A5071623301- Marine Toxins and Detection Methods
- Nicotinic Acetylcholine Receptors Study
- Marine and coastal ecosystems
- Environmental Toxicology and Ecotoxicology
- Animal Ecology and Behavior Studies
- Analytical chemistry methods development
- Forensic Toxicology and Drug Analysis
- Marine Sponges and Natural Products
- Analytical Chemistry and Chromatography
- Aquatic Ecosystems and Phytoplankton Dynamics
- Environmental Chemistry and Analysis
- Hemoglobin structure and function
- Advanced Chemical Sensor Technologies
- Water Quality Monitoring and Analysis
- Mass Spectrometry Techniques and Applications
- Analytical Methods in Pharmaceuticals
- Marine Biology and Environmental Chemistry
- Isotope Analysis in Ecology
- Nitric Oxide and Endothelin Effects
- Marine Invertebrate Physiology and Ecology
- Pesticide Residue Analysis and Safety
- Chemical synthesis and alkaloids
- Molecular Sensors and Ion Detection
- Aquaculture disease management and microbiota
- Metabolomics and Mass Spectrometry Studies
National Research Council Canada
2014-2024
Dalhousie University
2014
Institute for Marine Biosciences
2011
LC/MS methodology for the analysis of domoic acid and lipophilic toxins in shellfish was developed using a hybrid triple quadrupole linear ion trap mass spectrometer. For routine quantitation scheduled selected reaction monitoring (SRM) method acid, okadaic dinophysistoxins, azaspiracids, pectenotoxins, yessotoxins, gymnodimines, spirolides, pinnatoxins validated. The performed well terms LOD, linearity, precision, trueness. Taking advantage high instrument sensitivity, matrix effects were...
Main text The CCQM-K148.b comparison, undertaken with a parallel pilot study CCQM-P187.b, was coordinated by the BIPM and UME on behalf of Consultative Committee for Amount Substance: Metrology in Chemistry Biology (CCQM) Working Group Organic Analysis (OAWG). It National Measurement Institutes (NMIs) Designated (DIs) which provide measurement services organic analysis under CIPM Mutual Recognition Arrangement (MRA) designated Track A comparison within OAWG implementation CCQM Strategy...
The goal of this research was to develop a robust method for taste and odour compounds that can be implemented by laboratories with mass spectrometers lacking chemical ionisation capabilities or specialised sample introduction hardware are commonly used methods. Development, optimisation, validation solid-phase extraction using liquid injection gas chromatography – tandem spectrometry detection electron impact described. Camphor as an internal standard, through development robustness testing...
Paralytic shellfish toxins (PSTs) are a complex class of analogs the potent neurotoxin saxitoxin (STX). Since calibration standards not available for many PSTs, including C-11 hydroxyl called M-toxins, accurate quantitation by liquid chromatography–mass spectrometry (LC-MS) can be challenging. In absence standards, PSTs often semiquantitated using different analog (e.g., STX), an approach with high degree uncertainty due to highly variable sensitivity between analytes in electrospray...
Okadaic acid (OA) group toxins may accumulate in shellfish and can result diarrhetic poisoning when consumed by humans, are therefore regulated. Purified required for the production of certified reference materials used to accurately quantitate toxin levels water samples, other research purposes. An improved procedure was developed isolation dinophysistoxin 2 (DTX2) from (M. edulis), reducing number purification steps eight five, thereby increasing recoveries ~68%, compared ~40% a previously...
The presence of azaspiracids (AZAs) in shellfish may cause food poisoning humans. AZAs can accumulate filtering seawater that contains marine dinoflagellates such as Azadinium and Amphidoma spp. More than 60 AZA analogues have been identified, which AZA1, AZA2 AZA3 are regulated Europe. Shellfish matrices complicate quantitation by ELISA LC–MS methods. Polyclonal antibodies developed bind specifically to the C-26–C-40 domain structure could potentially be used for selectively extracting...
Azaspiracids (AZAs) are a group of biotoxins produced by the marine dinoflagellates Azadinium and Amphidoma spp. that can accumulate in shellfish cause food poisoning humans. Of 60 AZAs identified, levels AZA1, AZA2, AZA3 regulated as safety measure based on occurrence toxicity. Information about metabolism is limited. Therefore, fraction blue mussel hepatopancreas was made to study AZA1–3 vitro. A range AZA metabolites were detected liquid chromatography–high-resolution tandem mass...
Microcystins (MCs) are a potent class of hepatotoxins produced by several species cyanobacteria, which have been responsible for illness and death in livestock humans.
Liquid chromatography–high-resolution mass spectrometry (LC-HRMS) analysis of a Namibian strain Gonyaulax spinifera showed the presence number yessotoxins (YTXs). Principal among these were YTX (1), homoYTX (2), and tentative hydroxylated analogue that did not correspond to any previously confirmed structures. Culturing G. afforded sufficient biomass for purification new through series solvent partitioning chromatographic steps, yielding ∼0.9 mg as solid. NMR spectroscopy, ion-trap...
Two high-mass polar compounds were observed in aqueous side-fractions from the purification of okadaic acid (1) and dinophysistoxin-2 (2) Dinophysis blooms Spain Norway. These isolated shown to be 24-O-β-d-glucosides 1 2 (4 5, respectively) by nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry, enzymatic hydrolysis. These, together with standards 1, 2, dinophysistoxin-1 (3), a synthetic specimen 7-deoxy-1 (7), combined an understanding their spectrometric fragmentation...
Azaspiracids (AZAs) are a group of polyether marine algal toxins known to accumulate in shellfish, posing risk human health and the seafood industry. Analysis AZAs is typically performed using LC-MS, which can suffer from matrix effects that significantly impact accuracy measurement results. While use isotopic internal standards an effective approach correct for these effects, isotopically labelled not currently available. In this study, 18O-labelled AZA1, AZA2, AZA3 were prepared by...
The presence of azaspiracids (AZAs) in shellfish may cause food poisoning humans. AZAs can accumulate filtering seawater that contains marine dinoflagellates such as Azadinium and Amphidoma spp. More than 60 AZA analogues have been identified, which AZA1, AZA2 AZA3 are regulated Europe. Shellfish matrices complicate quantitation by ELISA LC–MS methods. Polyclonal antibodies developed bind specifically to the C-26–C-40 domain structure could potentially be used for selectively extracting...