A5067713493- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
- Surfactants and Colloidal Systems
- Supramolecular Self-Assembly in Materials
- Proteins in Food Systems
- Food Chemistry and Fat Analysis
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Glycosylation and Glycoproteins Research
- Receptor Mechanisms and Signaling
- Enzyme Structure and Function
- Spectroscopy and Quantum Chemical Studies
- Advanced biosensing and bioanalysis techniques
- Metabolomics and Mass Spectrometry Studies
- Chemical Synthesis and Analysis
- Nanopore and Nanochannel Transport Studies
- Carbohydrate Chemistry and Synthesis
- Cholesterol and Lipid Metabolism
- Click Chemistry and Applications
- Lanthanide and Transition Metal Complexes
- Force Microscopy Techniques and Applications
- Bacteriophages and microbial interactions
- Child Therapy and Development
RMIT University
2016-2025
University of Bolton
2022-2023
MIT University
2016-2022
CSIRO Manufacturing
2009-2016
Materials Science & Engineering
2010-2014
Commonwealth Scientific and Industrial Research Organisation
2008-2013
RMIT Europe
2009
European Synchrotron Radiation Facility
2008
University of Reading
2008
Imperial College London
2006-2008
In this paper, we give an overview of our studies by static and time-resolved X-ray diffraction inverse cubic phases phase transitions in lipids. §1 , briefly discuss the lyotropic behaviour lipids, focusing attention on non-lamellar structures, their geometric/topological relationship to fusion processes lipid membranes. Possible pathways for between different are also outlined. §2 effects hydrostatic pressure membranes transitions, describe how parameters required predict dependence...
Herein, we demonstrate a method for the functionalization of cubic phase lipid nanoparticles (cubosomes) with series magnetite (Fe3O4), copper oxide (Cu2O), and silver (Ag) nanocrystals, prospective applications across wide range fields, including antimicrobial treatments. The resulting cubosomes are characterized using small-angle X-ray scattering dynamic light scattering, demonstrating retention typical structure particle size following nanocrystal encapsulation at concentrations up to 20%...
We review the use of bicontinuous cubic amphiphile self-assembly materials for protein encapsulation. The unique amphiphilic nature lipidic phases make them an ideal medium encapsulation soluble, peripheral, and integral membrane proteins. Current prospective applications such systems include drug delivery, crystallisation, biosensors biofuel cells. focus on impact incorporated structure phase, which has a knock-on effect performance many end-applications.
Abstract Drug delivery with nanocarriers relies on the interaction of individual cell surface. For lipid-based NCs, this uniquely involves a process membrane fusion between lipid bilayer that makes up NC and membrane. Cubosomes have emerged as promising fusogenic however their interactions had not yet been directly observed due to difficulties in achieving adequate resolution or disentangling multiple common characterization techniques. Moreover, many studies these performed under static...
This study investigates the use of lipid nanoparticles as a delivery vehicle for curcumin. We explore localization curcumin within bilayer, entrapment efficiency, resulting nanoparticle morphology and cytotoxicity.
The liquid crystalline lamellar (${L}_{\ensuremath{\alpha}}$) to double-diamond inverse bicontinuous cubic (${Q}_{\mathrm{II}}^{D}$) phase transition for the amphiphile monoelaidin in excess water exhibits a remarkable sequence of structural transformations pressure or temperature jumps. Our data imply that dynamics depends on coupling between changes molecular shape and geometrical topological constraints domain size. We propose qualitative model this based theories membrane fusion via...
We demonstrate that oral delivery of self-assembled nanostructured nanoparticles consisting 5-fluorouracil (5-FU) lipid prodrugs results in a highly effective, target-activated, chemotherapeutic agent, and offers significantly enhanced efficacy over commercially available alternative does not self-assemble. The prodrug have been found to slow the growth aggressive mouse 4T1 breast tumour, essentially halt human MDA-MB-231 tumour xenografts. Systemic toxicity is avoided as activation requires...
Lipid nanoparticles of internal cubic symmetry, termed cuboplexes, are potential nonviral delivery vehicles for gene therapy due to their "topologically active" nature, which may enhance endosomal escape and improve outcomes. In this study, we have used cationic based on monoolein (MO) doped with a lipid, the encapsulation antisense green fluorescent protein (GFP)—small interfering RNA (siRNA) into Chinese Hamster Ovary (CHO)—GFP cells. Agarose gel electrophoresis has confirmed successful...
This study aimed to understand the structural devolution of 10% w/w rennet-induced (RG) and transglutaminase-induced acid (TG) gels in H2O D2O under vitro gastric conditions with without pepsin. The real-time structure at a nano- (e.g. colloidal calcium phosphate (CCP) micelle) micro- (gel network) level was determined using ultra-small (USANS) small-angle neutron scattering (SANS) electron microscopy. Results demonstrate that gel firmness or elasticity determines disintegration behaviour...
Antibiotic-resistant bacteria pose a significant threat to humanity. Gram-negative strains have demonstrated resistance last resort antibiotics, partially due their outer membrane, which hinders transport of antimicrobials into the bacterium. Nanocarrier (NC)-mediated drug delivery is one proposed strategy for combating this emerging issue. Here, uptake self-assembled lipid nanocarriers cubic symmetry (cubosomes) revealed fundamental differences in mechanism between Gram-positive and...
Nanomaterials have the potential to transform biological and biomedical research, with applications ranging from drug delivery diagnostics targeted interference of specific processes. Most existing research is aimed at developing nanomaterials for tasks such as enhanced biocellular internalization. However, fundamental aspects interactions between systems, in particular, membranes, remain poorly understood. In this study, we provide detailed insights into molecular mechanisms governing...
Lyotropic liquid crystalline nanoparticles (LLCNPs) with complex internal nanostructures hold promise for drug delivery. Cubosomes, in particular, have garnered interest their ability to fuse cell membranes, potentially bypassing endosomal escape challenges and improving cellular uptake. The mesostructure of plays a crucial role interactions Therefore, we hypothesise that the specific mesophase LLCNPs will affect uptake efficiencies, cubosomes exhibiting superior compared other LLCNPs.LLCNPs...
An improved vaccine is urgently needed to replace the now more than 100-year-old Bacillus Calmette-Guérin (BCG) against tuberculosis (TB) disease, which represents a significant burden on global public health. Mycolic acid, or cord factor trehalose 6,6' dimycolate (TDM), lipid component abundant in cell wall of pathogen Mycobacterium (MTB), has been shown have strong immunostimulatory activity but remains underexplored due its high toxicity and poor solubility. Herein, we employed novel...
Inverse bicontinuous cubic lyotropic phases are a complex solution to the dilemma faced by all self-assembled water-amphiphile systems: how satisfy incompatible requirements for uniform interfacial curvature and molecular packing. The reached in this case is interfaces deform hyperbolically onto triply periodic minimal surfaces. We have previously suggested that although packing these structures rather relative phase behavior of gyroid, double diamond, primitive inverse can be understood...
The ordered nanostructured lipidic bicontinuous cubic phase has demonstrated potential as a drug release material, due to its ability encapsulate wide variety of compounds, which may undergo sustained, diffusion controlled over time. Control been shown depend on the nanostructural parameters lipid mesophase. Herein, and two amino acids, encapsulated within range different mesophases are investigated. Pulsed-field gradient NMR was used determine coefficient acid, found be correlated with...
We present the first observation of Janus nanoparticles consisting stable, coexisting ordered mesophases in discrete particles created by lipid self-assembly. Cryo-TEM images provided visual identification multicompartment and, combined with SAXS data, confirmed presence mixed cubic phases and cubic/hexagonal within individual nanoparticles. further investigated computer visualization models to interpret potential interface between interconnected nanostructured domains a single nanoparticle.
Milk has been used commercially as a carrier for phytosterols, but there is limited knowledge on the effect of added plant sterols properties system. In this study, phytosterols dispersed in milk fat at level 0.3 or 0.6% were homogenized with an aqueous dispersion whey protein isolate (WPI). The particle size, morphology, ζ-potential, and stability emulsions investigated. Emulsion crystallization examined through use differential scanning calorimetry (DSC) Synchrotron X-ray scattering both...