A5003414220- Reproductive Biology and Fertility
- Ovarian function and disorders
- Reproductive System and Pregnancy
- Sperm and Testicular Function
- Renal and related cancers
- Endometriosis Research and Treatment
- Pluripotent Stem Cells Research
- Ovarian cancer diagnosis and treatment
- Tissue Engineering and Regenerative Medicine
- Uterine Myomas and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Effects and risks of endocrine disrupting chemicals
- Prenatal Screening and Diagnostics
- Genetics, Aging, and Longevity in Model Organisms
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Pregnancy and preeclampsia studies
- Advanced Proteomics Techniques and Applications
- Molecular Biology Techniques and Applications
- Agriculture, Soil, Plant Science
- Cancer Cells and Metastasis
Instituto de Investigación Sanitaria La Fe
2018-2025
Valencian Infertility Institute
2017-2024
Carrier (United States)
2021
Cohort (United Kingdom)
2021
Universitat de València
2017-2019
Istituto di Istruzione Superiore I.T.C. Di Vittorio
2019
Abstract STUDY QUESTION Does LH protect mouse oocytes and female fertility from alkylating chemotherapy? SUMMARY ANSWER treatment before during chemotherapy prevents detrimental effects on follicles reproductive lifespan. WHAT IS KNOWN ALREADY Chemotherapies can damage the ovary, resulting in premature ovarian failure reduced cancer survivors. was recently suggested to prepubertal chemotoxic of cisplatin treatment. DESIGN, SIZE, DURATION This experimental study investigated primordial...
Chemotherapy-induced ovarian damage represents a major challenge for women of reproductive age undergoing cancer treatments. In mice, nicotinamide mononucleotide (NMN) enhances the developmental competence aged oocytes by preventing oxidative stress, inflammation, and apoptosis, however its potential to restore oocyte quality following chemotherapy-induced remains unexplored. To investigate effects NMN treatment against deleterious chemotherapy aging in mice human oocytes. We used mouse...
Premature ovarian insufficiency (POI) means menopause before 40 years of age affecting about 1 % women. Approaches based on cell therapy and the paracrine effects stem cells or bioproducts such as platelet-rich plasma have been proposed, but concerns remain undesired systemic effects, well need to optimize delivery methods through bioengineering methods. This study explores efficacy decellularized bovine cortex extracellular matrix (OvaECM) hydrogels alone a growth factor (GF) carrier...
To date, several groups have generated homologous models of endometriosis through the implantation endometrial tissue fluorescently labeled by green fluorescent protein (GFP) or from luciferase-expressing transgenic mice into recipient animals, enabling noninvasive monitoring lesion signal. These present an advantage over endpoint models, but some limitations persist; use is laborious and expensive, GFP presents poor penetration due to relatively short emission wavelength. For this reason, a...
Female fertility is negatively correlated with age, noticeable declines in oocyte quantity and quality until menopause. To understand this physiological process evaluate human approaches for treating age-related infertility, preclinical studies appropriate animal models are needed. Thus, we aimed to characterize an immunodeficient aging mouse model displaying ovarian characteristics of different stages during women's reproductive life. NOD/SCID mice ages (8-, 28-, 36–40-week-old) were...
Patients with poor ovarian response (POR) and premature insufficiency (POI) are challenging to treat, oocyte donation remaining as the only feasible option achieve pregnancy in some cases. The Autologous stem cell transplantation (ASCOT) technique allows follicle development, enabling pregnancies births of healthy babies these patients. Previous results suggest that growth factors cytokines secreted by cells partially responsible for their regenerative properties. Indeed, ASCOT beneficial...
To analyze the role of PAI-1 (plasminogen activator inhibitor 1) in endometriotic lesion growth, we studied effect inhibition by PAI-039 using a homologous mouse model endometriosis that allows noninvasive monitoring. Endometrial tissue from donor mice was collected, labeled with mCherry adenovirus, and implanted into subcutaneous pocket on ventral abdomen recipient mice. Seven days after transplantation, were randomly allocated two groups treated once daily for 2 weeks either vehicle...
Abstract Study question Can the new IVFA therapy effectively promote ovarian follicle development and increase oocyte number in a premature insufficiency (POI) animal model. Summary answer The technique reveals favourable reproductive outcomes model, demonstrating increased retrieval without compromising quality, making promising advancement fertility restoration. What is known already POI characterized by loss of primordial follicular pool before age 40. Ovarian fragmentation for vitro...
Abstract Study question What mechanisms are involved in the ovarian alterations of Polycystic Ovary Syndrome (PCOS) and Morphology? Summary answer PCOS patients associated a distinctive impairment oxidative phosphorylation dysregulated complement system their follicular compartments not found Ovarian Morphology (PCOM) women. is known already major cause female infertility characterized by aberrant development hormonal imbalances leading to reproductive symptoms such as anovulation, poor...
Abstract Study question Does the 4-step ASCOT technique induce any modification in plasma proteomic profile of women with premature ovarian insufficiency (POI)? Summary answer The elicits substantial changes proteome POI patients, leading to a more similar composition that observed normoresponder women. What is known already Nowadays, treatment still challenging and different approaches have been proposed improve their reproductive potential, including stem cells (SC) platelet-rich (PRP)....
Abstract Study question Does NMN supplementation improve nuclear and cytoplasmic competence of AMA oocytes during GV-rescue? Summary answer enhances GV-rescue efficiency in by improving slightly ameliorating competence. What is known already Oocyte drastically declines from the age 35 onwards. With age, organism NAD+ levels also drop, depletion at oocyte level associated with lower embryo quality. Over past years, precursor nicotinamide mononucleotide (NMN) has been showed to recover quality...
(Abstracted from Fertil Steril 2019;111(1):86–96) Initial phases of embryonic development have a high requirement for ATP, and evidence shows that oocytes with ATP levels enhance implantation the human embryo. Decreased bioenergetic capacity in has been found to be primary contributor declining embryo quality advancing maternal age or women premature ovarian aging.
Abstract Study question Does autologous stem cell ovarian transplant (ASCOT) induce any long-term modifications in the plasma proteomic profile of women with premature insufficiency (POI)? Summary answer Stem mobilization and infusion into ovary elicit changes peripheral blood composition POI, reverting age-related changes. What is known already Patients POI are challenging to treat, oocyte donation remaining as only feasible option achieve pregnancy. Previous attempts overcome fertility...
Abstract Study question Does the D19S884 allele 8 (A8) equally affect pathogenesis and ovarian gene expression of PCOS PCOM patients? Summary answer A8-allele produces metabolic, endocrine, alterations regardless diagnose. The mechanisms involved in are different from those phenotype PCOS. What is known already specific FBN3 may be related to polycystic syndrome (PCOS) clinical manifestations. A8 participates alternative splicing Asprosin-3, glucose modulation, Fibrillin-3. Fibrilin-3 an...