A5088980124- RNA modifications and cancer
- Cancer-related gene regulation
- Growth Hormone and Insulin-like Growth Factors
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Endometriosis Research and Treatment
- Testicular diseases and treatments
- CRISPR and Genetic Engineering
- interferon and immune responses
- Ubiquitin and proteasome pathways
- Estrogen and related hormone effects
- Names, Identity, and Discrimination Research
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Law, AI, and Intellectual Property
- Privacy, Security, and Data Protection
- Reproductive System and Pregnancy
South China Agricultural University
2019-2024
Key Laboratory of Guangdong Province
2020
Embryo implantation, a crucial step in human reproduction, is tightly controlled by estrogen and progesterone (P 4 ) via receptor alpha (PGR), respectively. Here, we report that N 6 -methyladenosine (m A), the most abundant mRNA modification eukaryotes, plays an essential role embryo implantation through maintenance of P signaling. Conditional deletion methyltransferase-like 3 ( Mettl3 ), encoding m A writer METTL3, female reproductive tract using Cre mouse line with Pgr promoter Pgr-Cre...
Abstract Common polygenic diseases result from compounded risk contributed by multiple genetic variants, meaning that simultaneous correction or introduction of single nucleotide variants is required for disease modeling and gene therapy. Here, we show precise, efficient, multiplex base editing up to three target sites across 11 genes/loci in cynomolgus monkey embryos using CRISPR-based cytidine- adenine-base editors. Unbiased whole genome sequencing demonstrates high specificity embryos....
Glucocorticoids (GCs) are essential for mouse embryo implantation and decidualization. Excess GCs harmful 11β-Hydroxysteroid dehydrogenases type I II (Hsd11b1/Hsd11b2) main enzymes regulating local level of GCs. Hsd11b2 acts as the placental glucocorticoid barrier to protect fetus from excessive exposure. Although effects on placenta in late pregnancy have been extensively studied, these adrenal corticosteroids early far less well defined. Therefore, we examined expression, regulation...
Purpose: The aim of the present study was to explore role CHPF in non-small-cell lung cancer (NSCLC) and develop an shRNA vector-based therapy repress expression gene NSCLC cell lines. Methods: In this study, we used immunohistochemical staining verify tissue. Then, determined different lines with RT-PCR Western blotting. Specific knockdown CHPF. Celigo image cytometry, cycle analysis, flow cytometry assay were performed. Results: results showed that level higher tissues than normal tissues....
In most mammalian species, the testis descends from abdomen into scrotum during fetal or neonatal life. The failure of testicular descent, a pathological condition known as cryptorchidism, has long been subject scientific interest in wide range fields, including medicine, developmental biology, and evolutionary biology. this study, we analyzed global gene expression changes associated with experimental cryptorchidism mice by using RNA-seq. A total 453 differentially expressed genes were...