A5022622352- Fibroblast Growth Factor Research
- Bone Tissue Engineering Materials
- TGF-β signaling in diseases
- Bone Metabolism and Diseases
- COVID-19 Clinical Research Studies
- Bone and Dental Protein Studies
- Collagen: Extraction and Characterization
- Corneal surgery and disorders
- Respiratory Support and Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Virus-based gene therapy research
- Connective tissue disorders research
- Epigenetics and DNA Methylation
- Long-Term Effects of COVID-19
- Neonatal Respiratory Health Research
- Corneal Surgery and Treatments
- Growth Hormone and Insulin-like Growth Factors
- Periodontal Regeneration and Treatments
- Dental Implant Techniques and Outcomes
- Ocular Surface and Contact Lens
- Viral Infectious Diseases and Gene Expression in Insects
- Legal processes and jurisprudence
- Autophagy in Disease and Therapy
- Latin American Legal and Economic Studies
- Congenital heart defects research
Masaryk University
2024-2025
St. Anne's University Hospital Brno
2024-2025
University Hospital Brno
2025
Universidad de Málaga
2014-2023
Instituto de Investigación Biomédica de Málaga
2015-2023
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2015-2023
Andalusian Centre for Nanomedicine and Biotechnology
2015-2022
Instituto de Salud Carlos III
2022
Hôpital de la Conception
2020
Junta de Andalucía
2016-2017
Keratoconjunctivitis sicca (KCS) or dry eye disease (DED) is an immune-mediated multifactorial disease, with high level of prevalence in humans and dogs. Our aim this study was to investigate the therapeutic effects allogeneic adipose-derived mesenchymal stromal cells (Ad-MSCs) implanted around lacrimal glands 12 dogs (24 eyes) KCS, which refractory current available treatments. Schirmer tear test (STT) ocular surface integrity were assessed at 0 (before treatment), 3, 6, 9 months after...
Most of our knowledge protein structure and function originates from experiments performed with purified proteins resuspended in dilute, buffered solutions. However, most crowded intracellular environments complex compositions. Significant efforts have been made to develop tools study their native cellular settings. Among these tools, in-cell NMR spectroscopy has the sole technique for characterizing space living cells at atomic resolution physiological temperature. Nevertheless, due...
Bone morphogenetic protein-2 (BMP-2) is widely used in orthopedic surgery and bone tissue engineering because of its strong osteogenic activity. However, BMP-2 treatments have several drawbacks many groups are actively exploring alternatives. Since BMP-6 has been demonstrated to be more osteoinductive, use, either alone or together with other growth factors, might an interesting option. In this work, we compared the effect BMP-2, BMP-6, insulin-like factor-1 (IGF-1), combination. Murine...
Primary cilium projects from cells to provide a communication platform with neighboring and the surrounding environment. This is ensured by selective entry of membrane receptors signaling molecules, producing fine-tuned effective responses extracellular cues. In this study, we focused on one family fibroblast growth factor (FGFRs), their residence within cilia, its role in FGFR signaling. We show that FGFR1 FGFR2, but not FGFR3 FGFR4, localize primary cilia developing mouse tissues vitro...
Cell communication systems based on polypeptide ligands use transmembrane receptors to transmit signals across the plasma membrane. In their biogenesis, depend endoplasmic reticulum (ER)-Golgi system for folding, maturation, transport and localization cell surface. ER stress, caused by protein overproduction misfolding, is a well-known pathology in neurodegeneration, cancer numerous other diseases. How stress affects via largely unknown. disease models of multiple myeloma, chronic...
Although many bone substitutes have been designed and produced, the development of tissue engineering products that mimic microstructural characteristics native remains challenging. It has shown pore orientation within collagen scaffolds influences matrix formation by endochondral route. In addition, unidirectional can limit growth blood vessels. However, a comparison between amount be formed in with different orientations addition to analyzing effect loading osteogenic proangiogenic factors...
Limbal stem cells (LSCs) are a quiescent cell population responsible for the renewal of corneal epithelium. Their deficiency is conjunctivization cornea that seen in different ocular pathologies, both humans and canine species. The species represents an interesting preclinical animal model surface pathologies. However, role LSCs physiological pathological conditions not well understood. Our objective was to characterize first time soluble factors proteomic profile secretome exosomes (cLSCs)....
Articular cartilage is an avascular tissue that lines the ends of bones in diarthrodial joints, serves as support, acts a shock absorber, and facilitates joint’s motion. It formed by chondrocytes immersed dense extracellular matrix (principally composed aggrecan linked to hyaluronic acid long chains). Damage this usually associated with traumatic injuries or age-associated processes often lead discomfort, pain disability our aging society. Currently, there are few surgical alternatives treat...
Transforming growth factor-beta (TGF-β) is involved in processes related to the differentiation and maturation of osteoprogenitor cells into osteoblasts. Rat bone marrow (BM) were cultured a collagen-gel containing 0.5% fetal bovine serum (FBS) for 10 days presence rhTGF (recombinant human TGF)-β1-F2, fusion protein engineered include high-affinity collagen-binding decapeptide derived from von Willebrand factor. Subsequently, moderately expanded medium with 10% FBS 4 treated short pulse...
In-cell NMR spectroscopy is the sole technique for characterizing protein structure, dynamics, and interactions in living human cells at atomic resolution. However, its applications have been restricted to asynchronous single-cell suspensions. We developed a strategy based on target inducible overexpression stably transfected cells, allowing acquisition of high-resolution in-cell spectra physiologically defined cellular states 3D tissue models.
Abstract Achondroplasia is the most common form of human dwarfism caused by mutations in FGFR3 receptor tyrosine kinase. Current therapy begins at two years age and improves longitudinal growth but does not address cranial malformations including midface hypoplasia foramen magnum stenosis, which lead to significant otolaryngeal neurologic compromise. A recent clinical trial found partial restoration defects with starting 3 months age, results are still inconclusive. The benefits...
Inflammation is a central pathogenic feature of the acute respiratory distress syndrome (ARDS) in COVID-19. Previous pathologies such as diabetes, autoimmune or cardiovascular diseases become risk factors for severe hyperinflammatory syndrome. A common among these subclinical presence cellular stress, finding that has gained attention after discovery BiP (GRP78), master regulator participates SARS-CoV-2 recognition. Here, we show serum levels are higher COVID-19 patients who present certain...
Alterations in the balance between skeletogenesis and adipogenesis is a pathogenic feature multiple skeletal disorders. Clinically, enhanced bone marrow adiposity bones impairs mobility increases fracture risk, reducing quality of life patients. The molecular mechanism that underlies not completely understood but alterations progenitor cells’ differentiation pathway plays key role. We recently demonstrated parathyroid hormone (PTH)/PTH-related peptide (PTHrP) control levels DEPTOR, an...
The presence of cartilage tissue in the embryonic and adult hearts different vertebrate species is a well-recorded fact. However, while neural crest has been historically considered as main source cardiac cartilage, recently reported results on wide connective potential epicardial lineage cells suggest they could also differentiate into chondrocytes. In this work, we describe formation clusters from proepicardial cells, both vivo vitro. Our findings report, for first time, progenitor...
In-cell NMR spectroscopy is the sole technique for characterizing protein structure, dynamics, and interactions in living human cells at atomic resolution. However, its applications have been restricted to asynchronous single-cell suspensions. We developed a strategy based on target inducible overexpression stably transfected cells, allowing acquisition of high-resolution in-cell spectra physiologically defined cellular states 3D tissue models.