A5070738917- Adipose Tissue and Metabolism
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Metabolomics and Mass Spectrometry Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Mitochondrial Function and Pathology
- Cellular transport and secretion
- Bioenergy crop production and management
- Autophagy in Disease and Therapy
- Fungal and yeast genetics research
- Endoplasmic Reticulum Stress and Disease
- Lysosomal Storage Disorders Research
- Lipid metabolism and biosynthesis
- Advanced Proteomics Techniques and Applications
- Glycosylation and Glycoproteins Research
- Mosquito-borne diseases and control
- Colorectal and Anal Carcinomas
- RNA modifications and cancer
- Acute Myeloid Leukemia Research
- Bacteriophages and microbial interactions
- Viral-associated cancers and disorders
- Galectins and Cancer Biology
- Genetics, Aging, and Longevity in Model Organisms
- High Altitude and Hypoxia
- Muscle metabolism and nutrition
University of Wisconsin–Madison
2017-2025
Great Lakes Bioenergy Research Center
2022-2025
Multi-omics analysis is a powerful and increasingly utilized approach to gain insight into complex biological systems. One major hindrance with multi-omics, however, the lengthy wasteful sample preparation process. Preparing samples for mass spectrometry (MS)-based multi-omics involves extraction of metabolites lipids organic solvents, precipitation proteins, overnight digestion proteins. These existing workflows are disparate laborious. Here, we present simple, efficient, unified prepare...
Exposure of adipocytes to 'cool' temperatures often found in the periphery body induces expression Stearoyl-CoA Desaturase-1 (Scd1), an enzyme that converts saturated fatty acids monounsaturated acids. In this study, we employed Scd1 knockout cells and mouse models, along with pharmacological inhibition, investigate further roles adipocytes. Our study reveals production lipids by is necessary for fusion autophagosomes lysosomes a Scd1-deficiency, accumulate. addition, Scd1-deficiency impairs...
Mass spectrometry-based large-scale multi-omics research has proven to be powerful in answering biological questions; nonetheless, it faces many challenges from sample preparation downstream data integration. To efficiently extract biomolecules of different physicochemical properties, various type needs specific tailoring, especially difficult ones, such as Caenorhabditis elegans. In this study, we sought develop a method starting with single set ofC. elegans samples save time, minimize...
One-fifth of human proteins are N-glycosylated in the endoplasmic reticulum (ER) by two oligosaccharyltransferases, OST-A and OST-B. Contrary to prevailing view N-glycosylation as a housekeeping function, we identified an ER pathway that modulates activity OST-A. Genetic analyses linked HSP90B1, chaperone for membrane receptors, CCDC134, luminal protein. During its translocation into ER, N-terminal peptide HSP90B1 templates assembly translocon complex containing CCDC134 protects during...
Abstract Biofuels derived from renewable and sustainable lignocellulosic biomass, such as switchgrass, offer a promising means to limit greenhouse gas emissions. However, switchgrass grown under drought conditions contains high levels of chemical compounds that inhibit microbial conversion biofuels. Fermentation hydrolysates by engineered Saccharomyces cerevisiae Zymomonas mobilis generates less ethanol than fermentation hydrolyzed an average rainfall year. Here, we demonstrate this...
Lysosomal storage diseases (LSDs) comprise ~50 monogenic disorders marked by the buildup of cellular material in lysosomes, yet systematic global molecular phenotyping proteins and lipids is lacking. We present a nanoflow-based multiomic single-shot technology (nMOST) workflow that quantifies HeLa cell proteomes lipidomes from over two dozen LSD mutants. Global cross-correlation analysis between identified autophagy defects, notably accumulation ferritinophagy substrates receptors,...
Abstract Thermodynamically constrained reactions and pathways are hypothesized to impose greater protein demands on cells, requiring higher enzyme amounts sustain a given flux compared those with stronger thermodynamics. To test this, we quantified the absolute concentrations of glycolytic enzymes in three bacterial species — Zymomonas mobilis , Escherichia coli Clostridium thermocellum which employ distinct varying thermodynamic driving forces. By integrating concentration data...
ABSTRACT One-fifth of human proteins are N-glycosylated in the endoplasmic reticulum (ER) by two oligosaccharyltransferases, OST-A and OST-B. Contrary to prevailing view N-glycosylation as a housekeeping function, we identified an ER pathway that modulates activity OST-A. Genetic analyses linked HSP90B1, chaperone for membrane receptors, CCCD134, protein identify first specificity factor N-glycosylation. During its translocation into ER, N-terminal peptide HSP90B1 functions pseudosubstrate...
Abstract Background Developing economically viable pathways to produce renewable energy has become an important research theme in recent years. Lignocellulosic biomass is a promising feedstock that can be converted into second-generation biofuels and bioproducts. Global warming adversely affected climate change causing many environmental changes have impacted earth surface temperature rainfall patterns. Recent shown growth conditions altered the composition of drought-stressed switchgrass...
Organisms have evolved elaborate physiological pathways that regulate growth, proliferation, metabolism, and stress response. These must be properly coordinated to elicit the appropriate response an ever-changing environment. While individual been well studied in a variety of model systems, there remains much uncover about how are integrated produce systemic changes cell, especially dynamic conditions. We previously showed deletion Protein Kinase A (PKA) regulatory subunit BCY1 can decouple...
Abstract Type 2 diabetes is a challenge in modern healthcare, and animal models are necessary to identify underlying mechanisms. The Nile rat ( Arvicanthis niloticus ) develops diet-induced rapidly on conventional rodent chow diet without genetic or chemical manipulation. Unlike common laboratory models, the outbred model diurnal has wide range of overt onset progression patterns both sexes, better mimicking heterogeneous diabetic phenotype humans. While fasted blood glucose historically...
Reduced skeletal muscle mass and oxidative capacity coexist in patients with pulmonary emphysema are independently associated higher mortality. If reduced cellular respiration contributes to atrophy that setting remains unknown. Using a mouse genetically induced recapitulates dysfunction, we found activity of succinate dehydrogenase (SDH) is hallmark its myopathic changes. We generated an inducible, muscle-specific SDH knockout demonstrates lower mitochondrial oxygen consumption, myofiber...
The oxidant hydrogen peroxide serves as a signaling molecule that alters many aspects of cardiovascular functions. Recent studies suggest cytoglobin - hemoglobin expressed in the vasculature may promote electron transfer reactions with proposed functions decomposition. Here, we determined extent to which regulates intracellular and established mechanisms. We found decreased hyperoxidation peroxiredoxins maintained activity peroxiredoxin 2 following challenge exogenous peroxide. Cytoglobin...
SUMMARY Lysosomal storage diseases (LSDs) comprised ∼50 monogenic characterized by the accumulation of cellular material in lysosomes and associated defects lysosomal function, but systematic molecular phenotyping is lacking. Here, we develop a nanoflow-based multi-omic single-shot technology (nMOST) workflow allowing simultaneously quantify HeLa cell proteomes lipidomes from more than two dozen LSD mutants, revealing diverse phenotypes. Defects delivery ferritin its autophagic receptor...
Abstract Type 2 diabetes is a challenge in modern healthcare, and animal models are necessary to identify underlying mechanisms, where we can achieve much better environmental control than what practical human studies. The Nile rat ( Arvicanthis niloticus ) develops diet-induced rapidly on conventional rodent chow diet without genetic or chemical manipulation. Unlike common laboratory models, the outbred model diurnal progress advanced diabetic complications, mimicking condition. Some...
ABSTRACT Exposure of adipocytes to ‘cool’ temperatures often found in the periphery body induces expression Stearoyl-CoA Desaturase-1 (SCD1), an enzyme that converts saturated fatty acids monounsaturated acids. In this study, we employed Scd1 knockout cells and mouse models, along with pharmacological SCD1 inhibition, investigate further roles adipocytes. Our study reveals production lipids by is necessary for fusion autophagosomes lysosomes a SCD1-deficiency, accumulate. addition,...
ABSTRACT With age, people tend to accumulate body fat and reduce energy expenditure 1 . Brown (BAT) beige adipose tissue dissipate heat increase via the activity of uncoupling protein UCP1 other thermogenic futile cycles 2,3 The brown depots inversely correlates with BMI age 4–11 , suggesting that promoting thermogenesis may be an effective approach for combating age-related metabolic disease 12–15 Heme is enzyme cofactor signaling molecule we recently showed regulate BAT function 16 Here,...
Abstract Organisms have evolved elaborate physiological pathways that regulate growth, proliferation, metabolism, and stress response. These must be properly coordinated to elicit the appropriate response an ever-changing environment. While individual been well studied in a variety of model systems, there remains much uncover about how are integrated produce systemic changes cell, especially dynamic conditions. We previously showed deletion Protein Kinase A (PKA) regulatory subunit BCY1 can...
Lipids are a class of molecules that have roles in energy storage, plasma membrane integrity, and signaling events. To gain more understanding the functions lipids play biology, researchers employ discovery analytical approaches, such as mass spectrometry (MS)-based lipidomics. The main objective this protocol is to provide directive on how extract from plasma, cells, tissue, purified organelles for analysis by liquid chromatography (LC)-MS. This will typically yield quantitative data than...