A5047561740- Circadian rhythm and melatonin
- Photoreceptor and optogenetics research
- Concrete Corrosion and Durability
- Genetics, Aging, and Longevity in Model Organisms
- Spaceflight effects on biology
- Light effects on plants
- Smart Materials for Construction
- Corrosion Behavior and Inhibition
- COVID-19 Clinical Research Studies
- Neurobiology and Insect Physiology Research
- Marine Ecology and Invasive Species
- Epigenetics and DNA Methylation
- Infrastructure Maintenance and Monitoring
- Structural Behavior of Reinforced Concrete
- Subterranean biodiversity and taxonomy
- Advanced Energy Technologies and Civil Engineering Innovations
- Long-Term Effects of COVID-19
- Animal Genetics and Reproduction
- Intensive Care Unit Cognitive Disorders
- Herpesvirus Infections and Treatments
- Geotechnical Engineering and Underground Structures
- Structural Integrity and Reliability Analysis
- SARS-CoV-2 and COVID-19 Research
- Turtle Biology and Conservation
- Gene Regulatory Network Analysis
University College London
2014-2024
James Cook University
2020-2024
Australian Institute of Tropical Health and Medicine
2021-2024
Auburn University
2023
RTDS Technologies (Canada)
2004-2022
James Cook University Hospital
2021-2022
Public Health Scotland
2021
National Health and Medical Research Council
2021
Intensive Care National Audit & Research Centre
2021
University of Dundee
2021
Hundreds of human genes are associated with neurological diseases, but translation into tractable biological mechanisms is lagging. Larval zebrafish an attractive model to investigate genetic contributions diseases. However, current CRISPR-Cas9 methods difficult apply large screens studying behavioural phenotypes. To facilitate rapid screening, we developed a simple sequencing-free tool validate gRNAs and highly effective method capable converting >90% injected embryos directly F0...
Zebrafish tissues and cells have the unusual feature of not only containing a circadian clock, but also being directly light-responsive. Several zebrafish genes are induced by light, little is known about their role in clock resetting or mechanism which this might occur. Here we show that Cryptochrome 1a ( Cry1a ) plays key light entrainment clock. Intensity phase response curves reveal strong correlation between induction resetting. Overexpression studies acts as potent repressor function...
Light affects animal physiology and behavior more than simply through classical visual, image-forming pathways. Nonvisual photoreception regulates numerous biological systems, including circadian entrainment, DNA repair, metabolism, behavior. However, for the majority of these processes, photoreceptive molecules involved are unknown. Given diversity photophysiological responses, question arises whether a single photopigment or greater proteins within opsin superfamily detect photic stimuli....
Traditionally, circadian clocks have been thought of as a neurobiological phenomenon. This view changed somewhat over recent years with the discovery peripheral tissue oscillators. In mammals, however, suprachiasmatic nucleus (SCN) in hypothalamus still retains critical role central synchronizer biological timing. Zebrafish, contrast, always reflected more highly decentralized level clock organization, individual cells and tissues contain directly light responsive pacemakers. As consequence,...
In most organisms, light plays a key role in the synchronization of circadian timing system with environmental day–night cycle. Light pulses that phase-shift clock also induce expression period ( per ) genes vertebrates. Here, we report cloning zebrafish gene, zfper4 , which is remarkable being repressed by light. We have developed an vivo luciferase reporter assay for this gene cells contain light-entrainable clock. High-definition bioluminescence traces enabled us to accurately measure...
Most clock genes encode transcription factors that interact to elicit cooperative control of function. Using a two-hybrid system approach, we have isolated two different partners zebrafish (zf) CLOCK, which are similar the mammalian BMAL1 (brain and muscle arylhydrocarbon receptor nuclear translocator-like protein 1). The homologs, zfBMAL1 zfBMAL2, contain conserved basic helix–loop–helix-PAS (Period-Arylhydrocarbon receptor-Singleminded) domains but diverge in carboxyl termini, thus bearing...
Specific stages of the cell cycle are often restricted to particular times day because regulation by circadian clock. In zebrafish, both mitosis (M phase) and DNA synthesis (S clock-controlled in lines during embryo development. Despite ubiquitousness this phenomenon, relatively little is known about underlying mechanism linking clock cycle. study, we describe an evolutionarily conserved cell-cycle regulator, cyclin-dependent kinase inhibitor 1d (20 kDa protein, p20), which along with p21, a...
The circadian clock controls cell proliferation in a number of healthy tissues where renewal and regeneration are critical for normal physiological function. intestine is an organ that typically undergoes regular cycles division, differentiation apoptosis as part its role digestion nutrient absorption. aim this study was to explore regulation cycle gene expression the zebrafish intestine. Here we show gut contains directly light-entrainable pacemaker, which regulates daily timing mitosis....
The requirement for protein synthesis in the mechanism of a circadian pacemaker was investigated by using inhibitors synthesis. Continuous treatment ocular mollusc Bulla gouldiana with anisomycin or cycloheximide substantially lengthened (up to 39 and 52 hr, respectively) free-running period rhythm. To determine whether high concentrations inhibitor could stop pacemaker, long pulse treatments various durations 44 hr) were applied subsequent phase rhythm assayed. observed phases after...