A5060559462- Supramolecular Self-Assembly in Materials
- Lipid Membrane Structure and Behavior
- Chemical Synthesis and Analysis
- Polydiacetylene-based materials and applications
- Antimicrobial Peptides and Activities
- Glycosylation and Glycoproteins Research
- Alzheimer's disease research and treatments
- Protein Structure and Dynamics
- Pituitary Gland Disorders and Treatments
- Diatoms and Algae Research
- Advanced Drug Delivery Systems
- Advanced biosensing and bioanalysis techniques
- Force Microscopy Techniques and Applications
- Biochemical and Structural Characterization
- RNA Interference and Gene Delivery
- Genetic Neurodegenerative Diseases
- Porphyrin and Phthalocyanine Chemistry
- Parkinson's Disease Mechanisms and Treatments
- Ovarian function and disorders
- Luminescence and Fluorescent Materials
- Proteins in Food Systems
- Bacteriophages and microbial interactions
- Glycogen Storage Diseases and Myoclonus
- Advanced Electron Microscopy Techniques and Applications
- Nanoparticle-Based Drug Delivery
RMIT University
2014-2024
University of Canterbury
2013-2020
MIT University
2014-2020
Ipsen (Spain)
2004-2013
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2003-2010
CEA Paris-Saclay
2010
Centre National de la Recherche Scientifique
2003-2010
Institut de Physique de Rennes
2010
Ipsen (France)
2007
Laboratoire d'Énergétique Moléculaire et Macroscopique, Combustion
2004
The controlled self-assembly of complex molecules into well defined hierarchical structures is a promising route for fabricating nanostructures. These nanoscale can be realized by naturally occurring proteins such as tobacco mosaic virus, capsid proteins, tubulin, actin, etc. Here, we report simple alternative method based on self-assembling nanotubes formed synthetic therapeutic octapeptide, Lanreotide in water. We used multidisciplinary approach involving optical and electron microscopies,...
Supramolecular self-assembly is an attractive pathway for bottom-up synthesis of novel nanomaterials. In particular, this approach allows the spontaneous formation structures well-defined shapes and monodisperse characteristic sizes. Because nanotechnology mainly relies on size-dependent physical phenomena, control monodispersity required, but possibility tuning size also essential. For self-assembling systems, shape, size, are settled by chemical structure building block. Attempts to change...
Nanofabrication by molecular self-assembly involves the design of molecules and strategies so that shape chemical complementarities drive units to organize spontaneously into desired structures. The power makes it ubiquitous strategy living organized matter provides a powerful tool chemists. However, challenging issue in complex supramolecular structures is understand how kinetically efficient pathways emerge from multitude possible transition states routes. Unfortunately, very few systems...
Specific peptide sequences designed by inspection of protein–protein interfaces have been identified and used as tectons in hybrid functional materials. Here, an 8‐mer derived from interface the peroxiredoxin family self‐assembling proteins is exploited to encode assembly perylene imide‐based organic semiconductor building blocks. By augmenting with additional functionality trigger aggregation manipulate directionality peptide‐semiconductor coupling, a series materials based on natural...
The potential for protein tectons to be used in nanotechnology is increasingly recognized, but the repertoire of stable proteins that assemble into defined shapes response an environmental trigger limited. Peroxiredoxins (Prxs) are a family shows amazing array supramolecular assemblies, making them attractive tectons. Human Prx3 (hPrx3) forms toroidal oligomers characteristic Prx family, no structure has been solved date. Here we report first 3-D this protein, derived from single-particle...
External stimuli are powerful tools that naturally control protein assemblies and functions. For example, during viral entry exit changes in pH known to trigger large conformational changes. However, the molecular features stabilizing higher structures remain unclear. Here we elucidate change of a self-assembling peptide forms either small or nanotubes dependent on pH. The sub-angstrom high-pH structure reveals globular conformation stabilized through strong histidine-serine H-bond tight...
Among noncovalent forces, electrostatic ones are the strongest and possess a rather long-range action. For these reasons, charges counterions play prominent role in self-assembly processes water therefore many biological systems. However, complexity of media often hinders detailed understanding all electrostatic-related events. In this context, we have studied lanreotide, cationic octapeptide. This peptide spontaneously forms monodisperse nanotubes (NTs) above critical concentration when...
Microbial resistance to common antibiotics is threatening cause the next pandemic crisis. In this context, antimicrobial peptides (AMPs) are receiving increased attention as an alternative approach traditional small molecule antibiotics. Here, we report bi-functional rational design of Fmoc-peptides both and hydrogelator substances. The tetrapeptide Fmoc-WWRR-NH2-termed Priscilicidin-was rationally designed for activity molecular self-assembly into nanostructured hydrogels. Molecular...
Peptides can serve as versatile therapeutics with a highly modular structure and tunable biophysical properties. In particular, the efficacy of peptide antibiotics against drug-resistant pathogens is great promise, few new classes are being developed to overcome ever-increasing bacterial resistance contemporary drugs. This work reports antimicrobial studies designed library ultrashort peptides that self-assemble into hydrogels at concentrations low 0.5% w/v in buffered saline, confirmed by...
An investigation of the biophysical stability and safety properties crystallin amyloid nanofibrils – a potential novel bionanomaterial.
Motifs of 7–8 amino acids were designed from the β-continuous interfaces non-related homo-oligomeric proteins. These peptides intrinsically self-assembled into nanoarchitectures in water, while retaining some properties their parent interfaces, especially reversibility assembly. results reveal a novel source native peptide tectons.
Amyloid nanofibrils are β-sheet rich protein or peptide assemblies that have pathological roles in over 20 neurodegenerative diseases, but also can essential physiological roles. This wide variety of functions is likely to be due subtle differences amyloid structure and assembly mechanisms. Glycosaminoglycans (GAGs), like heparin, frequently used vitro increase the kinetics fibrils. However, little known about effects adding large polymeric sugars on mechanisms nanostructures. Here, we...
Gonadotropin-releasing hormone (GnRH) is a short human neuropeptide involved in the regulation of sex hormones. We report that GnRH self-assembles into reversible β-sheet-based nanofibrils, with pH-dependent kinetics. At high concentrations, these nanostructures form arrays arranged liquid crystalline hexagonal phases. Histidine deprotonation increasing pH can mediate formation precipitates. Our results are relevant to functional amyloids general, intracellular storage process within...
Although antimicrobial peptides (AMPs) are a promising class of new antibiotics, their inherent susceptibility to degradation requires nanocarrier-mediated delivery. While cubosome nanocarriers have been extensively studied for delivery AMPs, we do not currently understand why encapsulation improves efficacy some compounds but others. This study therefore aims investigate the link between mechanism action and permeation efficiency peptides, efficacy, activity these systems. Encapsulation...
Self-assembled nanoarchitectures based on biological molecules are attractive because of the simplicity and versatility building blocks. However, size control is still a challenge. This only possible when given system deeply understood. Such case with lanreotide acetate, an octapeptide salt that spontaneously forms monodisperse nanotubes dissolved into pure water. Following structural approach, we have in past demonstrated possibility to tune diameter these while keeping strict...
Abstract We investigated the spectroscopic properties of aromatic residues in a set octapeptides with various self‐assembly properties. These are based on lanreotide, cyclic peptide analogue somatostatin‐14 that spontaneously self‐assembles into very long and monodisperse hollow nanotubes. A previous study these lanreotide‐based derivatives has shown disulfide bridge, hairpin conformation involved process modification either decreases propensity or modifies molecular packing resulting...