A5000165068- Plant Molecular Biology Research
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Circadian rhythm and melatonin
- Cardiac electrophysiology and arrhythmias
- Animal Genetics and Reproduction
- ECG Monitoring and Analysis
- Heart Rate Variability and Autonomic Control
- Plant nutrient uptake and metabolism
- Zebrafish Biomedical Research Applications
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Genetics, Aging, and Longevity in Model Organisms
- Kruppel-like factors research
- Plant Genetic and Mutation Studies
- Genomic variations and chromosomal abnormalities
- Plant Gene Expression Analysis
- Genetic Associations and Epidemiology
- Genetic Syndromes and Imprinting
- Photoreceptor and optogenetics research
- Olfactory and Sensory Function Studies
- Cancer Genomics and Diagnostics
- Chronic Lymphocytic Leukemia Research
- Zoonotic diseases and public health
- Memory and Neural Mechanisms
University of Cambridge
2013-2022
Cambridge University Hospitals NHS Foundation Trust
2022
University College London
2020-2021
New York Proton Center
2011
Ithaca College
2011
RELX Group (United Kingdom)
2011
Communities In Schools of Orange County
2011
University of Birmingham
2011
Advisory Board Company (United States)
2011
Parker Hannifin (Germany)
2011
Hundreds of human genes are associated with neurological diseases, but translation into tractable biological mechanisms is lagging. Larval zebrafish an attractive model to investigate genetic contributions diseases. However, current CRISPR-Cas9 methods difficult apply large screens studying behavioural phenotypes. To facilitate rapid screening, we developed a simple sequencing-free tool validate gRNAs and highly effective method capable converting >90% injected embryos directly F0...
Synchronization of circadian clocks to the day-night cycle ensures correct timing biological events. This entrainment process is essential ensure that phase oscillator synchronized with daily events within environment [1], permit accurate anticipation environmental changes [2, 3]. Entrainment in plants requires oscillator, through unidentified pathways, which alter gene expression response light, temperature, and sugars [4-6]. To determine how respond metabolic rhythms, we investigated...
Abstract Arabidopsis plants store part of the carbon fixed by photosynthesis as starch to sustain growth at night. Two competing hypotheses have been proposed explain this diel turnover based on either measurement abundance with respect circadian time, or sensing sugars feedback oscillator dynamically adjust timing turnover. We report a phase model that permitted derivation ideal responses regulation breakdown maintain sucrose homeostasis. Testing predictions using sugar-unresponsive mutant...
Circadian clocks drive rhythms with a period near 24 h, but the molecular basis of regulation circadian clockis poorly understood. We previously demonstrated that metabolites affect free-running oscillator Arabidopsis (Arabidopsis thaliana), endogenous sugars acting as an accelerator and exogenous nicotinamide brake. Changes in are thought to adjust timing biological activities through process entrainment, which becomes synchronized rhythmic signals such light dark cycles well changes...
The circadian oscillator, an internal time-keeping device found in most organisms, enables timely regulation of daily biological activities by maintaining synchrony with the external environment. mechanistic basis underlying adjustment rhythms to changing conditions, however, has yet be clearly elucidated. We explored mechanism action nicotinamide Arabidopsis thaliana, a metabolite that lengthens period rhythms, understand period. To identify key mechanisms involved response nicotinamide, we...
L-Carnitine (16 mmoles per kg, injected intraperitoneally) is reported to protect mice against subsequent injection of ammonium acetate given at the unprotected LD100. The present studies in rats show a variable protective effect L-carnitine kg) administered 1 hr prior an LD100 dose acetate. Survival ranged from 100% 35%. In two experiments, protection was highly significant; third experiment, did not death but significantly prolong time death. Although cause this variability known, data...
<ns4:p>Circadian clocks have evolved to synchronise an organism’s physiology with the environmental rhythms driven by Earth’s rotation on its axis. Over past two decades, many of genetic components <ns4:italic>Arabidopsis thaliana</ns4:italic> circadian oscillator been identified. The interactions between these formulized into mathematical models that describe transcriptional translational feedback loops oscillator. More recently, focus has turned regulation and functions clock. These...
ABSTRACT Hundreds of human genes are associated with neurological diseases, but translation into tractable biological mechanisms is lagging. Larval zebrafish an attractive model to investigate genetic contributions diseases. However, current CRISPR-Cas9 methods difficult apply large screens studying behavioural phenotypes. To facilitate rapid screening, we developed a simple sequencing-free tool validate gRNAs and highly effective method capable converting >90% injected embryos directly...
Abstract The role of the circadian clock is becoming apparent in many aspects human health and disease. Large scale GWAS studies have delivered high numbers genetic markers for chronotype, which can be used to find links Mendelian disorders. We variants 1,000 genomes study estimate linkage disequilibrium these chronotype markers. analysed genes with enrichment disease-causing genes, looked common HPO terms wider disease associated genes. identified that two cardiovascular disorders,...