A5077450097- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
- HIV Research and Treatment
- CAR-T cell therapy research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- COVID-19 Impact on Reproduction
- HIV/AIDS Research and Interventions
- IL-33, ST2, and ILC Pathways
- Cell Adhesion Molecules Research
- Hydrogen's biological and therapeutic effects
- Immune cells in cancer
- Cytomegalovirus and herpesvirus research
- Single-cell and spatial transcriptomics
- Gene Regulatory Network Analysis
- HIV-related health complications and treatments
- COVID-19 and healthcare impacts
- Cell Image Analysis Techniques
- Autophagy in Disease and Therapy
- Reproductive Health and Technologies
- HIV/AIDS drug development and treatment
- Immune Response and Inflammation
IRCCS Humanitas Research Hospital
2021-2024
Humanitas University
2017-2020
Istituti di Ricovero e Cura a Carattere Scientifico
2020
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani
2012-2017
Adoptive T cell transfer (ACT) immunotherapy benefits from early differentiated stem memory (Tscm) cells capable of persisting in the long term and generating potent antitumor effectors. Due to their paucity ex vivo, Tscm can be derived naive precursors, but molecular signals at basis generation are ill-defined. We found that less human circulating CD8+ display substantial antioxidant capacity vivo compared with more central effector cells. Limiting ROS metabolism antioxidants during...
Flow cytometry (FCM) can investigate dozens of parameters from millions cells and hundreds specimens in a short time at reasonable cost, but the amount data that is generated considerable. Computational approaches are useful to identify novel subpopulations molecular biomarkers, generally require deep expertize bioinformatics use different platforms. To overcome these limitations, we introduce CRUSTY, an interactive, user-friendly webtool incorporating most popular algorithms for FCM...
Abstract In mice, the ability of naive T (TN) cells to mount an effector response correlates with TCR sensitivity for self-derived Ags, which can be quantified indirectly by measuring surface expression levels CD5. Equivalent findings have not been reported previously in humans. We identified two discrete subsets human CD8+ TN cells, defined absence or presence chemokine receptor CXCR3. The more abundant CXCR3+ cell subset displayed effector-like transcriptional profile and expressed TCRs...
NK cells are endowed with tumor killing ability, nevertheless most cancers impair cell functionality, and cell-based therapies have limited efficacy in solid tumors. How render dysfunctional is unclear, overcoming resistance an important immune-therapeutic aim. Here, we identify autophagy as a central regulator of anti-tumor function. Analysis differentially expressed genes tumor-infiltrating versus non-tumor from our previously published scRNA-seq data advanced human prostate cancer shows...
Abstract Human T memory stem (T SCM ) cells with superior persistence capacity and effector functions are emerging as important players in the maintenance of long‐lived T‐cell thus considered an attractive population to be used adoptive transfer‐based immunotherapy cancer. However, molecular signals regulating their generation remain poorly defined. Here we show that curtailed receptor stimulation curbs human CD8 + differentiation allows CD45RO – CD45RA CCR7 CD27 CD95 ‐phenotype from highly...
Background: Immunological nonresponse represents the Achilles heel in combination antiretroviral therapy (cART) effectiveness, and increases risk of clinical events death. CD8 T cells play a crucial role controlling HIV replication, polyfunctional HIV-specific have been associated with nonprogressive infection. However, possible predicting posttreatment immune reconstitution has not yet explored. The aim this study was to identify functional markers predictive immunological response cART...
Alteration of γδ T-cell distribution and function in peripheral blood is among the earliest defects during HIV-infection. We asked whether polyfunctional response could also be affected, how this impairment associated to CD4 count. To aim, we performed a cross-sectional study on HIV-infected individuals. In order evaluate polyfunctional-Vγ9Vδ2 after phosphoantigen-stimulation, assessed cytokine/chemokine production cytotoxicity by flow-cytometry HAART-treated-HIV+ persons healthy-donors....
SARS-CoV-2, the virus behind COVID-19 pandemic, affects multiple organs, including male reproductive system. The tract expresses ACE-2, primary viral entry receptor, in spermatogonia, Leydig, and Sertoli cells. While infections can harm fertility through cytokine storms, effects of SARS-CoV-2 on are still unclear. Thus, this study aimed to examine impact tract. Semen samples from 20 patients, collected 3 months post-SARS-CoV-2 infection, revealed presence ACE2 TMPRSS2 receptors both...