A5000104264- Bone Metabolism and Diseases
- Estrogen and related hormone effects
- Mesenchymal stem cell research
- Bone health and osteoporosis research
- Bone health and treatments
- Connective tissue disorders research
- Neurological Disease Mechanisms and Treatments
- RNA regulation and disease
- Ubiquitin and proteasome pathways
- Biochemical Acid Research Studies
- TGF-β signaling in diseases
- Cancer, Hypoxia, and Metabolism
- Chemical Reactions and Isotopes
- Reproductive System and Pregnancy
- Bone and Joint Diseases
- Neurological Disorders and Treatments
- Autophagy in Disease and Therapy
- Fibroblast Growth Factor Research
- Mitochondrial Function and Pathology
- Bone fractures and treatments
- Protease and Inhibitor Mechanisms
- Pregnancy-related medical research
- Biochemical effects in animals
- Diet and metabolism studies
- Periodontal Regeneration and Treatments
University of California, Los Angeles
2023-2025
University of California Davis Medical Center
2015-2020
University of California, Davis
2016
Abstract In this study, we engineered mesenchymal stem cells (MSCs) to over-express basic fibroblast growth factor (bFGF) and evaluated its effects on fracture healing. Adipose-derived mouse MSCs were transduced express bFGF green fluorescence protein (ADSCbFGF-GFP). Closed-femoral fractures performed with osterix-mCherry reporter mice of both sexes. The received 3 × 105 ADSCs transfected control vector or via intramuscular injection within around the sites. Mice euthanized at days 7, 14, 35...
Abstract Mesenchymal stem cell (MSC) transplantation has been tested in animal and clinical fracture studies. We have developed a bone-seeking compound, LLP2A-Alendronate (LLP2A-Ale) that augments MSC homing to bone. The purpose of this study was determine whether treatment with LLP2A-Ale or combination MSCs would accelerate bone healing mouse closed model if the effects are sex dependent. A right mid-femur induced two-month-old osterix-mCherry (Osx-mCherry) male female reporter mice. mice...
Cell communication systems based on polypeptide ligands use transmembrane receptors to transmit signals across the plasma membrane. In their biogenesis, depend endoplasmic reticulum (ER)-Golgi system for folding, maturation, transport and localization cell surface. ER stress, caused by protein overproduction misfolding, is a well-known pathology in neurodegeneration, cancer numerous other diseases. How stress affects via largely unknown. disease models of multiple myeloma, chronic...
Abstract Activation and migration of endogenous mesenchymal stromal cells (MSCs) are critical for bone regeneration. Here, we report a combinational peptide screening strategy rapid discovery ligands that not only bind strongly to osteogenic progenitor (OPCs) but also stimulate cell Akt signaling in those OPCs. Two lead compounds discovered, YLL3 YLL8, both which increase osteoprogenitor differentiation vitro . When given normal or osteopenic mice, the mineral apposition rate, formation,...
One of the strongest predictors for osteoporosis is peak bone mass. Interventions to augment mass have yet be developed. β-Ecdysone (βEcd), a natural steroid-like compound produced by arthropods initiate metamorphosis, believed androgenic effects and so may used mass.The purpose this study was use both male female (1) gonadal-sufficient; (2) -insufficient mice investigate sex differences in terms development structure after βEcd administration.Two-month-old Swiss-Webster were randomized...
ABSTRACT The role of the progesterone receptor (PR) in regulation sexual dimorphism bone has yet to be determined. Here we utilized genetic fate mapping and Western blotting demonstrate age-dependent PR expression mouse femoral metaphysis diaphysis. To define sex-dependent osteoblast stage–specific effects on acquisition, selectively deleted at different stages differentiation. We found that when Prx1-Cre mice were crossed with floxed generate a mesenchymal stem cell (MSC) conditional KO...
Increasing peak bone mass is a promising strategy to prevent osteoporosis. A mouse model of global progesterone receptor (PR) ablation showed increased through sex-dependent mechanism. Cre-Lox recombination was used generate osteoprogenitor-specific PR inactivation, which recapitulated the high phenotype seen in knockout mode. In this work, we employed RNA sequencing analysis evaluate sex-independent and differences gene transcription osteoprogenitors wild-type conditional mice. deletion...
Osteogenesis imperfecta (OI), brittle bone disease, is a disorder characterized by fragility and increased fracture incidence. All forms of OI also feature short stature, implying an effect on endochondral ossification. Using the Aga2+/- mouse, which has mutation in type I collagen, we show affected growth plate primarily due to shortened proliferative zone. We used scRNAseq analysis tibial femoral tissues understand transcriptional consequences cell types. that perichondrial cells, express...
Abstract Bruck syndrome is an autosomal recessive form of osteogenesis imperfecta caused by biallelic variants in PLOD2 or FKBP10 and characterized joint contractures, bone fragility, short stature, scoliosis. encodes LH2, which hydroxylates type I collagen telopeptide lysines, a critical step for crosslinking. The Plod2 global knockout mouse model limited early embryonic lethality, thus, the role skeletogenesis not well understood. We generated novel line modeling variant identified two...