A5087740990- Circadian rhythm and melatonin
- Photoreceptor and optogenetics research
- Genetics, Aging, and Longevity in Model Organisms
- Advanced Proteomics Techniques and Applications
- Marine Ecology and Invasive Species
- Subterranean biodiversity and taxonomy
- Adipose Tissue and Metabolism
- High Altitude and Hypoxia
- Malaria Research and Control
- Bayesian Methods and Mixture Models
- Light effects on plants
- stochastic dynamics and bifurcation
- Lipid Membrane Structure and Behavior
- Hemoglobin structure and function
- Data-Driven Disease Surveillance
- Spaceflight effects on biology
- Plant Molecular Biology Research
- Muscle metabolism and nutrition
- Mitochondrial Function and Pathology
- Sleep and related disorders
- Adhesion, Friction, and Surface Interactions
- Fungal and yeast genetics research
- Endoplasmic Reticulum Stress and Disease
- Biomedical and Engineering Education
- Molecular Communication and Nanonetworks
MRC Laboratory of Molecular Biology
2018-2025
The Francis Crick Institute
2023
University of Surrey
2017-2022
University of Cambridge
2019
Medical Research Council
2019
Healthcentric Advisors
2017
University College London
2010-2016
MRC Laboratory for Molecular Cell Biology
2013-2016
Instituto de Investigação Agrária de Moçambique
2015
London Centre for Nanotechnology
2015
The well-established negative health outcomes of sleep deprivation, and the suggestion that availability electricity may enable later bed times without compensating extension in morning, have stimulated interest studying communities whose pattern resemble a pre-industrial state. Here, we describe activity two neighbouring communities, one urban (Milange) rural (Tengua), region Mozambique where urbanisation is an ongoing process. differ amount timing daily light exposure, with bedtimes (≈1 h)...
SUMMARY Outbreaks of infectious diseases must be detected early for effective control measures to introduced. When dealing with large amounts data, automated procedures can usefully supplement traditional surveillance methods, provided that the wide variety patterns and frequencies infections are taken into account. This paper describes a robust system developed process weekly reports received at Communicable Disease Surveillance Centre. A simple regression algorithm is used calculate...
Abstract Optimum protein function and biochemical activity critically depends on water availability because solvent thermodynamics drive folding macromolecular interactions 1 . Reciprocally, macromolecules restrict the movement of ‘structured’ molecules within their hydration layers, reducing available ‘free’ bulk therefore total thermodynamic potential energy water, or potential. Here, concentrated solutions such as cytosol, we found that modest changes in temperature greatly affect...
The mammalian cryptochrome proteins (CRY1 and CRY2) are transcriptional repressors most notable for their role in circadian feedback. Not all rhythms depend on CRY proteins, however, the promiscuous interactors that also regulate many other processes. In cells with chronic deficiency, protein homeostasis is highly perturbed, a basal increase cellular stress activation of key inflammatory signalling pathways. Here, we developed tools to delineate specific effects reduction, rather than better...
Electrical correlates of the physiological state a cell, such as membrane conductance and capacitance, well cytoplasm conductivity, contain vital information about cellular function, ion transport across membrane, propagation electrical signals. They are, however, difficult to measure; gold-standard techniques are typically unable measure more than few cells per day, making widespread adoption limiting statistical reproducibility. We have developed dielectrophoretic platform using disposable...
Between 6-20% of the cellular proteome is under circadian control and tunes mammalian cell function with daily environmental cycles. For viability, to maintain volume within narrow limits, variation in osmotic potential exerted by changes soluble must be counterbalanced. The mechanisms consequences this compensation have not been investigated before. In cultured cells tissue we find that involves electroneutral active transport Na+, K+, Cl- through differential activity SLC12A family...
SUMMARY Insects possess adhesive organs that allow attachment to diverse surfaces. Efficient adhesion must be retained throughout their lifetime even when pads are exposed contamination. Many insects groom structures, but it is possible self-cleaning properties also play an important role. We measured forces of insect on glass after contamination with microspheres and found both smooth (stick insects: Carausius morosus) hairy (dock beetles: Gastrophysa viridula) exhibit self-cleaning....
Medicinal plants have contributed significantly to current malaria treatment. Emergence of resistance currently available drugs has necessitated the search for new plant-based anti-malarial agents and several plant-based, pharmacologically active compounds been isolated. This study was conducted validate traditional usage Echinops kebericho treating in health care system Ethiopia. The roots E. were collected from Masha Woreda, Sheka Zone. After collection, plant materials identified by a...
Cellular circadian rhythms confer temporal organisation upon physiology that is fundamental to human health. Rhythms are present in red blood cells (RBCs), the most abundant cell type body, but their physiological function poorly understood. Here, we a novel biochemical assay for haemoglobin (Hb) oxidation status which relies on redox-sensitive covalent haem-Hb linkage forms during SDS-mediated lysis. Formation of this lowest when ferrous Hb oxidised, form ferric metHb. Daily observed mouse...
Abstract Yeast physiology is temporally regulated, this becomes apparent under nutrient-limited conditions and results in respiratory oscillations (YROs). YROs share features with circadian rhythms interact with, but are independent of, the cell division cycle. Here, we show that minimise energy expenditure by restricting protein synthesis until sufficient resources stored, while maintaining osmotic homeostasis quality control. Although nutrient supply constant, cells sequester store...
Temperature compensation and period determination by casein kinase 1 (CK1) are conserved features of eukaryotic circadian rhythms, whereas the clock gene transcription factors that facilitate daily expression rhythms differ between phylogenetic kingdoms. Human red blood cells (RBCs) exhibit temperature-compensated which, because RBCs lack nuclei, must occur in absence a transcription-translation feedback loop. We tested whether temperature dependent on CKs RBCs. As with nucleated cell types,...
Abstract Studying communities at different stages of urbanisation and industrialisation can teach us how timing intensity light affect the circadian clock under real‐life conditions. We have previously described a strong tendency towards morningness in Baependi Heart Study, located small rural town Brazil. Here, we tested hypothesis that this is associated with early phase based on objective measurements (as determined by dim melatonin onset, DLMO, activity) exposure. also analysed well...
Abstract Even in nonexcitable cells, the membrane potential V m is fundamental to cell function, with roles from ion channel regulation, development, cancer metastasis. arises transmembrane concentration gradients; standard models assume homogeneous extracellular and intracellular concentrations, that only exists across has no significance beyond it. Using red blood we show this incorrect, or at least incomplete; detectable surface, modulating produces quantifiable consistent changes...
Most animals and plants live on the planet exposed to periods of rhythmic light dark. As such, they have evolved endogenous circadian clocks regulate their physiology rhythmically, non-visual detection mechanisms set clock environmental light-dark cycle. In case fish, pacemakers are not only present in majority tissues cells, but these themselves directly light-sensitive, expressing a wide range opsin photopigments. This broad sensitivity exists clock, also impacts fundamental cell...
Article29 November 2021Open Access Source DataTransparent process CRYPTOCHROMES promote daily protein homeostasis David C S Wong orcid.org/0000-0002-1712-9527 MRC Laboratory of Molecular Biology, Cambridge, UK Search for more papers by this author Estere Seinkmane orcid.org/0000-0002-3636-4709 These authors contributed equally to work Aiwei Zeng orcid.org/0000-0003-0354-2529 Alessandra Stangherlin orcid.org/0000-0001-7296-1183 Nina M Rzechorzek orcid.org/0000-0003-3209-5019 Andrew D Beale...
Abstract Circadian (∼24 h) rhythms are a fundamental feature of life, and their disruption increases the risk infectious diseases, metabolic disorders, cancer 1–6 . couple to cell cycle across eukaryotes 7,8 but underlying mechanism is unknown. We previously identified an evolutionarily conserved circadian oscillation in intracellular potassium concentration, [K + ] i 9,10 As critical events regulated by 11,12 , enticing hypothesis that form basis this coupling. used minimal model cell, alga...
Abstract Early mammals were nocturnal until the Cretaceous-Paleogene extinction facilitated their rapid expansion into daytime niches. Diurnality subsequently evolved multiple times, independently, but mechanisms facilitating this switch are unknown. We found that physiological daily temperature shifts oppositely affect circadian clock rhythms in versus diurnal mammals. This occurs through a cell-intrinsic signal inverter, mediated by global differences protein phosphorylation, and effected...
Summary Circadian rhythms result from cell-intrinsic timing mechanisms that impact health and disease 1,2 . To date, however, neural circadian research has largely focused on the hypothalamic circuitry of nocturnal rodents 3 Whether exist in human brain cells is unknown. Here we show bona fide neurons, glia, cerebral organoids, organoid slices (ALI-COs) 4–8 Human are synchronised by physiological cues such as glucocorticoids daily temperature cycles, these temperature-compensated across...