A5013017488- Autophagy in Disease and Therapy
- Chemical Synthesis and Analysis
- Multicomponent Synthesis of Heterocycles
- Ubiquitin and proteasome pathways
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Hedgehog Signaling Pathway Studies
- Hippo pathway signaling and YAP/TAZ
- Angiogenesis and VEGF in Cancer
- Signaling Pathways in Disease
- RNA modifications and cancer
- X-ray Diffraction in Crystallography
- Bone and Joint Diseases
- Crystal Structures and Properties
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Chemical and Physical Properties in Aqueous Solutions
- Cancer, Hypoxia, and Metabolism
- Wound Healing and Treatments
- Nutrition and Health in Aging
- CRISPR and Genetic Engineering
- Adipose Tissue and Metabolism
- Cancer-related gene regulation
- T-cell and B-cell Immunology
- Synthesis of Tetrazole Derivatives
The University of Texas Southwestern Medical Center
2010-2025
Shanghai University
2025
Tongji University
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Southwestern Medical Center
2020-2023
Chinese People's Liberation Army
2023
Io Therapeutics (United States)
2023
Second Affiliated Hospital of Zhejiang University
2023
Central South University
2018-2022
Xiangya Hospital Central South University
2018-2022
Intestinal stem cells (ISCs) in the Drosophila adult midgut are essential for maintaining tissue homeostasis and replenishing lost response to damage. Here we demonstrate that Hippo (Hpo) signaling pathway, an evolutionarily conserved pathway implicated organ size control tumorigenesis, plays role regulating ISC proliferation. Loss of Hpo either precursor or epithelial stimulates We provide evidence loss increases production cytokines Upd family multiple EGFR ligands activate JAK-STAT...
L-AgÅPs-gel represents a highly effective and safe antibacterial anti-inflammatory material for promoting wound healing.
Recently, the gut microbiota (GM) has been shown to be a regulator of bone homeostasis and mechanisms by which GM modulates mass are still being investigated. Here, it is found that colonization with from children (CGM) but not elderly (EGM) prevents decreases in strength conventionally raised, ovariectomy (OVX)-induced osteoporotic mice. 16S rRNA gene sequencing reveals CGM reverses OVX-induced reduction Akkermansia muciniphila (Akk). Direct replenishment Akk sufficient correct imbalanced...
Osteonecrosis of the femoral head (ONFH) commonly occurs after glucocorticoid (GC) therapy. The gut microbiota (GM) participates in regulating host health, and its composition can be altered by GC. Here, this study demonstrates that cohousing with healthy mice or colonization GM from normal attenuates GC-induced ONFH. 16S rRNA gene sequencing shows rescues reduction Lactobacillus animalis. Oral supplementation L. animalis mitigates ONFH increasing angiogenesis, augmenting osteogenesis,...
Tissues larger than 400 μm in size lacking microvascular networks cannot survive for long periods of time vitro. The development microfluidic technology provides an efficient research tool constructing models However, traditional single-layer chips faced the limitation spatial layout and could not provide diverse fluidic environments within a single chip. In this paper, we present novel chip design with 3-layer configuration that utilizes polycarbonate (PC) porous membrane to separate...
Myelin-forming oligodendrocytes facilitate saltatory nerve conduction and support neuronal functions in the mammalian CNS. Although processes of oligodendrogliogenesis differentiation from neural progenitor cells have come to light recent years, molecular mechanisms underlying oligodendrocyte myelinogenesis are poorly defined. Herein, we demonstrate pivotal role basic helix-loop-helix transcription factor, Olig1, brain development. Mice lacking a functional Olig1 gene develop severe...
Hedgehog (Hh) signaling regulates embryonic development and adult tissue homeostasis through the GPCR-like protein Smoothened (Smo), but how vertebrate Smo is activated remains poorly understood. In Drosophila, Hh dependent phosphorylation activates Smo. Whether this also case in vertebrates unclear, owing to marked sequence divergence between Drosophila (dSmo) involvement of primary cilia signaling. Here we demonstrate that mammalian (mSmo) multi-site its carboxyl-terminal tail by CK1α...
All three together now: Three-component reactions of aldehydes, anilines, and α-isocyanoacetamides in the presence a catalytic amount chiral phosphoric acid afforded 5-(1-aminoalkyl)-5-aminooxazole 1 excellent yields moderate to good enantiomeric excess (see scheme).
The Cul3-based E3 ubiquitin ligases regulate many cellular processes using a large family of BTB domain–containing proteins as their target recognition components, but how they recognize targets remains unknown. Here we identify and characterize degrons that mediate the degradation Hedgehog pathway transcription factor cubitus interruptus (Ci)/Gli by Cul3-Hedghog–induced MATH protein (HIB)/SPOP. Ci uses multiple Ser/Thr (S/T)-rich motifs bind HIB cooperatively to its degradation. We provide...
Hedgehog transduces signal by promoting cell surface expression of the seven-transmembrane protein Smoothened (Smo) in Drosophila, but underlying mechanism remains unknown. Here we demonstrate that Smo is downregulated ubiquitin-mediated endocytosis and degradation, Hh increases inhibiting its ubiquitination. We find ubiquitinated at multiple Lysine residues including those autoinhibitory domain (SAID), leading to degradation both lysosome- proteasome-dependent mechanisms. inhibits...
Senile osteoporosis (OP) is often concomitant with decreased autophagic activity. OPTN (optineurin), a macroautophagy/autophagy (hereinafter referred to as autophagy) receptor, found play pivotal role in selective autophagy, coupling autophagy bone metabolism. However, its osteogenesis still mysterious. Herein, we identified Optn critical molecule of cell fate decision for marrow mesenchymal stem cells (MSCs), whose expression aged mice. Aged mice revealed osteoporotic loss, elevated...
Alzheimer's disease (AD) is currently ranked as the third leading cause of death for eldly people, just behind heart and cancer. Autophagy declined with aging. Our study determined biphasic changes miR-331-3p miR-9-5p associated AD progression in APPswe/PS1dE9 mouse model demonstrated inhibiting treatment prevented by promoting autophagic clearance amyloid beta (Aβ). Methods: The microRNAs were obtained from RNA-seq data verified qRT-PCR early-stage (6 months) late-stage (12 mice...
Abstract Osteoporosis is a debilitating bone disease affecting millions of people. Here, we used human urine-derived stem cells (USCs), which were noninvasively harvested from unlimited and easily available urine, as “factory” to obtain extracellular vesicles (USC-EVs) demonstrated that the systemic injection USC-EVs effectively alleviates loss maintains strength in osteoporotic mice by enhancing osteoblastic formation suppressing osteoclastic resorption. More importantly, anti-osteoporotic...
Poor wound healing affects millions of people worldwide each year and needs better therapeutic strategies.Synechococcus elongatus PCC 7942 is a naturally occurring photoautotrophic cyanobacterium that can be easily obtained large-scale expanded.Here, we investigated the efficacy this in mouse model acute burn injury whether secretion extracellular vesicles (EVs), important mediators cell paracrine activity, key mechanism cyanobacterium-induced regulation healing.Methods: The effects...
Abstract A differentiation switch of bone marrow mesenchymal stem/stromal cells (BMSCs) from osteoblasts to adipocytes contributes age‐ and menopause‐associated loss adiposity. Here it is found that osteocytes, the most abundant cells, promote adipogenesis inhibit osteogenesis BMSCs by secreting neuropeptide Y (NPY), whose expression increases with aging osteoporosis. Deletion NPY in osteocytes generates a high mass phenotype, attenuates aging‐ ovariectomy (OVX)‐induced bone‐fat imbalance...
Reactive oxygen species (ROS) increase in activated T cells because of metabolic activity induced to support cell proliferation and differentiation. We show that these ROS trigger an oxidative stress response leads translation repression. This is countered by Schlafen 2 (SLFN2), which directly binds transfer RNAs (tRNAs) protect them from cleavage the ribonuclease angiogenin. cell-specific SLFN2 deficiency results accumulation tRNA fragments, inhibit promote stress-granule formation....
Summary VISTA is a key immune checkpoint receptor under investigation for cancer immunotherapy; however, its signaling mechanisms remain unclear. Here we identify conserved four amino acid (NPGF) intracellular motif in that suppresses cell proliferation by constraining cell-intrinsic growth signaling. The NPGF binds to the adapter protein NUMB and recruits Rab11 endosomal recycling machinery. We characterize class of triple-negative breast cancers with high expression low proliferative...
Three-part harmony: Three-component Passerini reactions (P-3CR, see Scheme) of a wide range aldehydes 2 and isocyanides 3, with hydrazoic acid 4 in toluene, the presence [(salen)AlMe]-complex catalyst afford 5-(1-hydroxyalkyl)tetrazoles 1 good-to-excellent yields enantiomeric excess. A tandem Michael addition/enantioselective P-3CR, using an acrolein substrate, affords highly functionalized tetrazoles.
A chiral Lewis acid catalyst was prepared by mixing 2 equiv of binol-derived organophosphoric and 1 Et2AlCl. In the presence a catalytic amount [4j]2Al(III)Cl complex (0.05 equiv), reaction between α-isocyanoacetamides (2) aldehydes (3) afforded corresponding 5-aminooxazoles (1) in good yields enantioselectivities. Complex isolated as white solid displayed similar reactivity that situ.
Casein kinase 2 (CK2) is a typical serine/threonine consisting of α and β subunits has been implicated in many cellular developmental processes. In this study, we demonstrate that CK2 positive regulator the Hedgehog (Hh) signal transduction pathway. We found inactivation by CK2β RNAi enhances loss-of-Hh wing phenotype induced dominant negative form Smoothened (Smo). attenuates Hh-induced Smo accumulation down-regulates Hh target gene expression, whereas increasing activity coexpressing CK2α...
With the wide availability of massively parallel sequencing technologies, genetic mapping has become rate limiting step in mammalian forward genetics. Here we introduce a method for real-time identification N-ethyl-N-nitrosourea-induced mutations that cause phenotypes mice. All are identified by whole exome G1 progenitor and their zygosity is established G2/G3 mice before phenotypic assessment. Quantitative qualitative traits, including lethal effects, single or multiple combined pedigrees...
Designing artificial macromolecules with absolute sequence order is still a long-term challenge in polymer chemistry as opposed to natural biopolymers perfectly defined sequences like proteins and DNA. Herein, we combined amino acid building blocks iterative Ugi reactions for the de novo design synthesis of sequence-defined peptoids. The highly efficient strategy provided excellent yields enables multigram-scale This new furnishes broad structural diversity side chains, well backbones....
Improving revascularization is one of the major measures in fracture treatment. Moderate local inflammation triggers angiogenesis, whereas systemic hampers angiogenesis. Previous studies showed that Akkermansia muciniphila, a gut probiotic, ameliorates by tightening intestinal barrier. In this study, fractured mice intragastrically administrated with A. muciniphila were found to display better healing than treated vehicle. Notably, more preosteclasts positive for platelet-derived growth...