The adverse effects of plastic on adult animal and human health have been receiving increasing attention. However, its potential toxicity to fetuses has not fully elucidated. Herein, biodistribution polystyrene (PS) particles was determined after the maternal mice were orally given PS micro- and/or nano-particles with without surface modifications during gestational days 1 17. results showed that microplastics (MPs) nanoparticles (NPs) mainly emerged in alimentary tract, brain, uterus,...

Because there is no curative treatment for spinal cord injury, establishing an ideal animal model important to identify injury mechanisms and develop therapies individuals suffering from injuries. In this article, we systematically review analyze various kinds of models assess their advantages disadvantages further studies.

Objective: Oridonin (Ori) is a diterpene compound that has multiple biological properties. Here, our study was conducted to observe the therapeutic effect of Ori on depression as well uncover mechanism. Methods: Lipopolysaccharide (LPS)-induced models were established both in C57BL/6 mice and primary astrocytes, which treated with Ori, autophagy agonist Rapamycin (Rap) inhibitor 3-Methyladenine (3-MA). The depressive-like behaviors assessed behavioral tests. Autophagy evaluated hippocampus...

Post-stroke depression is associated with reduced expression of brain-derived neurotrophic factor (BDNF). In this study, we evaluated whether BDNF overexpression affects depression-like behavior in a rat model post-stroke depression. The middle cerebral artery was occluded to produce focal ischemia. These rats were then subjected isolation-housing combined chronic unpredictable mild stress generate A gene lentiviral vector injected into the hippocampus. At 7 days after injection, western...

Chemotherapy leads to a loss of fertility and reproductive endocrine function, thereby increasing the risk premature ovarian failure (POF). Studies have suggested that transplantation mesenchymal stem cells could inhibit apoptosis in granulosa improve follicular development. In present study, effects human umbilical cord cell (UCMSC) on function after damage caused by chemotherapy mechanism underlying these were investigated. POF model rats obtained intraperitoneal injection...

Premature ovarian failure (POF) results from a number of disorders. The POF model is primarily based on chemotherapeutic injury, and hence not suitable for assessing the effects chronic stress function. Therefore, improved animal models are required to analyze reserve. feasibility unpredictable mild (CUMS) method establishing was examined. depressive behavior exhibited by rats evaluated with open field sucrose preference tests. Vaginal smears were obtained assessment estrous cycle. reserve...

The aim of this study was to investigate the effect vascular endothelial growth factor-C (VEGF-C) expression in tumor-associated macrophages (TAMs) on lymphatic microvessel density (LMVD) and cell proliferation (LECP) determine role VEGF-C lymphangiogenesis patients with breast cancer. Breast cancer tissue specimens confirmed by pathological analysis were obtained from 75 patients. Samples observed microscopy after immunohistochemical double‑staining. total number TAMs VEGF-C-positive...

Transplantation of human umbilical cord mesenchymal stem cells (hUC-MSCs) into spinal injury (SCI) may alleviate neuropathic pain and promote functional recovery. The underlying mechanism likely involves activation glial regulation inflammatory factors but requires further validation. SCI was induced in 16 ICR mice using an compression model, followed by injection lentiviral vector-mediated green fluorescent protein- (GFP-) labeled hUC-MSCs 1 week later. Behavioral tests, histological...

Imbalanced oxidative stress and antioxidant defense are involved in airway remodeling asthma. It has been demonstrated that Tetrandrine a potent role rheumatoid arthritis hypertension. However, the correlation between asthma is utterly blurry. This study aimed to investigate of on stress-mediated remolding.Chronic was established by ovalbumin (OVA) administration male Wistar rats. Histopathology determined HE staining. Immunofluorescence employed detect expression α-SMA Nrf-2. Level matrix...

To investigate the expression of vascular endothelial growth factor-c (VEGF-C) mRNA and microvessel density (MVD) in human esophageal squamous cell carcinoma (ESCC) its relationship with clinical significance.Specimens obtained from 43 patients undergoing surgical resection for ESCC were used this study. The VEGF-C was examined by situ hybridization. Tumor MVD determined immunohistochemically anti-CD31 antibody estimated image analysis. Ten sections adjacent normal mucosa also...

Summary One hundred and fifty piglets were randomly allotted to one of six treatments determine the effects olaquindox cyadox on growth intestinal immune response including number intraepithelial lymphocytes immunoglobulin A secreting cells (ASCs) during three‐week period. 2 × 3 factorial arrangement was employed with following factors: (1) Escherichia coli (O 139 :K 88 , 10 CFU) inoculation or control (2) no antimicrobials, 100 mg/kg in basal diet respectively. The antimicrobial...

Atherosclerosis is essentially the leading factor behind occurrences of cardiovascular diseases (CVDs)-associated incidents, while mitochondrial dysfunction also main cause atherosclerosis. The present study conducted a comparative analysis function-related indicators in cholesterol-induced vascular endothelial cells (VECs) from Mongolian gerbils, Sprague-Dawley (SD) rats and humans. It reported that inhibitory effect cholesterol treatment on viability gerbil VECs was markedly lower than...

BACKGROUND:This study assessed the effects and underlying molecular mechanisms of β-asarone on ischemic stroke model rats. MATERIAL AND METHODS:Ischemic was induced by middle cerebral artery occlusion (MCAO) in Before after modeling, cognitive function evaluated via fear conditioning test neurological deficit determined Longa Bederson scores. Following treatment with or nuclear factor erythroid 2-related 2 (Nrf2) inhibitor for 20 consecutive days, infarction detected TTC staining Cresyl...

BACKGROUND:Over-exposure to visible white light can cause retinal damage. Lotus seedpod proanthocyanidins (LSPCs) possess a variety of biological activities, including potent antioxidant and protective effects. Herein, this study observed whether LSPCs protect against exposure-induced MATERIAL AND METHODS:We randomly separated 40 Prague-Dawley rats into control group, injury model low-dose (50 mg/kg), medium-dose (100 high-dose mg/kg) groups. Light-induced damage models were established by...

This study aimed to investigate whether chronic unpredictable mild stress (CUMS) affects follicular development in ovaries through the nerve growth factor (NGF)/high affinity receptor, Tropomyosin-related kinase A (TrkA) mediated signaling pathway and reveal relationship between premature ovarian insufficiency (POI) development. In this experiment, a CUMS rat model was constructed. It found that serum estradiol (E2), anti-Mullerian hormone (AMH), gonadotropin-releasing (GnRH) levels...

BACKGROUND:Ulinastatin is a protease inhibitor derived from urine that has shown anti-inflammatory effects in human disease, including sepsis. Necrotizing enterocolitis (NEC) common gastrointestinal disease premature infants. Our aim was to explore the of ulinastatin on neonatal NEC rat model. MATERIAL AND METHODS:Forty-five rats were divided into 3 groups: normal control; NEC+sepsis-induced kidney injury (SIRS); NEC/SIRS+ulinastatin. The NEC/SIRS model induced by injection intraperitoneal...

Neurokinin-1 receptor (NK1R) is a high affinity Substance P (SP) and plays key role in visceral hypersensitivity irritable bowel syndrome (IBS). Early life stress significant risk factor IBS. The aim of the present study was to investigate influence neonatal maternal separation on expression distribution SP its along brain-gut axis maternally separated rat model with hypersensitivity. Male Sprague-Dawley rats, 2-21-day old, were randomly distributed into groups 3 h daily (MS) or non-handling...

Oldenlandia diffusa has been used to treat various cancers. Cytochrome P450, a drug metabolic enzyme, might be influenced by herbal medicine. Currently, the problem that remains is effective treatment in drug-drug interaction situation. Potential influences of were elucidated on CYP450 activities rats using cocktail method. Blood samples precipitated acetonitrile. Quantitative determination target test object was done ultra-performance liquid chromatography tandem mass spectrometry...

Core needle biopsy (CNB) may be used to diagnose early‑stage breast cancer, but it increase the risk of distant metastasis tumor cells. The aim present study was explore effect CNB on cancer. A total 30 BALB/c mice were divided into two groups, namely and non‑biopsy groups. biopsy‑related lung model (biopsy group) established by inoculation in mammary fat pad mouse cancer cell line 4T1 combined with CNB. Flow cytometry, quantitative polymerase chain reaction analysis, morphological as well...

In this study, a cocktail of probe drugs was used to assess whether lentinan could influence the activities rat enzymes CYP3A4, CYP2D6, CYP1A2, CYP2C19, and CYP2C9 in vivo.Fourteen days after intraperitoneal injection lentinan, rats were given an oral dose solution containing phenacetin, tolbutamide, omeprazole, metoprolol, midazolam. Then, we obtained blood specific durations for determination plasma concentration using UPLC-MS/MS. We also evaluated pharmacokinetic parameters DAS 2.0...

An experimental study examined the effect of basic fibroblast growth factor (bFGF) on healing corneal epithelial wounds in dogs. A wound was made one eye from each 40 dogs with a trephine (6 mm diameter). Four concentrations bFGF (0, 0.1, 0.5, and 1.0 μg/ml) were applied to affected eyes three times daily. Fluorescein staining used assess closure wounds. The morphological characteristics determined histological examination. rate significantly greater bFGF-treated group compared controls 1,...

The present study aimed to screen and find alkyl hydroperoxide reductase (AhpC) B cell dominant epitope of

Background: Immune-related genes possess promising prognostic potential in multiple cancer types. Here, we describe the development of an immune-related signature for predicting prostate recurrence. Methods: Prostate gene expression profiles 477 cases, as well accompanying follow-up information were downloaded from The Cancer Genome Atlas (TCGA) and GEO. samples divided into 3 groups immune sets significantly associated with prognosis identified by evaluating relationship between 1039...