A5100685901- CAR-T cell therapy research
- Virus-based gene therapy research
- Cancer, Hypoxia, and Metabolism
- Cholesterol and Lipid Metabolism
- Drug Transport and Resistance Mechanisms
- Biochemical and Molecular Research
- CRISPR and Genetic Engineering
- Breast Cancer Treatment Studies
- Diagnosis and Treatment of Venous Diseases
- Liver Disease Diagnosis and Treatment
- Global Cancer Incidence and Screening
- Palliative Care and End-of-Life Issues
- Epigenetics and DNA Methylation
- European and International Law Studies
- Law and Political Science
- Immunotherapy and Immune Responses
- Breast Lesions and Carcinomas
- Atherosclerosis and Cardiovascular Diseases
- Critical Race Theory in Education
- Sphingolipid Metabolism and Signaling
- Breast Implant and Reconstruction
- Criminal Law and Policy
- Pressure Ulcer Prevention and Management
- HIV Research and Treatment
- Viral Infectious Diseases and Gene Expression in Insects
University of California, Los Angeles
2018-2025
Kessler Foundation
2025
University of Colorado Denver
2025
University of Colorado Anschutz Medical Campus
2025
Side by Side
2024
Rockefeller University
2015-2022
Temple University
2022
Ronald Reagan UCLA Medical Center
2020
UCLA Jonsson Comprehensive Cancer Center
2018-2019
Cook County Health and Hospitals System
2019
Abstract Individual cells within a tumour can exhibit distinct genetic and molecular features. The impact of such diversification on metastatic potential is unknown. Here we identify clonal human breast cancer subpopulations that display different levels morphological diversity. Highly variable are more proficient at colonization chemotherapeutic survival. Through single-cell RNA-sequencing, inter-cell transcript expression variability identified as defining feature the highly leads to...
Intestinal absorption is an important contributor to systemic cholesterol homeostasis. Niemann-Pick C1 Like 1 (NPC1L1) assists in the initial step of dietary uptake, but how moves downstream NPC1L1 unknown. We show that Aster-B and Aster-C are critical for nonvesicular movement enterocytes. Loss diminishes accessible plasma membrane (PM) abolishes Aster recruitment intestinal brush border. Enterocytes lacking Asters accumulate PM endoplasmic reticulum depletion. Aster-deficient mice have...
Colorectal cancer (CRC) is a major cause of human death. Mortality primarily due to metastatic organ colonization, with the liver being main affected. We modeled CRC (mCRC) colonization using patient-derived primary and tumor xenografts (PDX). Such PDX modeling predicted patient survival outcomes. In vivo selection multiple PDXs for enhanced capacity upregulated gluconeogenic enzyme PCK1, which growth by driving pyrimidine nucleotide biosynthesis under hypoxia. Consistently, highly tumors...
Colorectal cancer metastasis to the liver is a major cause of cancer-related death; however, genes and pathways that govern this metastatic colonization event remain poorly characterized. Here, using large-scale in vivo RNAi screen, we identified red blood cell pyruvate kinase (PKLR) as driver colonization. PKLR expression was increased metastases well primary colorectal tumors patients with disease. Evaluation murine model revealed promotes survival tumor core during conditions high density...
Non-alcoholic fatty liver disease (NAFLD) is expected to become a leading aetiology of hepatocellular carcinoma (HCC)-related mortality in the United States. HCC treatments with curative intent (OLT, orthotopic transplantation; resection; RFA, radiofrequency ablation) can improve survival carefully selected patients.To compare after receipt treatment for NAFLD and non-NAFLD-HCC aetiologies (HCV, chronic hepatitis C; HBV, B; ALD, alcoholic disease) by was performed.A cohort 17 664 patients...
Matrix metalloproteinase-2 (MMP-2) plays a major role in dysfunctional ventricular remodeling following myocardial injury induced by ischemia/reperfusion and heart failure. To directly assess the of MMP-2 absence superimposed injury, we generated cardiac-specific, constitutively active transgenic mice.Morphologic functional studies were carried out using both intact demembranated (skinned) right trabeculae dissected from hearts 8-month-old mice wild-type controls (WT).Electron micrographs...
The human MLL genes (MLL1 to MLL4) and their Drosophila orthologs, trithorax (trx) related (trr), encode proteins capable of methylating histone H3 on lysine 4. MLL1 MLL2 are most similar trx, while MLL3 MLL4 more closely trr. Several mutated in cancers, but how these regulate cell proliferation is not known. Here we show that trr mutant cells have a growth advantage over wild-type neighbors display changes the levels multiple division, including Notch, Capicua, cyclin B. clones markedly...
Abstract Introduction Chronic venous stasis ulcers are complex and challenging to manage, often resulting in prolonged healing poor outcomes with standard wound care. This case report presents a 66-year-old female medical history of pyoderma gangrenosum, hypothyroidism, mitral valve prolapse, ulcerative colitis, chronic insufficiency, who developed symmetric on both lower extremities. The patient had long-standing ulcer the left leg (15 years) newer right (4 years). After multiple...
Human single nucleotide variants in peroxisome proliferator-activated receptor-ɑ (PPARɑ) have been associated with beneficial metabolic phenotypes, yet their specific effects on gene expression are not well defined. Here, we developed a mouse model of human PPARɑ variant encoding substitution valine for alanine at position 227 (V227A) to explore the role this systemic metabolism. Substitution mice reduced plasma triglycerides, without altering body mass or liver lipid accumulation,...
In breast conserving surgery (BCS), the usefulness to perform systematic cavity shaving is actively debated. Some investigators argued that could avoid surgical re-excision and make diagnosis of unexpected multifocality. Others depends on volumes resection. this study one hundred patients undergoing BCS with were included. Margins less than 3 mm considered be insufficient. We tested clinico-pathological characteristics in order identify predictive model margin utility we sought determine if...
Significance The liver has a complex cellular architecture that is essential for its function. In this work, we show BRAP, ubiquitin ligase of poorly understood function, required maintenance proper morphology. BRAP deletion in the causes disruption normal and inflammation due to altered expression genes involved cell growth extracellular interactions. This work sheds light on mechanisms maintain structure implications disease.
To improve persistence of adoptively transferred T-cell receptor (TCR)-engineered T cells and durable clinical responses, we designed a trial to transplant genetically-modified hematopoietic stem (HSCs) together with adoptive cell transfer both engineered express an NY-ESO-1 TCR. Here, report the preclinical studies performed enable investigational new drug (IND) application.HSCs transduced lentiviral vector expressing TCR PET reporter/suicide gene HSV1-sr39TK retroviral were coadministered...
Intestinal cholesterol absorption is an important contributor to systemic homeostasis. Niemann-Pick C1 Like 1 (NPC1L1), the target of drug ezetimibe (EZ), assists in initial step dietary uptake. However, how moves downstream NPC1L1 unknown. Here we show that Aster-B and Aster-C are critical for non-vesicular movement enterocytes, bridging at plasma membrane (PM) ACAT2 endoplasmic reticulum (ER). Loss diminishes accessible PM enterocytes abolishes Aster recruitment intestinal brush border....
INTRODUCTION: The timing of when to perform endoscopy in patients with a positive cocaine urine drug screen (UDS+C) varies across institutions and is based on expert opinions personal experience. There are no clear published guidelines or reported studies the English literature examining safety active users cocaine. This study aimed describe incidence adverse events esophagogastroduodenoscopy (EGD) use single inner-city tertiary hospital Chicago. METHODS: We examined consecutive records who...
Abstract The intrinsic pathways that control membrane organization in immune cells and the impact of such on cellular function are not well defined. Here we report non-vesicular cholesterol transporter Aster-A links plasma (PM) availability T to signaling systemic metabolism. is recruited PM during T-cell receptor (TCR) activation, where it facilitates removal newly generated “accessible” cholesterol. Loss leads excess accumulation, resulting enhanced TCR nano-clustering signaling, Th17...
In 2030 Ethnic Studies will be a required course for all high schoolers in California's public schools. This paper examines the opportunities and challenges of this milestone how future can impact U.S. education system as whole. Through highlighting history presenting perspectives eight early career teachers counselors, study showcases importance providing spaces students Color to see themselves school curricula, build community, envision substantive change through their teaching.
Abstract Hypercholesterolemia has long been implicated in endothelial cell (EC) dysfunction, but the mechanisms by which excess cholesterol causes vascular pathology are incompletely understood. Here we used a cholesterol-mimetic probe to map cholesterol-protein interactions primary human ECs and discovered that binds stabilizes adhesion molecule VCAM-1. We show accessible plasma membrane (PM) is acutely responsive inflammatory stimuli nonvesicular transporter Aster-A regulates VCAM-1...
Abstract Colorectal cancer (CRC) is a major cause of human death. Mortality primarily due to metastatic organ colonization, with liver being the primary affected. We modeled CRC (mCRC) colonization using patient-derived and tumor xenografts (PDX). Such PDX modeling predicted patient survival outcomes. In vivo selection multiple PDXs for enhanced capacity upregulated gluconeogenic enzyme PCK1, which hypoxic by driving anabolic pyrimidine nucleotide biosynthesis. Consistently, highly tumors...