A5003146771- Immune Response and Inflammation
- Erythrocyte Function and Pathophysiology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune cells in cancer
- Corneal Surgery and Treatments
- Antimicrobial Peptides and Activities
- Sphingolipid Metabolism and Signaling
- Drug Transport and Resistance Mechanisms
- Inflammasome and immune disorders
- Medical and Biological Ozone Research
- Advancements in Transdermal Drug Delivery
- Autoimmune and Inflammatory Disorders
- Ocular Surface and Contact Lens
- Inflammatory Bowel Disease
- Tuberculosis Research and Epidemiology
- Advanced Glycation End Products research
- Ocular Diseases and Behçet’s Syndrome
- Pediatric health and respiratory diseases
- HIV Research and Treatment
- Drug-Induced Hepatotoxicity and Protection
- S100 Proteins and Annexins
- Advanced Drug Delivery Systems
- Ocular Infections and Treatments
- Bee Products Chemical Analysis
- Pharmacological Effects of Natural Compounds
The Royal Wolverhampton NHS Trust
2024
University of Michigan
2015-2018
Wayne State University
2009-2013
Kresge Eye Institute
2009-2013
Clofazimine (CFZ) is an FDA-approved leprostatic and anti-inflammatory drug that massively accumulates in macrophages, forming insoluble, intracellular crystal-like inclusions (CLDIs) during long-term oral dosing. Interestingly, when added to cells vitro, soluble CFZ cytotoxic because it depolarizes mitochondria induces apoptosis. Accordingly, we hypothesized that, vivo, macrophages detoxify by sequestering CLDIs. To test this hypothesis, CLDIs of CFZ-treated mice were biochemically isolated...
Clofazimine (CFZ) is a poorly soluble antibiotic and anti-inflammatory drug indicated for the treatment of leprosy. In spite its therapeutic value, CFZ therapy accompanied by formation biocrystals that accumulate within resident tissue macrophages, without obvious toxicological manifestations. Therefore, to specifically elucidate off-target consequences bioaccumulation in we compared level inflammasome activation CFZ-accumulating organs (spleen, liver lung) mice after 2 8 weeks when exists...
We previously showed that pre-exposure of the cornea to Toll-like receptor (TLR)5 ligand flagellin induces strong protective innate defense against microbial pathogens and hypothesized modulates gene expression at transcriptional levels. Thus, we sought determine role one transcription factor, interferon regulatory factor (IRF1), its target CXCL10 therein.Superarray was used identify factors differentially expressed in Pseudomonas aeruginosa-challenged human corneal epithelial cells (CECs)...
Abstract Clofazimine (CFZ) is an optically active, red‐colored chemotherapeutic agent that FDA approved for the treatment of leprosy and on World Health Organization's list essential medications. Interestingly, CFZ massively accumulates in macrophages where it forms crystal‐like drug inclusions (CLDIs) after oral administration animals humans. The analysis fluorescence spectra CLDIs formed by resident tissue revealed CFZ, when accumulated as CLDIs, undergoes a red shift excitation (from Ex:...
Following prolonged administration, certain orally bioavailable but poorly soluble small molecule drugs are prone to precipitate out and form crystal-like drug inclusions (CLDIs) within the cells of living organisms. In this research, we present a quantitative multi-parameter imaging platform for measuring fluorescence polarization diattenuation signals harboring intracellular CLDIs. To validate system, FDA-approved clofazimine (CFZ) was used as model compound. Our results demonstrated that...