A5032363129- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Insect Resistance and Genetics
- Insect and Arachnid Ecology and Behavior
- Cancer-related gene regulation
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Plant Virus Research Studies
- Cancer Immunotherapy and Biomarkers
- Advanced biosensing and bioanalysis techniques
- Microtubule and mitosis dynamics
- Nuclear Structure and Function
- RNA modifications and cancer
- Plant Gene Expression Analysis
- Heat shock proteins research
- Genetics and Neurodevelopmental Disorders
- Bacterial Genetics and Biotechnology
Centre National de la Recherche Scientifique
2011-2024
Sorbonne Université
2009-2024
Université Paris Sciences et Lettres
2016-2024
Université Paris-Saclay
2023-2024
La Ligue Contre le Cancer
2014-2024
Institut Curie
2014-2024
Dynamique du noyau
2012-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
1997-2024
Direction de la Recherche Fondamentale
2024
CEA Paris-Saclay
2024
After priming, naïve CD8+ T lymphocytes establish specific heritable transcription programs that define progression to long-lasting memory cells or short-lived effector cells. Although lineage specification is critical for protection, it remains unclear how chromatin dynamics contributes the control of gene expression programs. We explored role silencing by histone methyltransferase Suv39h1. In murine activated after Listeria monocytogenes infection, Suv39h1-dependent trimethylation H3...
Sensing DNA damage is crucial for the maintenance of genomic integrity and cell cycle progression. The participation chromatin in these events becoming increasing interest. We show that presence single-strand breaks gaps, formed either directly or during processing, can trigger propagation nucleosomal arrays. This nucleosome assembly pathway involves histone chaperone factor 1 (CAF-1). largest subunit (p150) this interacts with proliferating nuclear antigen (PCNA), critical regions...
During mammalian development, chromatin dynamics and epigenetic marking are important for genome reprogramming. Recent data suggest an role the assembly machinery in this process. To analyze of factor 1 (CAF-1) during pre-implantation we generated a mouse line carrying targeted mutation gene encoding its large subunit, p150CAF-1. Loss p150CAF-1 homozygous mutants leads to developmental arrest at 16-cell stage. Absence these embryos results severe alterations nuclear organization constitutive...
DNA replication is a challenge for the faithful transmission of parental information to daughter cells, as both and chromatin organization must be duplicated. Replication stress further complicates safeguard epigenome integrity. Here, we investigate histone variants H3.3 H3.1 during replication. We follow their distribution relative timing, first in genome and, second, 3D using super-resolution microscopy. find that mark early- late-replicating chromatin, respectively. In nucleus, forms...
Abstract Tumor-infiltrating CD8 + T cells progressively lose functionality and fail to reject tumors. The underlying mechanism re-programing induced by checkpoint blockers are incompletely understood. We show here that genetic ablation or pharmacological inhibition of histone lysine methyltransferase Suv39h1 delays tumor growth potentiates rejection anti-PD-1. In the absence Suv39h1, anti-PD-1 induces alternative activation pathways allowing survival differentiation IFNγ Granzyme B producing...
The human HIRA gene has been named after Hir1p and Hir2p, two corepressors which together appear to act on chromatin structure control transcription in Saccharomyces cerevisiae.HIRA homologs are expressed a regulated fashion during mouse chicken embryogenesis, the is major candidate for DiGeorge syndrome related developmental disorders caused by reduction single dose of fragment chromosome 22q.Western blot analysis double-immunofluorescence experiments using specific antiserum revealed...
Single strand nicks and gaps in DNA have been reported to increase the efficiency of nucleosome loading mediated by chromatin assembly factor 1 (CAF-1). However, on mismatch-containing substrates, these discontinuities are utilized mismatch repair (MMR) system as sites for exonuclease 1, at which degradation error-containing commences. Because packaging into might inhibit MMR, we were interested learn whether is differentially regulated heteroduplex homoduplex substrates. We now show that...
Fluorescence videomicroscopy and scanning force microscopy were used to follow, in real time, chromatin assembly on individual DNA molecules immersed cell-free systems competent for physiological assembly. Within a few seconds, are already compacted into form exhibiting strong similarities native fibers. In these extracts, the compaction rate is more than 100 times faster expected from standard biochemical assays. Our data provide definite information forces involved (a piconewtons) reaction...
HP1 enrichment at pericentric heterochromatin is essential for proper chromosome segregation. While H3K9me3 thought to be a major contributor domains, in mouse cells, the SUMO-protease SENP7 required addition H3K9me3. How this achieved remains elusive. Here, we find that loss of leads an increased time spent mitosis. Furthermore, reveal short module comprising two consecutive interaction motifs on determinant and acts by restricting mobility domains. We propose mechanism maintenance which...
Understanding the mechanisms that lead to replication fork blocks (RFB) and means bypass them is important given threat they represent for genome stability if inappropriately handled. Here, study this issue in mammals, we use integrated arrays of LacO and/or TetO as a tractable system follow time process an individual cell at single locus. Importantly, show induction binding by LacI TetR proteins, not presence repeats, key form RFB. We find proteins during causes prolonged persistence foci...