A5055291269- Circadian rhythm and melatonin
- Cellular transport and secretion
- Advanced Proteomics Techniques and Applications
- Time Series Analysis and Forecasting
- Parkinson's Disease Mechanisms and Treatments
- Retinal Development and Disorders
- Muscle metabolism and nutrition
- Plant Reproductive Biology
- Photonic and Optical Devices
- Quantum optics and atomic interactions
- Metabolomics and Mass Spectrometry Studies
- Cholinesterase and Neurodegenerative Diseases
- Gene Regulatory Network Analysis
- Neurobiology and Insect Physiology Research
- Genetics, Aging, and Longevity in Model Organisms
- Diabetes and associated disorders
- Influenza Virus Research Studies
- Genetics, Bioinformatics, and Biomedical Research
- Ecosystem dynamics and resilience
- Neural Networks and Reservoir Computing
MRC Laboratory of Molecular Biology
2023-2025
University of York
2019-2024
The mammalian cryptochrome proteins (CRY1 and CRY2) are transcriptional repressors most notable for their role in circadian feedback. Not all rhythms depend on CRY proteins, however, the promiscuous interactors that also regulate many other processes. In cells with chronic deficiency, protein homeostasis is highly perturbed, a basal increase cellular stress activation of key inflammatory signalling pathways. Here, we developed tools to delineate specific effects reduction, rather than better...
The within-host environment changes over circadian time and influences the replication severity of viruses. Genetic knockout transcription factors CRYPTOCHROME 1 2 ( CRY1 −/− / CRY2 ; CKO) leads to altered protein homeostasis chronic activation integrated stress response (ISR). adaptive ISR signalling pathways help restore cellular by downregulating synthesis in endoplasmic reticulum overloading or viral infections. By quantitative mass spectrometry analysis, we reveal that many recognition...
Background: Inherited mutations in the LRRK2 protein are common causes of Parkinson’s disease, but mechanisms by which increased kinase activity mutant leads to pathological events remain be determined. In vitro assays (heterologous cell culture, phospho-protein mass spectrometry) suggest that several Rab proteins might directly phosphorylated LRRK2-G2019S. An vivo screen expression dopaminergic neurons young adult Drosophila demonstrated a strong genetic interaction between LRRK2-G2019S and...
Rhythmic data are ubiquitous in the life sciences. Biologists need reliable statistical tests to identify whether a particular experimental treatment has caused significant change rhythmic signal. When these signals display nonstationary behaviour, as is common many biological systems, established methodologies may be misleading. Therefore, there real for new methodology that enables formal comparison of processes. As circadian behaviour best understood spectral domain, here we develop novel...
ABSTRACT Although costly to maintain, protein homeostasis is indispensable for normal cellular function and long-term health. In mammalian cells tissues, daily variation in global synthesis has been observed, but its utility consequences proteome integrity are not fully understood. Using several different pulse-labelling strategies, here we gain direct insight into the relationship between abundance proteome-wide. We show that degradation varies in-phase with synthesis, facilitating rhythms...
Abstract Inherited mutations in the LRRK2 protein are commonest known cause of Parkinson’s, but molecular link from increased kinase activity to pathological neurodegeneration remains be determined. In vitro (biochemical and cell culture) assays led hypothesis that several Rab GTPases might substrates. Here we show Rab10 potently modifies - G2019S mediated electrophysiological responses an vivo screen, which each was overexpressed Drosophila dopaminergic neurons. We therefore tested effect...
Abstract Background Inherited mutations in the LRRK2 protein are most common known cause of Parkinson’s, but mechanisms by which increased kinase activity mutant leads to pathological events remain be determined. In vitro assays (heterologous cell culture, phospho-protein mass spectrometry) suggest that several Rab proteins might directly phosphorylated LRRK2-G2019S . Which Rabs interact with dopaminergic neurons facilitate normal and physiological responses remains An vivo screen expression...
Abstract The nematode Caenorhabditis elegans is an unconventional model in chronobiology, reported to exhibit physiological and behavioural circadian rhythms while lacking a defined transcriptional oscillator. extent importance of C. remain uncertain, given probable lack functional conservation key proteins, relatively non-robust ambiguous diel ecology. Here, we investigated the temporal coordination gene expression post-development using RNA sequencing. Over time series, which synchronised...
Photonic qubits constitute a leading platform to disruptive quantum technologies due their unique low-noise properties. The cost of the photonic approach is non-deterministic nature many processes, including single-photon generation, which arises from parametric sources and negligible interaction between photons. Active temporal multiplexing - repeating generation process in time rerouting single modes using an optical switching network promising overcome this challenge will likely be...
Rhythmic data are ubiquitous in the life sciences. Biologists need reliable statistical tests to identify whether a particular experimental treatment has caused significant change rhythmic signal. When these signals display nonstationary behaviour, as is common many biological systems, established methodologies may be misleading. Therefore, there real for new methodology that enables formal comparison of processes. As circadian behaviour best understood spectral domain, here we develop novel...
Abstract Circadian behavioural deficits, such as increased daytime naps and reduced night-time sleep, are common in Alzheimer’s disease other tauopathies. But it has remained unclear whether these circadian abnormalities arise from tau pathology either the master pacemaker or downstream neurons. Here we study this question by selectively expressing different human proteins specific Drosophila brain circuits monitoring locomotor activity under light-dark (LD) “free-running” dark-dark (DD)...