A5076081554- Genetic Associations and Epidemiology
- Bone health and osteoporosis research
- Bone Metabolism and Diseases
- Genetic Mapping and Diversity in Plants and Animals
- Ubiquitin and proteasome pathways
- Genetic and phenotypic traits in livestock
- Genomic variations and chromosomal abnormalities
- Genomics and Rare Diseases
- Folate and B Vitamins Research
- Immune Response and Inflammation
- Bacterial Infections and Vaccines
- Growth Hormone and Insulin-like Growth Factors
- NF-κB Signaling Pathways
- Bacterial Identification and Susceptibility Testing
- Gut microbiota and health
- Mycobacterium research and diagnosis
- Clostridium difficile and Clostridium perfringens research
- Nutrition, Genetics, and Disease
- Bone health and treatments
- Orthopedic Infections and Treatments
- Ion channel regulation and function
- Adipose Tissue and Metabolism
- Escherichia coli research studies
- interferon and immune responses
- Liver Disease Diagnosis and Treatment
University of Missouri–Kansas City
2011-2023
University of Maryland, Baltimore
2005-2016
National Institute of Allergy and Infectious Diseases
2013-2016
Albert Einstein College of Medicine
2016
Duke Medical Center
2016
University of Florida
2016
Vanderbilt University
2016
Tulane University
2011-2016
National Institutes of Health
2016
SimulConsult
2013
The human gut microbiome can modulate metabolic health and affect insulin resistance, it may play an important role in the etiology of gestational diabetes mellitus (GDM). Here, we compared microbial composition 43 GDM patients 81 healthy pregnant women via whole-metagenome shotgun sequencing their fecal samples, collected at 21-29 weeks, to explore associations between taxonomic units functional genes. A metagenome-wide association study identified 154 837 genes, which clustered into 129...
Obesity is a major public health problem with strong genetic determination; however, the factors underlying obesity are largely unknown. In this study, we performed genome-wide association scan for by examining approximately 500 000 single-nucleotide polymorphisms (SNPs) in sample of 1000 unrelated US Caucasians. We identified novel gene, CTNNBL1, which has multiple SNPs associated body mass index (BMI) and fat mass. The most significant SNP, rs6013029, achieved experiment-wise P-values 2.69...
The human zona pellucida is composed of four glycoproteins (ZP1, ZP2, ZP3, and ZP4) has an important role in reproduction. Here we describe a form infertility with autosomal recessive mode inheritance, characterized by abnormal eggs that lack pellucida. We identified homozygous frameshift mutation ZP1 six family members. In vitro studies showed defective proteins normal ZP3 colocalized throughout the cells were not expressed at cell surface, suggesting aberrant results sequestration...
Altered immune function during ageing results in increased production of nitric oxide (NO) and other inflammatory mediators. Recently, we have reported that NO was inhibited by naturally-occurring proteasome inhibitors (quercetin, δ-tocotrienol, riboflavin) lipopolysaccharide (LPS)-stimulated RAW264.7 cells, thioglycolate-elicited peritoneal macrophages from C57BL/6 mice. In a continuous effort to find more potent, non-toxic, commercially available, suppress inflammation, the present study...
Abstract Motivation: Several new de novo assembly tools have been developed recently to assemble short sequencing reads generated by next-generation platforms. However, the performance of these under various conditions has not fully investigated, and sufficient information is currently available for informed decisions be made regarding tool that would most likely produce best a specific set conditions. Results: We studied compared commonly used specifically designed data, including SSAKE,...
Abstract Although the gut microbiota has been reported to influence osteoporosis risk, individual species involved, and underlying mechanisms, remain largely unknown. We performed integrative analyses in a Chinese cohort of peri-/post-menopausal women with metagenomics/targeted metabolomics/whole-genome sequencing identify novel microbiome-related biomarkers for bone health. Bacteroides vulgatus was found be negatively associated mineral density (BMD), which validated US white people. Serum...
The power of genetic association analyses is often compromised by missing genotypic data which contributes to lack significant findings, e.g., in silico replication studies. One solution impute untyped SNPs from typed flanking markers, based on known linkage disequilibrium (LD) relationships. Several imputation methods are available and their usefulness studies has been demonstrated, but factors affecting relative performance accuracy have not systematically investigated. Therefore, we...
Osteoporosis is a major public health problem. It mainly characterized by low bone mineral density (BMD) and/or low-trauma osteoporotic fractures (OF), both of which have strong genetic determination. The specific genes influencing these phenotypic traits, however, are largely unknown. Using the Affymetrix 500K array set, we performed case-control genome-wide association study (GWAS) in 700 elderly Chinese Han subjects (350 with hip OF and 350 healthy matched controls). A follow-up...
Wrist fracture is not only one of the most common osteoporotic fractures but also a predictor future at other sites. bone mineral density (BMD) an important determinant wrist risk, with high heritability. Specific genes underlying BMD variation are largely unknown. Most published genome-wide association studies (GWASs) have focused on few top-ranking single-nucleotide polymorphisms (SNPs)/genes and considered each identified SNPs/genes independently. To identify biologic pathways to...
MicroRNAs (miRNAs) regulate posttranscriptional gene expression usually by binding to 3'-untranslated regions (3'-UTRs) of target message RNAs (mRNAs). Hence genetic polymorphisms on 3'-UTRs mRNAs may alter affinity between miRNAs 3'-UTRs, thereby altering translational regulation and/or degradation mRNAs, leading differential protein genes. Based a database that catalogues predicted in miRNA sites (poly-miRTSs), we selected 568 within and performed association analyses these poly-miRTSs...
Intermittent application of parathyroid hormone (PTH) has well established anabolic effects on bone mass in rodents and humans. Although transcriptional mechanisms responsible for these are not fully understood, it is recognized that factor cAMP response element binding protein (CREB) mediates PTH signaling osteoblasts, there a communication between the PTH-CREB pathway BMP2 pathway, which important osteoblast differentiation formations. These findings, conjunction with putative elements...
Inflammation has been implicated in a variety of diseases associated with ageing, including cancer, cardiovascular, and neurologic diseases. We have recently established that the proteasome is pivotal regulator inflammation, which modulates induction inflammatory mediators such as TNF-α, IL-1, IL-6, nitric oxide (NO) response to stimuli. The present study was undertaken identify non-toxic inhibitors expectation these compounds could potentially suppress production ageing humans, thereby...
Whole genome sequencing studies are essential to obtain a comprehensive understanding of the vast pattern human genomic variations. Here we report results high-coverage whole study for 44 unrelated healthy Caucasian adults, each sequenced over 50-fold coverage (averaging 65.8×). We identified approximately 11 million single nucleotide polymorphisms (SNPs), 2.8 short insertions and deletions, 500,000 block substitutions. showed that, although previous studies, including 1000 Genomes Project...