A5067104221- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
- Metabolomics and Mass Spectrometry Studies
- NMR spectroscopy and applications
- Cancer, Lipids, and Metabolism
- Food Chemistry and Fat Analysis
- Antimicrobial Peptides and Activities
- Chromatography in Natural Products
- Supramolecular Self-Assembly in Materials
- Natural product bioactivities and synthesis
- Biochemical and Structural Characterization
- Plant-based Medicinal Research
- RNA Interference and Gene Delivery
- Advanced NMR Techniques and Applications
- Sphingolipid Metabolism and Signaling
- Cancer, Hypoxia, and Metabolism
- Carbohydrate Chemistry and Synthesis
- Biological Activity of Diterpenoids and Biflavonoids
- Prostate Cancer Treatment and Research
- Curcumin's Biomedical Applications
- Diet and metabolism studies
- Microbial Natural Products and Biosynthesis
- Diet, Metabolism, and Disease
- Proteins in Food Systems
- Spectroscopy and Quantum Chemical Studies
Baker Heart and Diabetes Institute
2021-2024
The University of Melbourne
2016-2024
La Trobe University
2023
RMIT University
2018-2021
MIT University
2021
CSIRO Manufacturing
2016-2017
Herein, we demonstrate a method for the functionalization of cubic phase lipid nanoparticles (cubosomes) with series magnetite (Fe3O4), copper oxide (Cu2O), and silver (Ag) nanocrystals, prospective applications across wide range fields, including antimicrobial treatments. The resulting cubosomes are characterized using small-angle X-ray scattering dynamic light scattering, demonstrating retention typical structure particle size following nanocrystal encapsulation at concentrations up to 20%...
This study investigates the use of lipid nanoparticles as a delivery vehicle for curcumin. We explore localization curcumin within bilayer, entrapment efficiency, resulting nanoparticle morphology and cytotoxicity.
Obesity is a risk factor for type 2 diabetes and cardiovascular disease. However, substantial proportion of patients with these conditions have seemingly normal body mass index (BMI). Conversely, not all obese individuals present metabolic disorders giving rise to the concept "metabolically healthy obese". We use lipidomic-based models BMI calculate score (mBMI) as measure dysregulation associated obesity. Using difference between mBMI (mBMIΔ), we identify similar but differing in their...
Accurate risk stratification is vital for primary prevention of cardiovascular disease (CVD). However, traditional tools such as the Framingham Risk Score (FRS) may underperform within diverse intermediate-risk group, which includes individuals requiring distinct management strategies. This study aimed to develop a lipidomic-enhanced score (LRS), specifically targeting prediction and reclassification intermediate benchmarked against FRS. The LRS was developed via machine learning workflow...
Intrinsic resistance to androgen receptor signalling inhibitors (ARSI) occurs in 20-30% of men with metastatic castration-resistant prostate cancer (mCRPC). Ceramide metabolism may have a role ARSI resistance. Our study's aim is investigate the association ceramide-sphingosine-1-phosphate (ceramide-S1P) axis mCRPC.Lipidomic analysis (∼700 lipids) was performed on plasma collected from 132 mCRPC, before commencing enzalutamide or abiraterone. AR gene aberrations 77 these were identified by...
The ordered nanostructured lipidic bicontinuous cubic phase has demonstrated potential as a drug release material, due to its ability encapsulate wide variety of compounds, which may undergo sustained, diffusion controlled over time. Control been shown depend on the nanostructural parameters lipid mesophase. Herein, and two amino acids, encapsulated within range different mesophases are investigated. Pulsed-field gradient NMR was used determine coefficient acid, found be correlated with...
Abstract Background Both changes in circulating lipids represented by a validated poor prognostic 3-lipid signature (3LS) and somatic tumour genetic aberrations are individually associated with worse clinical outcomes men metastatic castration-resistant prostate cancer (mCRPC). A key question is how the lipid environment genome interrelated order to exploit this therapeutically. We assessed association between (3LS), mCRPC. Methods performed plasma lipidomic analysis cell-free DNA (cfDNA)...
The structural changes occurring at the nanoscale level within lipid bilayer and driving in-meso formation of large well-diffracting membrane protein crystals have been uniquely characterized for a model protein, intimin. Importantly, order to transitions taking place lipidic nanostructures required crystal growth shown be general, both cubic sponge mesophase crystallization pathways. For first time, transient fluid lamellar phase has observed unambiguously assigned pathways, present...
Lipid based bicontinuous cubic mesophases provide a low-cost, robust membrane mimetic nanomaterial which allows for the incorporation of peptides and proteins.
Nanostructured bicontinuous lipidic cubic phases are used for the encapsulation of proteins in a range applications such as meso crystallization transmembrane and drug delivery vehicles. The retention nanoscale order subsequent to protein incorporation, well structure function, is essential all these applications. Herein synthetic peptides (WALP21, WALPS53, WALPS73) with common α-helical hydrophobic domain, but varying hydrophilic loop size, were designed systematically examine effect...
Circulating lipids or cytokines are associated with prognosis in metastatic castration-resistant prostate cancer (mCRPC). This study aimed to understand the interactions between lipid metabolism and immune response mCRPC by investigating relationship plasma lipidome cytokines. Plasma samples from two independent cohorts of men (n = 146, 139) having life-prolonging treatments were subjected lipidomic cytokine profiling (290, 763 lipids; 40 cytokines). Higher baseline levels sphingolipids,...
Obtaining well-diffracting crystals of membrane proteins, which is crucial to the molecular-level understanding their intrinsic three-dimensional structure, dynamics, and function, represents a fundamental bottleneck in field structural biology. One major advances protein determination was realization that nanostructured lipidic cubic phase (LCP) environment constitutes mimetic matrix promotes solubilization, stabilization, crystallization specific proteins. Despite two decades passing since...
For evolving biological and biomedical applications of hybrid protein?lipid materials, understanding the behavior protein within lipid mesophase is crucial. After more than two decades since invention in meso crystallization method, a protein-eye view its mechanism still lacking. Numerous structural studies have suggested that integral membrane proteins preferentially partition at localized flat points on bilayer surface cubic phase with crystal growth occurring from local fluid lamellar L?...
The antibiotic uliginosin A-iBiB has been synthesised by a rottlerone exchange between albaspidin-iBiB and 2′,4′,6′-trihydroxy-3′-(3-methylbut-2-enyl)isobutyrophenone. Natural A contains homologue (M+ 14) so analogues, in which each of the isobutyryl groups is replaced isovaleryl, have prepared; A-iViB probably minor component natural A. Dihydrouliginosin B-iBiB isodihydrouliginosin also prepared.
Lipidic inverse bicontinuous cubic phases (LCPs), formed via the spontaneous self-assembly of lipids such as monoolein, have found increasing applications in stabilization and crystallization integral membrane proteins for structural characterization using X-ray crystallography. Their use effective drug release matrices has also been demonstrated. Nuclear magnetic resonance (NMR) spectroscopy, both solution solid state, previously employed LCPs related systems. Herein, we report a number...
The crystallization of oil droplets is critical in the processing and storage lipid-based food pharmaceutical products. Arrays femtoliter on a surface offer unique opportunity to study surfactant-free colloidlike systems. In this work, crystal growth process these confined was followed by cooling model lipid (trimyristin) from liquid state utilizing synchrotron small-angle X-ray scattering (SAXS). measurements SAXS demonstrated reduced rate greater degree supercooling required trigger...
Several routes to 5,7-dihydroxy-8-isobutyryl-2,2-dimethyl-2H-chromen were investigated and it was eventually prepared, together with the 6-isobutyryl isomer, by cyclodehydrogenation of 2′,4′,6′-trihydroxy-3′-(3-methylbut-2-enyl)isobutyrophenone using DDQ. A rottlerone exchange between 8-isobutyrylchromen albaspidin-iBiB then afforded uliginosin B-iBiB. Uliginosin B-iBiB B-iBiV have also been prepared corresponding derivatives A. Natural B contains a homologue (M+ 14) which is probably...
The encapsulation of drugs in nanoparticles serves as an effective way to modify pharmacokinetics and therapeutic efficacy. Nanoparticles comprised poly(d,l-lactide-co-glycolide) (PLGA) are well suited for this purpose; they accessible using multiple synthesis methods, highly biocompatible biodegradable, possess desirable drug release properties. In the present study, we have explored effects various formulation parameters on physical properties PLGA synthesised a microfluidic assisted...