A5070743974- Tissue Engineering and Regenerative Medicine
- Uterine Myomas and Treatments
- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Gynecological conditions and treatments
- Electrospun Nanofibers in Biomedical Applications
- COVID-19 Impact on Reproduction
- Reconstructive Surgery and Microvascular Techniques
- Pluripotent Stem Cells Research
- Organ and Tissue Transplantation Research
- Endometriosis Research and Treatment
- Advanced Proteomics Techniques and Applications
- Forensic Anthropology and Bioarchaeology Studies
- Genital Health and Disease
- Bone Tissue Engineering Materials
- Renal and related cancers
- Forensic and Genetic Research
- Wound Healing and Treatments
- Mesenchymal stem cell research
- Xenotransplantation and immune response
- Endometrial and Cervical Cancer Treatments
- Infectious Aortic and Vascular Conditions
- Ovarian function and disorders
- SARS-CoV-2 and COVID-19 Research
- Maternal and fetal healthcare
University of Gothenburg
2024-2025
Universitat de València
2020-2023
Instituto de Investigación Sanitaria La Fe
2019-2023
Valencian Infertility Institute
2021-2022
Decellularization techniques support the creation of biocompatible extracellular matrix hydrogels, providing tissue-specific environments for both in vitro cell culture and vivo tissue regeneration. We obtained endometrium derived from porcine decellularized uteri to create endometrial (EndoECM) hydrogels. After decellularization detergent removal, we investigated physicochemical features EndoECM, including gelation kinetics, ultrastructure, proteomic profile. The matrisome showed...
Organoids are three-dimensional (3D) multicellular tissue models that mimic their corresponding in vivo tissue. Successful efforts have derived organoids from primary tissues such as intestine, liver, and pancreas. For human uterine endometrium, the recent generation of 3D structures endometrial cells is inspiring new studies this important using precise preclinical models. To improve on these models, we decellularized pig endometrium containing tissue-specific extracellular matrix generated...
Research aimed at preserving female fertility is increasingly using bioengineering techniques to develop new platforms capable of supporting ovarian cell function in vitro and vivo. Natural hydrogels (alginate, collagen, fibrin) have been the most exploited approaches; however they are biologically inert and/or biochemically simple. Thus, establishing a suitable biomimetic hydrogel from decellularized cortex (OC) extracellular matrix (OvaECM) could provide complex native biomaterial for...
Abstract Background Uterus tissue engineering aims to repair a dysfunctional uterus that causes infertility, e.g., after significant scarring from benign or malign resection procedures. Decellularized uterine provided regenerative support in several animal models as biocompatible natural extracellular matrix (ECM) derived scaffold damage. However, variations decellularization protocols and species used limit conclusive evidence translational progress. Hence, species-independent protocol...
Abstract COVID-19 exerts systemic effects that can compromise various organs and systems. Although retrospective in silico studies prospective preliminary analysis have assessed the possibility of direct infection endometrium, there is a lack in-depth on impact disease key endometrial genes functions across menstrual cycle window implantation. Gene expression data been obtained from (i) healthy secretory endometrium collected 42 women without pathologies (ii) nasopharyngeal swabs 231 with 30...
Abstract Introduction Uterus bioengineering offers a potential treatment option for women with uterine factor infertility and mitigating the risk of rupture associated defective tissue. Decellularized tissue scaffolds proved promising in further vivo experiments rodent domestic species animal models. Variations extracellular matrix composition among different adaptations decellularization protocols make it difficult to compare results between studies. Therefore, we assessed if our earlier...
Abstract Purpose Fertility preservation for pre-pubertal girls undergoing gonadotoxic cancer treatments and women with systemic cancers at high risk ovarian metastasis remains limited. Current options, such as cortex transplantation, reintroducing malignant cells. This study presents a novel approach focusing on ex vivo folliculogenesis mature oocyte retrieval cryopreservation, mitigating this risk. Methods experimental optimized an perfusion system in sheep, refining gonadotropin...
Objective Determining genetic and paracrine mechanisms behind endometrial regeneration in Asherman's syndrome atrophy (AS/EA) patients after autologous CD133 + bone marrow‐derived stem cell (CD133 BMDSC) transplantation. Design Retrospective study using human biopsies mouse models. Setting Fundación‐IVI, IIS‐La Fe, Valencia, Spain. Samples Endometrial collected before BMDSC therapy, from eight women with AS/EA (NCT02144987) the uterus of five mice only left horns receiving therapy. Methods...
Abstract STUDY QUESTION Can human umbilical cord platelet-rich plasma (hUC-PRP) efficiently treat endometrial damage and restore fertility in a preclinical murine model? SUMMARY ANSWER Local application of hUC-PRP promotes tissue regeneration restoration model Asherman syndrome atrophy (AS/EA). WHAT IS KNOWN ALREADY AS/EA are well-described pathologies that cause infertility; however, there currently no gold-standard treatments available. Recent reports have described the successful use...
Uterine leiomyoma (UL) is a benign tumor arising from myometrium (MM) with high prevalence and unclear pathology. Histone modifications are altered in tumors, particularly via histone acetylation which correlated gene activation. To identify if the of H3K27 involved UL pathogenesis its reversion may be therapeutic option, we performed prospective study integrating RNA-seq (n = 48) CHIP-seq for H3K27ac 19) vs MM tissue, together qRT-PCR SAHA-treated cells 10). showed lower levels versus...
Transplantation of decellularized uterus tissue showed promise in supporting regeneration following uterine injury animal models, suggesting an alternative to complete transplantation for factor infertility treatment. However, most studies utilized small grafts, limiting their clinical relevance. Hence, we used larger grafts (20 × 10 mm), equivalent nearly one horn rats, better evaluate the bioengineering challenges associated with structural support, revascularization, and regeneration. We...
Abstract Study question Can we regenerate a damaged uterus using decellularized (further repopulated with stem cells or not) uterine scaffolds in sheep model? Summary answer Bioengineered grafts can promote the regeneration of uterus, but this process is highly dependent on basal immune status recipient. What known already Uterus bioengineering aims to develop repair defective tissue that may cause infertility. The remaining extracellular matrix after decellularization has frequently been...
Abstract Study question Does histone mark H3K27ac regulate the expression of genes involved in uterine leiomyoma (UL) pathogenesis, and can deacetylation inhibition be a new therapeutic approach? Summary answer regulates implicated UL pathogenesis through angiogenesis extracellular matrix (ECM), reversion could offer approach to treat UL. What is known already benign tumor arising from myometrium (MM). Women present symptoms such as pelvic pain infertility. There no fully effective treatment...