A5070328472- Glycosylation and Glycoproteins Research
- Viral Infections and Immunology Research
- Enzyme Production and Characterization
- Enzyme Structure and Function
- Carbohydrate Chemistry and Synthesis
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Tuberculosis Research and Epidemiology
- Endoplasmic Reticulum Stress and Disease
- Biochemical and Molecular Research
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- Mycobacterium research and diagnosis
- Parvovirus B19 Infection Studies
- RNA and protein synthesis mechanisms
- Microbial Metabolites in Food Biotechnology
- Glycogen Storage Diseases and Myoclonus
- Bacteriophages and microbial interactions
- Metabolism, Diabetes, and Cancer
- Plant-based Medicinal Research
- Galectins and Cancer Biology
- Bacterial Identification and Susceptibility Testing
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
CIC bioGUNE
2016-2024
Hospital de Cruces
2023-2024
University of the Basque Country
2015-2024
BioCruces Health research Institute
2022-2024
Instituto Biofisika
2024
Euskadiko Parke Teknologikoa
2017
Consejo Superior de Investigaciones Científicas
2015-2016
Pennsylvania State University
2010-2014
Instituto de Biotecnología y Biología Molecular
2008-2011
Consejo Nacional de Investigaciones Científicas y Técnicas
2008-2011
The adaptation of viruses to new hosts is a poorly understood process likely involving variety viral structures and functions that allow efficient replication spread. Canine parvovirus (CPV) emerged in the late 1970s as host-range variant virus related feline panleukopenia (FPV). Within few years its emergence dogs, there was worldwide replacement initial strain (CPV type 2) by 2a) characterized four amino acid differences capsid protein. However, evolutionary processes underlie acquisition...
Enterovirus 71 (EV71) is a picornavirus that causes outbreaks of hand, foot, and mouth disease (HFMD), primarily in the Asia-Pacific area. Unlike coxsackievirus A16, which also HFMD, EV71 induces severe neuropathology leading to high fatalities, especially among children under age 6 years. Currently, no established vaccines or treatments are available against infection. The monoclonal antibody MA28-7 neutralizes only specific strains have conserved glycine at amino acid VP1-145,...
Bacterial pathogens have evolved intricate mechanisms to evade the human immune system, including production of immunomodulatory enzymes. Streptococcus pyogenes serotypes secrete two multi-modular endo-β-N-acetylglucosaminidases, EndoS and EndoS2, that specifically deglycosylate conserved N-glycan at Asn297 on IgG Fc, disabling antibody-mediated effector functions. Amongst thousands known carbohydrate-active enzymes, EndoS2 represent just a handful enzymes are specific protein portion...
Bacterial capsules have critical roles in host-pathogen interactions. They provide a protective envelope against host recognition, leading to immune evasion and bacterial survival. Here we define the capsule biosynthesis pathway of Haemophilus influenzae serotype b (Hib), Gram-negative bacterium that causes severe infections infants children. Reconstitution this enabled fermentation-free production Hib vaccine antigens starting from widely available precursors detailed characterization...
Abstract Capsules are long-chain carbohydrate polymers that envelop the surfaces of many bacteria, protecting them from host immune responses. Capsule biosynthesis enzymes potential drug targets and valuable biotechnological tools for generating vaccine antigens. Despite their importance, it remains unknown how structurally variable capsule Gram-negative pathogens linked to conserved glycolipid anchoring these virulence factors bacterial membrane. Using Actinobacillus pleuropneumoniae as an...
ABSTRACT The coxsackievirus-adenovirus receptor (CAR) and decay-accelerating factor (DAF) have been identified as cellular receptors for coxsackievirus B3 (CVB3). first described DAF-binding isolate was obtained during passage of the prototype strain, Nancy, on rhabdomyosarcoma (RD) cells, which express DAF but very little CAR. Here, structure resulting variant, CVB3-RD, has solved by X-ray crystallography to 2.74 Å, a cryo-electron microscopy reconstruction CVB3-RD complexed with refined...
Canine parvovirus (CPV) is an important pathogen causing severe diseases in dogs, including acute hemorrhagic enteritis, myocarditis, and cerebellar disease. Cross-species transmission of CPV occurs as a result mutations on the viral capsid surface that alter species-specific binding to host receptor, transferrin receptor type-1 (TfR). The interaction between TfR has been extensively studied, previous analyses have suggested CPV–TfR complex asymmetric. To enhance understanding underlying...
Abstract The human gut microbiota plays a central role not only in regulating the metabolism of nutrients but also promoting immune homeostasis, responses and protection against pathogen colonization. genome Gram-negative symbiont Bacteroides thetaiotaomicron , dominant member intestinal microbiota, encodes polysaccharide utilization loci PULs, apparatus required to orchestrate degradation specific glycan. EndoBT-3987 is key endo-β- N -acetylglucosaminidase (ENGase) that initiates...
Abstract Red blood cell antigens play critical roles in transfusion since donor incompatibilities can be lethal. Recipients with the rare total deficiency H antigen, O h Bombay phenotype, only transfused group to avoid serious reactions. We discover FucOB from mucin-degrading bacteria Akkermansia muciniphila as an α-1,2-fucosidase able hydrolyze Type I, II, III and V obtain afucosylated phenotype vitro. X-ray crystal structures of show a three-domain architecture, including GH95 glycoside...
ABSTRACT Enterovirus 71 (EV71) is an important emerging human pathogen with a global distribution and presents disease pattern resembling poliomyelitis seasonal epidemics that include cases of severe neurological complications, such as acute flaccid paralysis. EV71 member the Picornaviridae family, which consists icosahedral, nonenveloped, single-stranded RNA viruses. Here we report structures derived from X-ray crystallography cryoelectron microscopy (cryo-EM) for 1095 strain EV71,...
Abstract Glycosyltransferases (GTs) comprise a prominent family of enzymes that play critical roles in variety cellular processes, including cell signaling, development, and host–pathogen interactions. Glycosyl transfer can proceed with either inversion or retention the anomeric configuration respect to reaction substrates products. The elucidation catalytic mechanism retaining GTs remains major challenge. A native ternary complex GT productive mode for catalysis is reported,...
ABSTRACT Canine parvovirus (CPV) and its relative feline panleukopenia virus (FPV) bind the transferrin receptor type 1 (TfR) to infect their host cells but show differences in interactions with canine TfRs that determine viral range tissue tropism. We changed apical protease-like domain residues by introducing point mutations adding or removing glycosylation signals, we then examined of those mutant capsids. Most substitutions had little effect on binding uptake. However, several sites...
ABSTRACT Echovirus 7 (EV7) belongs to the Enterovirus genus within family Picornaviridae . Many picornaviruses use IgG-like receptors that bind in viral canyon and are required initiate uncoating during infection. However, addition, some of enteroviruses an alternative or additional receptor binds outside canyon. Decay-accelerating factor (DAF) has been identified as a cellular for EV7. The crystal structure EV7 determined 3.1-Å resolution used interpret 7.2-Å-resolution cryo-electron...
Many coxsackievirus B isolates bind to human decay-accelerating factor (DAF) as well the and adenovirus receptor (CAR). The first-described DAF-binding isolate, B3 (CB3)-RD, was obtained during passage of prototype strain CB3-Nancy on RD cells, which express DAF but very little CAR. CB3-RD binds DAF, whereas does not. To determine molecular basis for specific interaction with we produced cDNA clones encoding both mutated each sites at Nancy sequences diverged. We found that a single amino...
Abstract The biosynthesis of phospholipids and glycolipids are critical pathways for virtually all cell membranes. PatA is an essential membrane associated acyltransferase involved in the mycobacterial phosphatidyl- myo -inositol mannosides (PIMs). enzyme transfers a palmitoyl moiety from palmitoyl–CoA to 6-position mannose ring linked 2-position inositol PIM 1 /PIM 2 . We report here crystal structures Mycobacterium smegmatis presence its naturally occurring acyl donor palmitate...
Bacteroidales (syn. Bacteroidetes) are prominent members of the human gastrointestinal ecosystem mainly due to their efficient glycan-degrading machinery, organized into gene clusters known as polysaccharide utilization loci (PULs). A single PUL was reported for catabolism high-mannose (HM) N-glycan glyco-polypeptides in gut symbiont Bacteroides thetaiotaomicron, encoding a surface endo-β-N-acetylglucosaminidase (ENGase), BT3987. Here, we discover an ENGase from GH18 family B. BT1285,...
Enterovirus 71 (EV71) is responsible for seasonal outbreaks of hand, foot, and mouth disease in the Asia-Pacific region. The virus has capability to cause severe death, especially young children. Although several vaccines are currently clinical trials, no or therapeutics have been approved use. Previous structural studies revealed that two antigenically distinct capsid forms produced EV71-infected cells: an expanded empty capsid, sometimes called a procapsid, infectious virus. Specifically,...
PlsX plays a central role in the coordination of fatty acid and phospholipid biosynthesis Gram-positive bacteria. is peripheral membrane acyltransferase that catalyzes conversion acyl-ACP to acyl-phosphate, which turn utilized by polytopic PlsY on pathway bacterial biosynthesis. We have recently studied interaction between phospholipids vivo vitro, observed association necessary for efficient transfer acyl-phosphate PlsY. However, understanding molecular basis such channeling mechanism...
Abstract To understand better how different genomic regions may confer pathogenicity for the coxsackievirus B (CVB), two intratypic CVB1 variants, and a number of recombinant viruses were studied. Sequencing analysis showed 23 nucleotide changes between parental non‐pathogenic CVB1N pathogenic CVB1Nm. Mutations present in CVB1Nm more conserved than those when compared to other CVB sequences. Inoculation C3H/HeJ mice that P1 region is critical murine pancreas heart. The molecular determinants...