A5061596947- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Animal Behavior and Reproduction
- Olfactory and Sensory Function Studies
- Skin and Cellular Biology Research
- Developmental Biology and Gene Regulation
- Invertebrate Immune Response Mechanisms
- Microtubule and mitosis dynamics
- Axon Guidance and Neuronal Signaling
- Wnt/β-catenin signaling in development and cancer
- Cellular Mechanics and Interactions
- Circadian rhythm and melatonin
- Acupuncture Treatment Research Studies
- Moyamoya disease diagnosis and treatment
- Hedgehog Signaling Pathway Studies
- Physiological and biochemical adaptations
- Biochemical Acid Research Studies
- Intracranial Aneurysms: Treatment and Complications
- Hippo pathway signaling and YAP/TAZ
- Oral health in cancer treatment
- Cerebrovascular and Carotid Artery Diseases
- Nuclear Structure and Function
- Chemotherapy-related skin toxicity
- Plant biochemistry and biosynthesis
- Lipid metabolism and biosynthesis
Institute of Cellular and Organismic Biology, Academia Sinica
2017-2025
National Taiwan University
2019-2025
National Defense Medical Center
2002-2025
National Taipei University of Nursing and Health Science
2021-2023
Taoyuan Chang Gung Memorial Hospital
2021-2023
Academia Sinica
2018-2022
Stanford University
2007-2011
Howard Hughes Medical Institute
2007-2011
Taichung Veterans General Hospital
1994-1999
Northwestern University
1989-1996
This study describes the development and use of a specific method for disassembling intermediate filament (IF) networks in living cells. It takes advantage disruptive effects mimetic peptides derived from amino acid sequence helix initiation 1A domain IF protein chains. The results demonstrate that at 1:1 molar ratios, these disassemble vimentin into small oligomeric complexes monomers within 30 min room temperature vitro. Upon microinjection cultured fibroblasts, same induce rapid...
As baby hamster kidney (BHK-21) cells enter mitosis, networks of intermediate filaments (IFs) are transformed into cytoplasmic aggregates protofilaments. Coincident with this morphological change, the phosphate content vimentin increases from 0.3 mol Pi per protein in interphase to 1.9 mitosis. A similar increase is observed desmin, 0.5 1.5 protein. Fractionation mitotic cell lysates by hydroxylapatite column chromatography reveals presence two IF kinase activities, designated as I and II....
Abstract The disassembly of vimentin-containing intermediate filament (IF) networks during mitosis in BHK-21 cells is accompanied by increased phosphorylation vimentin (Chou, Y.-H., Rosevear, E., and Goldman, R. D. (1989) Proc. Natl. Acad. Sci. U. S. A. 86, 1885-1889). We have recently identified p34cdc2 as the catalytic subunit one two endogenous kinases mitotic baby hamster kidney Bischoff, J. R., Beach, D., (1990) Cell 62, 1063-1071). To begin to characterize biochemical basis...
Significance This research reports that reciprocal interactions between Drosophila olfactory neurons and ensheathing glia mediate the formation of neuronal compartments, groups synapses are packed into discrete structures called “glomeruli” carry specific information. Ensheathing respond to a cue, FGF Thisbe, pattern boundaries nascent compartments. Neural in turn, require such glial barriers separate themselves from neighboring compartments thus ensure correct organization circuit. These...
Abstract Drosophila olfactory local interneurons (LNs) in the antennal lobe are highly diverse and variable. How when distinct types of LNs emerge, differentiate, integrate into circuit is unknown. Through systematic developmental analyses, we found that recruited to adult three groups. Group 1 residual larval LNs. 2 adult-specific emerge before cognate sensory projection neurons establish synaptic specificity, 3 after specificity established. selectively reintegrated through pruning...
The Drosophila Hedgehog receptor functions to regulate the essential downstream pathway component, Smoothened, and limit range of signaling by sequestering protein signal within imaginal disc epithelium. function requires both Patched Ihog activity, latter interchangeably encoded interference hedgehog (ihog) or brother ihog (boi). Here we show that activity are mutually required for endocytosis degradation, triggered binding, causing reduced levels Ihog/Boi proteins in a stripe cells at...
Several large or mid-scale collections of Drosophila enhancer traps have been recently created to allow for genetic swapping GAL4 coding sequences versatile transcription activators suppressors such as LexA, QF, split-GAL4 (GAL4-AD and GAL4-DBD), GAL80 QS. Yet a systematic analysis the feasibility reproducibility these tools is lacking. Here we focused on InSITE drivers that specifically label different subpopulations olfactory neurons, particularly local interneurons (LNs), genetically...
The crude actomyosin precipitate from sea urchin (Arbacia punctulata) egg extracts contains Ca2+-sensitive myosin light chain kinase activity. Activity can be further increased by exogenous calmodulin (CaM). Egg activity is purified total extract fractionating on three different chromatographic columns: DEAE ion exchange, gel filtration Sephacryl-300, and Affi-Gel-CaM affinity. depends totally Ca2+ CaM for Unphosphorylated has very little actin-activated ATPase. After phosphorylation of the...
During mitosis in BHK-21 baby hamster kidney cells the hyperphosphorylation of type III intermediate filament (IF) protein vimentin is accompanied by disruption IF network into punctate, protofilamentous structures. In this study, morphological and biochemical changes IFAP 300, a 300-kDa IF-crossbridging protein, are examined during mitosis. Double-label immunofluorescence shows that distribution 300 coincides with typical filamentous pattern displayed interphase cells, whereas mitotic it...
Local interneurons (LNs) in the Drosophila olfactory system exhibit neuronal diversity and variability, yet it is still unknown how these features impact information encoding capacity reliability a complex LN network. We employed two strategies to construct diverse excitatory-inhibitory neural network beginning with ring structure then introduced distinct types of inhibitory circuit variability simulated The continuity activity within node ensemble (oscillation pattern) was used as readout...
Variations in neuronal connectivity occur widely nervous systems from invertebrates to mammals. Yet, it is unclear how variability originates, what extent and at time scales exists, functional consequences might carry. To assess inter- intraindividual variability, would be ideal analyze the same identified neuron across different brain hemispheres individuals. Here, using genetic labeling electron microscopy connectomics, we show that an inhibitory olfactory local interneuron, TC-LN,...
PMA has both mitogenic and antiproliferative effects on human hepatoma Hep3B cells. In response to low concentration (10 nM), cells displayed an increasing proliferation potentiation. At high (1 µM) exhibited modest cytostatic effects. Determinations of protein kinase C (PKC) activity in PMA-treated revealed that alterations PKC are associated with proliferative capacity. The decrease mediated by a dose was accompanied cell growth inhibition. Increases were consistent stimulation. Immunoblot...
Intermediate filaments (IF) are composed of one or more members a large family proteins found in the nucleus and cytoplasm eukaryotic cells. In cytoplasm, they located between plasma membrane, forming complex arrays 10-nm diameter cytoskeletal (Goldman et al. 1986b). Five different types cytoplasmic IF have been described to date, which I–IV best characterized (Skalli Goldman 1991). Types I II keratins; type III includes such as desmin vimentin (Steinert Roop 1988); IV consists classic...
Highlights•A camera-mode treadmill system was built to track Drosophila walking trajectories•Four key features were identified describe strategies•Ball rotation is indispensable for full characterization of trajectories•Fed and starved control flies show no obvious differences in their random walkSummaryWalking trajectory frequently measured assess animal behavior. Air-supported spherical treadmills have been developed real-time monitoring trajectories. However, current systems mice mainly...
This study aimed to evaluate the effect of acupoint stimulation on pain, negative moods, and quality life for head neck cancer (HNC) patients who underwent concurrent chemoradiotherapy (CCRT). randomized controlled trial recruited participants from a medical center randomly assigned using permuted block randomization list with computer-generated random serial numbers into AcuCare group (n=46) receiving transcutaneous electrical (TAES) auricular acupressure (AA) or control without any...