A5072205563- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Supramolecular Chemistry and Complexes
- Crystallography and molecular interactions
- Connective tissue disorders research
- Aortic Disease and Treatment Approaches
- Metal-Organic Frameworks: Synthesis and Applications
- Supramolecular Self-Assembly in Materials
- Cardiac Valve Diseases and Treatments
- Chemical Synthesis and Analysis
- RNA Interference and Gene Delivery
- Protein Structure and Dynamics
- Cardiovascular Issues in Pregnancy
- Marine Sponges and Natural Products
- Microbial Natural Products and Biosynthesis
- Protein Kinase Regulation and GTPase Signaling
- Enzyme Structure and Function
- Ubiquitin and proteasome pathways
- Nanomaterials for catalytic reactions
- Peptidase Inhibition and Analysis
- Nanocluster Synthesis and Applications
- Magnetism in coordination complexes
- Fluorine in Organic Chemistry
- Bone health and treatments
- Acute Kidney Injury Research
Kyoto University
2019-2024
The University of Tokyo
2011-2021
Whitehead Institute for Biomedical Research
2017-2018
University of Tokyo Hospital
2018
Japan Science and Technology Agency
2011-2016
St. Marianna University School of Medicine
2016
Pohang University of Science and Technology
2014
RIKEN Advanced Science Institute
2010
Okazaki Institute for Integrative Bioscience
2010
Nagoya City University
2010
Drawing inspiration from the self-assembly of hollow spherical virus capsids and protein cages found in nature, a family roughly coordination polyhedra with general formula MnL2n was designed several members series have been synthesised. These complexes are self-assembled upon reaction bent bis(pyridine) ligands Pd(2+) ions. The introduction functional side chains into is straightforward, making synthesis both exo- endohedrally functionalised possible. Accumulation high density groups at...
Establishment of a general one-pot cascade reaction protocol would dramatically reduce the effort multistep organic synthesis. We demonstrate that unique structure M12L24 self-assembled complexes gives them potential to serve as catalyst carriers for enabling continuous chemical transformations. A stereoselective (allylic oxidation followed by Diels–Alder cyclization) with two intrinsically incompatible catalysts was demonstrated. Our system is advantageous in terms availability,...
We have shown that a type I phosphatidylinositol (PI) kinase activity is associated with the epidermal growth factor (EGF) receptor in mouse fibroblast cell line expressing human EGF receptors (NRHER5) and this increases dramatically upon treatment of cells physiologically relevant concentrations EGF. stimulated time-dependent increase receptor-associated PI measured immunoprecipitates. Activation was detected 15 min after addition EGF, it peaked between 1 2 hr. as low 10 pM maximal...
Nano- to micron-size reaction chamber arrays (femtolitre arrays) have facilitated the development of sensitive and quantitative biological assays, such as single-molecule enzymatic digital PCR ELISA. However, versatility femtolitre is limited reactions that occur in aqueous solutions. Here we report an arrayed lipid bilayer system (ALBiC) contains sub-million chambers, each sealed with a stable 4-μm-diameter membrane. When reconstituted limiting amount membrane transporter proteins...
Aortic dissection and intramural haematoma comprise an aortopathy involving separation of the aortic wall. Underlying mechanisms condition remain unclear. Here we show that granulocyte macrophage colony-stimulating factor (GM-CSF) is a triggering molecule for this condition. Transcription Krüppel-like 6 (KLF6)-myeloid-specific conditional deficient mice exhibit phenotype when subjected to inflammation. Mechanistically, KLF6 downregulates expression secretion GM-CSF. Administration...
"Molecular flasks" are well-defined supramolecular cages that can encapsulate one or more molecular guests within their cavities and, in so doing, change the physical properties and reactivities of guests. Although flasks powerful tools for manipulating matter on nanoscale, most them limited scope because size restrictions. Recently, however, increasingly large diverse have become available with enough space larger chemical systems such as polymers, nanoparticles, biomolecules. Here we...
Thermodynamically controlled platinum(II) spherical complexes were synthesized via temporary labilization of inert Pt(II)-pyridine bonds by the addition strong hydrogen-bond donor 2,2,2-trifluoroethanol (TFE), which weakens pyridine-metal interaction. The platinum complex was stably trapped after removal TFE and showed higher acid durability than its palladium counterpart.
The self-assembly of a cuboctahedral M12 L24 complex is traced by time-dependent NMR spectroscopy and mass spectrometry. metastable intermediate structures that exist during the process are not chaotic mixture numerous species, but instead geometrically restricted. Short-lived M8 L16 (D4d ) relatively long-lived M9 L18 (D3h fully characterized as major intermediates. Employing ligand with smaller bend angle (112°) allows these two species to be kinetically trapped more clearly observed...
For decades, chemists have strived to mimic the intricate design and diverse functions of naturally occurring systems through bioinspired synthesis programmable inorganic nanomaterials. The development thiol-capped gold nanoparticles (AuNPs) has driven advancement in this area; however, although versatile readily accessible, hybrid AuNPs are rarely atomically precise, which limits control over their surface topology therefore study complex structure–function relationships. Here, we present a...
Sesquiterpene scaffolds are the core backbones of many medicinally and industrially important natural products. A plethora sesquiterpene synthases, widely present in bacteria, fungi, plants, catalyze formation these intricate structures often with multiple stereocenters starting from linear farnesyl diphosphate substrates. Recent advances next-generation sequencing metabolomics technologies have greatly facilitated gene discovery for synthases. However, a major bottleneck limits biochemical...
Cationic palladium(II) ([Pd(MeCN)4](BF4)2) provides the first transition-metal-catalyzed method for electrophilic activation of electron-deficient 1,1-difluoro-1-alkenes, which allows their Friedel-Crafts-type cyclization with an intramolecular aryl group via a Wacker-type process. By using BF3.OEt2, was effected by catalytic amount palladium without its reoxidation.
Characterization of complex natural product mixtures to the absolute structural level their components often requires significant amounts starting materials and lengthy purification process, followed by arduous structure elucidation efforts. The crystalline sponge (CS) method has demonstrated utility in isolated organic compounds at miniscule quantities compared conventional methods. In this work, we developed a new CS-based workflow that greatly expedites in-depth analysis crude extracts....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTModulation of annexin II tetramer by tyrosine phosphorylationI. Hubaishy, P. G. Jones, J. Bjorge, C. Bellagamba, S. Fitzpatrick, D. Fujita, and M. WaismanCite this: Biochemistry 1995, 34, 44, 14527–14534Publication Date (Print):November 1, 1995Publication History Published online1 May 2002Published inissue 1 November 1995https://pubs.acs.org/doi/10.1021/bi00044a031https://doi.org/10.1021/bi00044a031research-articleACS PublicationsRequest reuse...
Many of the Src-like tyrosine kinases are thought to participate in multiprotein complexes that modulate transmembrane signalling through phosphorylation. We have used vitro binding studies employing bacterially expressed glutathione S-transferase-p56lck fusion proteins and cell extracts map regions on p56lck involved phosphatidylinositol 3'-kinase (PI3K). Deletions within SH3 domain abolished PI3K activity from T-cell lysates, whereas deletion SH2 caused only a slight reduction level bound...
Abstract Physiological and pathological functions of glycans are promoted through their clustering effects as exemplified by a series gangliosides, sialylated glycosphingolipids, which serve acceptors for bacterial toxins viruses. Furthermore, ganglioside GM1 clusters on neuronal cell membranes specifically interact with amyloidogenic proteins, triggering conformational transitions leading to neurodegeneration. Here we develop self‐assembled spherical complex that displays cluster the...
Antiplatelet drugs, frequently used for cardiovascular events with thrombotic involvement, are also regarded as possible promising agents primary prevention. The roles of P2Y12, an ADP receptor and the target thienopyridine antiplatelet not satisfactorily known in vascular wall. We investigated hypothesis that smooth muscle cell (VSMC) P2Y12 is involved wall inflammatory changes by upregulating monocyte chemoattractant protein-1 (MCP-1) promoting adhesion. at 10(-5) M induced a 3.6 ±...