A5061301908- Biochemical and Molecular Research
- DNA Repair Mechanisms
- Enzyme Structure and Function
- PARP inhibition in cancer therapy
- RNA and protein synthesis mechanisms
- Chronic Lymphocytic Leukemia Research
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- Folate and B Vitamins Research
- Geomagnetism and Paleomagnetism Studies
- Protein Kinase Regulation and GTPase Signaling
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Metabolism and Genetic Disorders
- Magnetic and Electromagnetic Effects
- Chemical Reactions and Isotopes
- Glycosylation and Glycoproteins Research
- Heat shock proteins research
- Hearing, Cochlea, Tinnitus, Genetics
- Neuroscience and Neuropharmacology Research
- Cancer Immunotherapy and Biomarkers
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
CEA Cadarache
2015-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2012-2024
Institut de Biosciences et Biotechnologies
2015-2024
Centre National de la Recherche Scientifique
2007-2024
Aix-Marseille Université
2009-2024
Centre National pour la Recherche Scientifique et Technique (CNRST)
2023
Architecture et Fonction des Macromolécules Biologiques
2007-2018
Laboratoire de Neurosciences Intégratives et Adaptatives
2016
Institute of Environmental Biology and Biotechnology
2015
Karolinska Institutet
2009-2014
Background Steroidogenic acute regulatory (StAR) protein related lipid transfer (START) domains are small globular modules that form a cavity where lipids and hormones bind. These can transport ligands to facilitate exchange between biological membranes, they have been postulated modulate the activity of other in response ligand binding. More than dozen human genes encode START domains, several them implicated disease. Principal Findings We report crystal structures STARD1, STARD5, STARD13...
Magnetotactic bacteria consist of a group taxonomically, physiologically and morphologically diverse prokaryotes, with the singular ability to align geomagnetic field lines, phenomenon referred as magnetotaxis. This magnetotactic property is due presence iron-rich crystals embedded in lipidic vesicles forming an organelle called magnetosome. Magnetosomes are composed single-magnetic-domain nanocrystals magnetite (Fe3O4) or greigite (Fe3S4) biological membranes, thereby prokaryotic organelle....
Formate dehydrogenases (FDHs) are of interest as they natural catalysts that sequester atmospheric CO2, generating reduced carbon compounds with possible uses fuel. FDHs activity in Escherichia coli strictly requires the sulphurtransferase EcFdhD, which likely transfers sulphur from IscS to molybdenum cofactor (Mo-bisPGD) FDHs. Here we show EcFdhD binds Mo-bisPGD vivo and has submicromolar affinity for GDP—used a surrogate cofactor's nucleotide moieties. The crystal structure complex GDP...
Phages infecting Lactococcus lactis, a Gram-positive bacterium, are recurrent problem in the dairy industry. Despite their economical importance, knowledge on these phages, belonging mostly to Siphoviridae, lags behind that accumulated for members of Myoviridae. The three-dimensional structures receptor-binding proteins (RBP) three lactococcal phages have been determined recently, illustrating modular assembly and assigning nature bacterial receptor. These RBPs attached baseplate, large...
Homeostatic plasticity, the ability of neurons to maintain their averaged activity constant around a set point value, is thought account for central hyperactivity after hearing loss. Here, we investigated putative role GABAergic neurotransmission in this mechanism noise-induced loss larger than 50 dB high frequencies guinea pigs. The effect inhibition linked normal functioning K + -Cl- co-transporter isoform 2 (KCC2) which maintains low intracellular concentration chloride. expression...
The early B-cell factor (EBF) transcription factors are central regulators of development in several organs and tissues. This protein family shows low sequence similarity to other families, which is why structural information for the functional domains these proteins crucial understand their biochemical features. We have used a modular approach determine crystal structures structured EBF family. DNA binding domain reveals striking resemblance Rel homology superfamily but contains unique zinc...
While many studies have been devoted to investigating the homeostatic plasticity triggered by cochlear hearing loss, cellular and molecular mechanisms involved in these central changes remain elusive. In present study, we investigated possibility of reactive neurogenesis after unilateral nerve section nucleus (CN) cats. We found a strong cell proliferation all CN sub-divisions ipsilateral lesion. Most newly generated cells survive up 1 month deafferentation nuclei (except dorsal CN) give...
Methionine (Met) is prone to oxidation and can be converted Met sulfoxide (MetO), which exists as R- S-diastereomers. MetO reduced back by the ubiquitous methionine reductase (Msr) enzymes. Canonical MsrA MsrB were shown absolutely stereospecific for reduction of S-diastereomer R-diastereomer, respectively. Recently, a new enzymatic system, MsrQ/MsrP conserved in all gram-negative bacteria, was identified key actor oxidized periplasmic proteins. The haem-binding membrane protein MsrQ...
Abstract Exposure to harmful conditions such as radiation and desiccation induce oxidative stress DNA damage. In radiation-resistant Deinococcus bacteria, the radiation/desiccation response is controlled by two proteins: XRE family transcriptional repressor DdrO COG2856 metalloprotease IrrE. The latter cleaves inactivates DdrO. Here, we report biochemical characterization crystal structure of DdrO, which first a protein targeted protein. composed domains that fold independently are separated...
We report here the characterization of nonstructural protein ORF12 virulent lactococcal phage p2, which belongs to Siphoviridae family. was produced as a soluble protein, forms large oligomers (6- 15-mers) in solution. Using anti-ORF12 antibodies, we have confirmed that is not found virion structure but detected second half lytic cycle, indicating it late-expressed protein. The ORF12, solved by single anomalous diffraction and refined at 2.9-A resolution, revealed previously unknown fold...
ABSTRACT Lactococcus lactis , a gram-positive bacterium widely used by the dairy industry to manufacture cheeses, is subject infection diverse population of virulent phages. We have previously determined structures three receptor binding proteins (RBPs) from lactococcal phages TP901-1, p2, and bIL170, each them having distinct host range. Virulent p2 bIL170 are classified within 936 group, while temperate phage TP901-1 member genetically P335 polythetic group. These RBPs comprise domains:...
Strict management of intracellular heme pools, which are both toxic and beneficial, is crucial for bacterial survival during infection. The human pathogen
In proteins, methionine (Met) can be oxidized into Met sulfoxide (MetO). The ubiquitous reductases (Msr) A and B are thiol-oxidoreductases reducing MetO. Reversible oxidation has a wide range of consequences, from protection against oxidative stress to fine-tuned regulation protein functions. Bacteria distinguish themselves by the production molybdenum-containing enzymes MetO, such as periplasmic MsrP which protects proteins during acute stress. versatile dimethyl (DMSO) were shown reduce...
Magnetotactic bacteria (MTB) can swim along Earth's magnetic field lines, thanks to the alignment of dedicated cytoplasmic organelles. These organelles, termed magnetosomes, are proteolipidic vesicles filled by a 35-120 nm crystal either magnetite or greigite. The formation and magnetosomes mediated group specific genes, mam encoding magnetosome-associated proteins. whole process magnetosome biogenesis be divided into four sequential steps; (i) membrane invagination, (ii) alignment, (iii)...
Abstract The reaction centre-light harvesting 1 (RC-LH1) complex is essential for converting light into proton motive force in photosynthetic bacteria. RC-LH1 a monomer most purple bacteria, but Rhodobacter species, it dimer. Its assembly depends on an accessory polypeptide (PufX) and, ultimately, growth. To date, knowledge the RC-LH1-PufX structure, where dimer has two incomplete ‘C’-shaped antenna rings surrounding RC, mainly limited to model organism sphaeroides . Here we present...
Abstract Conserved proteins across oxygenic phototrophic organisms are important players in photosynthetic acclimation, yet many remain poorly or completely uncharacterized. In the green alga Chlamydomonas reinhardtii we identified Cre15.g636050, previously annotated as CPLD31 , that encodes such an uncharacterized, conserved, thylakoid lumenal protein belonging to “TPM” domain family (PF04536). TPM refers a fold named after three founding of this family, TLP18.3, Psb32, and MOLO-1, with...