A5047350724- Spectroscopy Techniques in Biomedical and Chemical Research
- 3D Printing in Biomedical Research
- Spectroscopy and Chemometric Analyses
- Crystallography and molecular interactions
- Acne and Rosacea Treatments and Effects
- Pharmacological Effects of Natural Compounds
- Digital Imaging for Blood Diseases
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Advancements in Transdermal Drug Delivery
- Cell Image Analysis Techniques
- Nanofabrication and Lithography Techniques
- Sphingolipid Metabolism and Signaling
- Crystallization and Solubility Studies
- Lipid Membrane Structure and Behavior
- Pulmonary Hypertension Research and Treatments
- Cancer Cells and Metastasis
- Biosensors and Analytical Detection
- Cellular transport and secretion
- Additive Manufacturing and 3D Printing Technologies
- Protein Interaction Studies and Fluorescence Analysis
- Immune Cell Function and Interaction
- X-ray Diffraction in Crystallography
- Neuroinflammation and Neurodegeneration Mechanisms
- Monoclonal and Polyclonal Antibodies Research
Imperial College London
2023-2025
NIHR Imperial Biomedical Research Centre
2023
University of Michigan
2017-2019
Ann Arbor Center for Independent Living
2019
Michigan State University
2013
Confocal Raman spectral imaging (RSI) enables high-content, label-free visualization of a wide range molecules in biological specimens without sample preparation. However, reliable quantification the deconvoluted spectra is needed. Here we develop an integrated bioanalytical methodology, qRamanomics, to qualify RSI as tissue phantom calibrated tool for quantitative spatial chemotyping major classes biomolecules. Next, apply qRamanomics fixed 3D liver organoids generated from...
Supramolecular crystalline assembly constitutes a rational approach to bioengineer intracellular structures. Here, biocrystals of clofazimine (CFZ) that form in vivo within macrophages were measured have marked curvature. Isolated crystals, however, showed reduced curvature suggesting forces bend these drug crystals. Consistent with the ability elastically deform, inherent crystal structure principal molecular component biocrystals-the hydrochloride salt CFZ (CFZ-HCl)-has corrugated packing...
Abstract Two‐photon polymerization (2PP) 3D printing enables top–down biomaterial synthesis with nanoscale spatial resolution for de novo design of monodisperse injectable drug delivery systems. Spatiotemporal Controlled Release Inks Biocompatible polyEsters (SCRIBE) is a novel poly(lactic‐ co ‐glycolic acid)‐triacrylate resin family sub‐micron and tuneable hydrolysis that addresses the limitations current 2PP resins. SCRIBE direct hollow microparticles chemistry complex geometries...
Clofazimine (CFZ) is a broad spectrum antimycobacterial agent recommended by the World Health Organization as first line treatment for leprosy and second multidrug resistant tuberculosis. Oral administration of CFZ leads to red skin pigmentation side effect. Since weakly basic, phenazine dye, effect results from lipophilic partitioning circulating, free base (neutral) form into skin. Here, we developed stable biocompatible formulation CFZ-HCl microcrystals that mimics predominant drug...
Supramolecular crystalline assembly constitutes a rational approach to bioengineer intracellular structures. Here, biocrystals of clofazimine (CFZ) that form in vivo within macrophages were measured have marked curvature. Isolated crystals, however, showed reduced curvature suggesting forces bend these drug crystals. Consistent with the ability elastically deform, inherent crystal structure principal molecular component biocrystals—the hydrochloride salt CFZ (CFZ-HCl)—has corrugated packing...
Lithium (Li) is one of the currently prescribed drugs for bipolar disorders (BPDs) and has many neuro-regulatory immune-modulating properties. Because neuro-pathological diseases including BPDs have been associated with some level inflammation, Li's effect on inflammation may crucial consequences. Even though Li shown to pro- anti-inflammatory activities in different cell models, mechanisms involved these effects are not well understood. Moreover, presence activators Toll-like receptors...
Abstract Weakly basic, poorly soluble chemical agents could be exploited as building blocks for constructing sophisticated molecular devices inside the cells of living organisms. Here, using experimental and computational approaches, we probed relationship between biological mechanisms mediating lysosomal ion homeostasis self-assembly a weakly basic small molecule block (clofazimine) into functional, mechanopharmaceutical device (intracellular Crystal-Like Drug Inclusions – “CLDIs”) in...
Picogram-scale biomolecule standards enable calibration.
Abstract Epithelial‐to‐mesenchymal transition (EMT) is crucial for tumor progression, being linked to alterations in the extracellular matrix (ECM). Understanding ECM's role EMT can uncover new therapeutic targets, yet replicating these interactions vitro remains challenging. It shown that hybrid hydrogels of alginate (ALG) and cell‐derived decellularized ECM (dECM), with independently tunable composition stiffness, are useful 3D‐models explore impact breast on EMT. Soft RGD‐ALG (200 Pa),...
Two photon polymerization (2PP) 3D printing enables top-down biomaterial synthesis with nanoscale spatial resolution for de novo design of monodisperse injectable drug delivery systems. To address the limitations current 2PP resins, we developed Spatiotemporal Controlled Release Inks Biocompatible polyEsters (SCRIBE), a novel poly(lactic-co-glycolic acid)-triacrylate resin family sub-micron and tuneable hydrolysis. SCRIBE direct hollow microparticles chemistry complex geometries inaccessible...