- Heme Oxygenase-1 and Carbon Monoxide
- Metabolism, Diabetes, and Cancer
- Diabetes Treatment and Management
- Microbial Inactivation Methods
- Laser Applications in Dentistry and Medicine
- Aerosol Filtration and Electrostatic Precipitation
- Hemoglobinopathies and Related Disorders
- Cancer, Hypoxia, and Metabolism
- Diabetic Foot Ulcer Assessment and Management
- Pressure Ulcer Prevention and Management
- Cellular Mechanics and Interactions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Peptidase Inhibition and Analysis
- Inhalation and Respiratory Drug Delivery
- Neuropeptides and Animal Physiology
- Chemokine receptors and signaling
- Diabetes Management and Research
Leibniz Institute for Plasma Science and Technology
2015-2024
Center for Innovation
2013-2021
Klinik Bad Oexen
2020
Leibniz Association
2012-2015
Equilibrium Research
2013
Witten/Herdecke University
2001-2012
The mechanism of interaction cold nonequilibrium plasma jets with mammalian cells in physiologic liquid is reported. major biological active species produced by an argon RF jet responsible for cell viability reduction are analyzed experimental results obtained through physical, biological, and chemical diagnostics. This complemented kinetics modeling the source to assess dominant reactive gas phase species. Different chemistries changing feed composition based from argon, humidified (0.27%),...
Plasma medicine is an interdisciplinary field and recent clinical studies showed benefits of topical plasma application to chronic wounds. Whereas most investigations have focused on plasma-skin cell interaction, immune cells are omnipresent in tissues as well. They not only elicit specific responses but also regulate inflammation, which central healing regeneration. generates short-lived radicals species the gas phase. Mechanisms plasma-cell interactions fully understood it hypothesized...
The pathway of the biologically active molecule hydrogen peroxide (H2O2) from plasma generation in gas phase by an atmospheric pressure argon jet, to its transition into liquid and finally inhibiting effect on human skin cells is investigated for different feed humidity settings. Gas diagnostics like Fourier transformed infrared spectroscopy laser induced fluorescence hydroxyl radicals (·OH) are combined with analytics such as chemical assays electron paramagnetic resonance spectroscopy....
Diabetic foot ulcers are a common complication of diabetes and require specialized treatment. Cold atmospheric plasma (CAP) has been associated with benefits in wound infection healing previous smaller series case reports. Yet the effect CAP compared standard care therapy diabetic remains to be studied.To determine whether application accelerates therapy.A prospective, randomized, placebo-controlled, patient-blinded clinical trial was conducted at 2 clinics recruitment from August 17, 2016,...
The development of metastases is a decisive step in the course cancer disease. detection patients correlated with poor prognosis, and over 90% all deaths from are not due to primary tumor, which often can be successfully treated, but metastases. Tumor cell migration, prerequisite for metastasis development, merely genetically determined, distinctly regulated by signal substances environment including chemokines neurotransmitters. We have shown previously that migration breast, prostate,...
In this study, the effect of feed gas humidity on reactive component generation an atmospheric-pressure argon plasma jet and its human skin cells are investigated. Feed is identified as one key parameter that strongly influences stability reproducibility medical studies. The investigated by absorption spectroscopy in ultraviolet infrared spectral region for ozone production depending concentration gas. By optical emission dependence present excited species such hydroxyl radicals, molecular...
Finding a solution for air species contamination of atmospheric pressure plasmas in plasma medical treatment is major task the new field medicine. Several approaches use complex climate chambers to control surrounding atmosphere. In this paper, ambient are excluded plasma-human-skin-cell by ensheathing jet effluent with shielding gas. Not only does gas curtain protect from inflow but it also, more importantly, allows controlling reactive composition adjusting mixture present investigations,...
During the last decade, epidemiological studies uncovered tremendous impact of metabolic syndrome/diabetes mellitus type 2 (DM T2) as risk factors progression cancer. Therefore, we studied diabetogenic glucose and insulin concentrations on activities tumour cells, because little is known about how high levels are influencing gene causing changes in signal cascade with respect to kinases involved proliferation migration cancer cells.To address this question analysed activity more than 400...
Cold atmospheric pressure plasmas represent a favorable option for the treatment of heat sensitive materials and human or animal tissue. Beneficial effects have been documented in variety medical conditions, e.g., chronic wounds. It is assumed that main mechanism plasma’s efficacy mediated by stimulating dissipation energy via radiation and/or chemical energy. Although no evidence on undesired side plasma has yet presented, skepticism toward safety exposure to present. However, only little...
Abstract The use of plasma for healthcare can be dated back as far the middle 19th century. Only development room temperature atmospheric pressure sources in past decade, however, has opened new and fast growing interdisciplinary research field medicine. Three main topics distinguished: treated implants, decontamination, plasmas medical therapy. Understanding processes involved is still incomplete. With aim a more fundamental insight we investigate a) functionalization implants with...
Over the past few decades, new cold plasma sources have been developed that great advantage of operating at atmospheric pressure and temperatures tolerable by biological material. New applications for these emerged, especially in field dermatology. Recently it was demonstrated atmospheric-pressure positively influences healing chronic wounds. The potential lies its capacity to reduce bacterial load wound while same time stimulating skin cells therefore promoting closure. In recent years,...
Cold atmospheric pressure plasma jets (plasma) operated in ambient air provide a rich source of reactive oxygen and nitrogen species (RONS), which are known to influence biological processes important disease. In the treatment diseased tissue such as subcutaneous cancer tumors, RONS need first traverse an interface between plasma-skin surface second be transported millimeter depths order reach deep-seated cells. However, mechanisms generation within soft tissues not understood. this study,...
Modern non-thermal atmospheric pressure plasma sources enable controllable interaction with biological systems. Their future applications - e.g. wound management are based on their unique mixture of reactive components sparking both stimulatory as well inhibitory processes. To gain detailed understanding plasma-cell and respect to risk awareness, key mechanisms need be identified. This study focuses the impact an argon jet (kINPen 09) human HaCaT keratinocytes. With increasing duration, cell...
Non-thermal atmospheric pressure plasma has recently gained attention in the field of biomedical and clinical applications. In area medicine research, one promising approach is to promote wound healing by stimulation cells involved. To understand basic molecular cellular mechanisms triggered treatment, we investigated biological effects an argon jet kinpen on human epithelial skin cells. For assessment transcriptome changes cell culture medium was treated applied HaCaT keratinocyte (indirect...
Abstract Cold plasma has become a promising application in the fields of biology and medicine. Its anti‐microbial effects stimulating properties on eukaryotic cells make an encouraging option treatment chronic wounds. Apart from fibroblasts or keratinocytes, immune have major contribution wound healing. In this study, human peripheral blood mononuclear (PBMC) were tested for viability proliferation after with atmospheric pressure argon jet. time dependent manner compromised PBMC T could be...
The influence of ambient air species especially humidity is an ever-present challenge for atmospheric pressure plasma jet applications. Especially, where the plasma-induced effects are extremely sensitive to humidity, such as in field medicine, understanding diffusion on chemistry and reactive component composition crucial. In this paper, we investigate versus feed gas production components by jets. By use a shielding curtain, control surrounding atmosphere around active effluent region...
In plasma medicine, cold physical delivers a delicate mixture of reactive components to cells and tissues. Recent studies suggested beneficial role in wound healing. Yet, the biological processes related redox modulation via are not fully understood. We here used monocytic cell line THP-1 as model test their response vitro. Intriguingly, short term treatment stimulated growth. Longer exposure only modestly compromised viability but apparently supported growth that were enlarged size showed...
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