Abstract Embryo implantation and decidualization are crucial for a successful pregnancy. How the inflammatory response is regulated during these processes undefined. Pyroptosis an form of cell death mediated by gasdermin D (GSDMD). Through in vivo, cultured epithelial cells organoids, it shown that pyroptosis occurs at site. Compared with those on day 4 pseudopregnancy delayed implantation, pyroptosis‐related protein levels significantly increased pregnancy activated suggesting blastocysts...

This goal of this study was to examine immunohistochemical distribution leukemia inhibitory factor (LIF), LIF receptor (LIFR), and glycoprotein (gp) 130 in rhesus monkey uterus during the menstrual cycle early pregnancy. Pregnancy rate significantly reduced control group from 66.7% (12 18) 22.2% (4 with an injection goat anti-human recombinant immunoglobulin G into uterine lumen on Day 8 mainly localized glandular luminal epithelium. immunostaining luteal phase stronger than it proliferative...

Endometrial decidualization plays a pivotal role during early pregnancy. Compromised has been tightly associated with recurrent implantation failure (RIF). Primary cilium is an antenna-like sensory organelle and acts as signaling nexus to mediate Hh, Wnt, TGFβ, BMP, FGF, Notch signaling. However, whether primary involved in human still unknown. In this study, we found that cilia are present endometrial stromal cells. The ciliogenesis length increased by progesterone vitro vivo...

Maternal age is one of the most important factors affecting success maternal pregnancy. Uterine aging leading cause pregnancy failure in older women. However, how uterine affects receptivity and decidualization unclear. In this study, naturally aged one-year-old female mice were used to investigate effects on embryo implantation during early our we found abnormal mice. Aged mouse uterus indicates a decrease nuclear LAMIN A, an increase PRELAMIN A PROGERIN. uterus, double-stranded DNA (dsDNA)...

Primary cilia are antenna-like organelles that sense extracellular signals and function as signaling hubs essential for vertebrate development homeostasis. Decidualization is crucial pregnancy establishment maintenance in both humans mice. While primary present endometrial stromal cells, their role remains unknown. Here, we identify TMEM67, a key component of the ciliary transition zone, critical regulator mouse decidualization. Loss triggers RhoA-MLC2-dependent actomyosin contraction, which...

Acrylamide is a class 2A carcinogen with neurotoxicity and genotoxicity. In addition to industrial production, it ubiquitous in high-temperature heated high-carbohydrate foods. Numerous studies have confirmed the toxicity of ACR on reproduction. Implantation decidualization are crucial processes during establishment pregnancy rodents humans. However, its effect uterine implantation remains poorly understood. The objective this study elucidate mechanism by which affects mice. exposed daily...

Diethylstilbestrol (DES) is a synthetic non-steroidal estrogen, which was widely used to prevent preterm birth and abortion from the 1940s 1970s. DES can increase incidence of infertility, abnormal reproductive tract, autoimmune diseases. However, mechanism underlying on early pregnancy in mice unclear. This study evaluated effects mice, especially uterine receptivity decidualization. Newborn female were subcutaneously injected with 0.1 mg/kg DES, 1 or sesame oil as controls for 5...

Decidualization is necessary for the successful establishment of early pregnancy in rodents and humans. Disturbed decidualization results recurrent implantation failure, spontaneous abortion, preeclampsia. Tryptophan (Trp), one essential amino acids humans, has a positive effect on mammalian pregnancy. Interleukin 4-induced gene 1 (IL4I1) recently identified enzyme that can metabolize L-Trp to activate aryl hydrocarbon receptor (AHR). Although IDO1-catalyzed kynurenine (Kyn) from Trp been...

Abstract Signal transducer and activator of transcription (STATs) can be activated by many cytokines growth factors. Stat3, a member STAT family, is essential for embryonic development. Stat3 specifically during mouse embryo implantation. This study was to investigate the expression, activation, regulation in uterus early pregnancy, pseudopregnancy, delayed implantation, artificial decidualization, hormonal treatments using situ hybridization immunohistochemistry. There strong level...

Thyroid dysfunction during human pregnancy is closely related to serious outcome. However, the regulation and function of thyroid hormones early are largely unknown. We found that type II deiodinase, an enzyme converting T4 activated T3, highly expressed in mouse uterus on days 3 4 pregnancy. Once embryo implants into receptive uterus, III deiodinase (Dio3), a mainly paternally imprinted gene for inactivating significantly induced stromal cells accompanied by DNA hypermethylation intergenic...

Decidualization is essential for successful pregnancy in rodents and primates. Although L-Tryptophan its metabolites are mammalian pregnancy, the underlying mechanism poorly defined. We explored effects of tryptophan kynurenine on human vitro decidualization endometrial stromal cell line primary cells. Tryptophan significantly stimulates expression prolactin insulin growth factor binding protein 1, reliable markers decidualization. When cells treated with tryptophan, hydroxylase-1 remains...

The present study investigated the subcellular localization of inducible nitric oxide synthase (iNOS) during mouse oocyte meiotic maturation and fertilization using confocal microscopy, further studied roles iNOS-derived NO in by an iNOS-specific inhibitor aminoguanidine (AG) iNOS antibody microinjection. In germinal vesicle-stage oocytes, immunoreactivity was mainly localized vesicle. Shortly after vesicle breakdown, accumulated around condensed chromosomes. At metaphase I II, with...

(1) Background: Inflammatory responses are implicated in embryo implantation, decidualization, pregnancy maintenance and labor. Both implantation decidualization essential to successful rodents primates. S100A6 is involved inflammation, tumor development, apoptosis calcium homeostasis. strongly expressed mouse decidua, but the underlying mechanisms of how regulates poorly defined. (2) Methods: Mouse endometrial stromal epithelial cells isolated from day 4 pseudopregnant uteri....

Prostaglandin E 2 (PGE ) is shown to be essential for female reproduction. Cyclooxygenase (COX) a rate-limiting enzyme in prostaglandin synthesis from arachidonic acid and exists two isoforms: COX-1 COX-2. synthase (PGES) terminal prostanoid can catalyse the isomerization of COX product PGH PGE , including microsomal PGES-1 (mPGES-1), cytosolic PGES (cPGES) mPGES-2. This study examined protein expression COX-1, COX-2, mPGES-1, cPGES mPGES-2 preimplantation mouse embryos by...

Lipocalin-type prostaglandin D synthase (L-PGDS), a bifunctional protein, is expressed in the male reproductive organs of many species. However, expression and regulation L-PGDS rat are still uncertain. The present study investigated regionalization testis epididymis by situ hybridization immunohistochemistry under conditions sexual maturation, castration, ethylene dimethane sulfonate (EDS) treatments. In sexually mature rats, mRNA was weakly only testicular peritubular cells, whereas...

Glucocorticoids (GCs) are essential for mouse embryo implantation and decidualization. Excess GCs harmful 11β-Hydroxysteroid dehydrogenases type I II (Hsd11b1/Hsd11b2) main enzymes regulating local level of GCs. Hsd11b2 acts as the placental glucocorticoid barrier to protect fetus from excessive exposure. Although effects on placenta in late pregnancy have been extensively studied, these adrenal corticosteroids early far less well defined. Therefore, we examined expression, regulation...

Basigin is a member of the immunoglobulin superfamily and key molecule related to mouse blastocyst implantation. Whether preimplantation embryos express basigin mRNA still unknown. The aim this study was use quantitative competitive polymerase chain reaction assess quantitatively levels in oocyte embryos. detected all stages were 0.0606 ± 0.0282 oocyte, 0.0102 0.0036 zygote, 0.0007 0.0003 2-cell embryo, 0.0031 0.0017 4-cell 0.0084 0.0024 8-cell 0.0537 0.0121 morula 0.0392 0.0161 attomoles...

The aim of this study was to examine the effect alcohol on decidualization human endometrial stromal cells during early pregnancy.During in vitro decidualization, were treated with alcohol, 4-methylpyrazole hydrochloride (FPZ), inhibitor dehydrogenases (ADHs), and tetraethylthiuram disulfide (DSF), acetaldehyde (ALDHs), respectively. Cell viability examined. Apoptosis proliferation also evaluated.The findings showed that ADHs ALDHs up-regulated decidualization. After treatment, cell decidual...

Decidualization is required for the successful establishment of pregnancy in rodents and primates. Fatty acid desaturase 3 (Fads3) belongs to fatty family, which a crucial enzymes highly unsaturated biosynthesis. However, expression, regulation function Fads3 during early mice are still unknown. In this study, we examined mouse decidualization. The expression detected subluminal stromal cells at implantation site on day 5 pregnancy, but not inter-implantation pseudopregnant uteri. Compared...

Accumulating evidence shows that inflammation is essential for embryo implantation and decidualization. Histamine, a proinflammatory factor present in almost all mammalian tissues, synthesized through decarboxylating histidine by decarboxylase (HDC). Although histamine known to be decidualization, the underlying mechanism remains undefined. In study, had no obvious direct effects on vitro decidualization mice. However, differences HDC protein levels between day 4 of pregnancy...