A5008047821- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Cellular transport and secretion
- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- Lysosomal Storage Disorders Research
- Advanced Proteomics Techniques and Applications
- Mosquito-borne diseases and control
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
University of Wisconsin–Madison
2023-2025
Therapeutic RNAs are routinely modified during their synthesis to ensure proper drug uptake, stability, and efficacy. Phosphorothioate (PS) RNA, molecules in which one or more backbone phosphates with a sulfur atom place of standard nonbridging oxygen, is the most common modifications because ease pharmacokinetic benefits. Quality assessment RNA synthesis, including modification incorporation, essential for selectivity performance, synthetic nature PS linkage incorporation often reveals...
As lipidomics experiments increase in scale and complexity, data processing tools must support workflows for new liquid chromatography–mass spectrometry (LC-MS) methods while simultaneously supporting quality controls to maximize the confidence lipid identifications. LipiDex 2 improves algorithms from 1 introduces spectral matching peak annotation functions, with improvements speed user-friendliness. In silico library generation now supports tandem mass (MSn) tree-based fragmentation...
Lysosomal storage diseases (LSDs) comprise ~50 monogenic disorders marked by the buildup of cellular material in lysosomes, yet systematic global molecular phenotyping proteins and lipids is lacking. We present a nanoflow-based multiomic single-shot technology (nMOST) workflow that quantifies HeLa cell proteomes lipidomes from over two dozen LSD mutants. Global cross-correlation analysis between identified autophagy defects, notably accumulation ferritinophagy substrates receptors,...
In mass spectrometry-based lipidomics, complex lipid mixtures undergo chromatographic separation, are ionized, and detected using tandem MS (MSn) to simultaneously quantify structurally characterize eluting species. The reported structural granularity of these identified lipids is strongly reliant on the analytical techniques leveraged in a study. For example, identifications from traditional collisionally activated data-dependent acquisition experiments often at either species level or...
Abstract Type 2 diabetes is a challenge in modern healthcare, and animal models are necessary to identify underlying mechanisms. The Nile rat ( Arvicanthis niloticus ) develops diet-induced rapidly on conventional rodent chow diet without genetic or chemical manipulation. Unlike common laboratory models, the outbred model diurnal has wide range of overt onset progression patterns both sexes, better mimicking heterogeneous diabetic phenotype humans. While fasted blood glucose historically...
SUMMARY Lysosomal storage diseases (LSDs) comprised ∼50 monogenic characterized by the accumulation of cellular material in lysosomes and associated defects lysosomal function, but systematic molecular phenotyping is lacking. Here, we develop a nanoflow-based multi-omic single-shot technology (nMOST) workflow allowing simultaneously quantify HeLa cell proteomes lipidomes from more than two dozen LSD mutants, revealing diverse phenotypes. Defects delivery ferritin its autophagic receptor...
Abstract Type 2 diabetes is a challenge in modern healthcare, and animal models are necessary to identify underlying mechanisms, where we can achieve much better environmental control than what practical human studies. The Nile rat ( Arvicanthis niloticus ) develops diet-induced rapidly on conventional rodent chow diet without genetic or chemical manipulation. Unlike common laboratory models, the outbred model diurnal progress advanced diabetic complications, mimicking condition. Some...