A5060446533- Antimicrobial Resistance in Staphylococcus
- Oral microbiology and periodontitis research
- Bacteriophages and microbial interactions
- Dental Health and Care Utilization
- Bacterial Genetics and Biotechnology
- Dental Erosion and Treatment
- Bacterial biofilms and quorum sensing
- Endodontics and Root Canal Treatments
- RNA and protein synthesis mechanisms
- Biochemical and Structural Characterization
- Dental Radiography and Imaging
- Infective Endocarditis Diagnosis and Management
- Streptococcal Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Probiotics and Fermented Foods
- Antimicrobial Peptides and Activities
- Dental materials and restorations
- Advanced biosensing and bioanalysis techniques
- Salivary Gland Disorders and Functions
- Enterobacteriaceae and Cronobacter Research
- Advanced MRI Techniques and Applications
- Dental Implant Techniques and Outcomes
- Fluoride Effects and Removal
- Radioactive element chemistry and processing
- Bone Tissue Engineering Materials
University of Michigan
2003-2024
American Association of Endodontists
2010
Centers for Disease Control and Prevention
2003
J. Craig Venter Institute
2003
Biolog (United States)
2003
Gunma University
1992
Vancomycin is usually reserved for treatment of serious infections, including those caused by multidrug-resistant Staphylococcus aureus . A clinical isolate S. with high-level resistance to vancomycin (minimal inhibitory concentration = 1024 μg/ml) was isolated in June 2002. This harbored a 57.9-kilobase multiresistance conjugative plasmid within which Tn 1546 ( vanA ) integrated. Additional elements on the encoded trimethoprim dfrA ), β-lactams blaZ aminoglycosides aacA-aphD and...
The aim was to evaluate the effects of orally administered Lactobacillus rhamnosus GG (LGG) and Bifidobacterium animalis subsp. lactis BB-12 (BB-12) on number salivary mutans streptococci (MS), amount plaque, gingival inflammation oral microbiota in healthy young adults. study a randomised, controlled, double-blind trial. Healthy volunteers used lozenges containing combination LGG (test group, n = 29) or without added probiotics (control 31) for 4 weeks. At baseline at end test period,...
Background/aims: Enterococci have been implicated in persistent root canal infections but their role the infection process remains unclear. This study investigated virulence, phenotype and genotype of 33 endodontic enterococcal isolates. Methods: Phenotypic tests were conducted for antibiotic resistance, clumping response to pheromone, production gelatinase, hemolysin bacteriocin. Genotype analysis involved polymerase chain reaction amplification virulence determinants encoding aggregation...
Transposon Tn916 is a 16.4-kilobase, broad-host-range, conjugative transposon originally identified on the chromosome of Enterococcus (Streptococcus) faecalis DS16. Its termini have been sequenced along with junction regions for two different insertions. The ends were found to contain imperfect inverted repeat sequences identity at 20 26 nucleotides. Further in from ends, directly repeated present, 24 27 nucleotides matching. contained homologous segments but nature not consistent direct...
Vancomycin-resistant Enterococcus faecalis coisolated with vancomycin-resistant (VanA) Staphylococcus aureus was found to contain two plasmids, designated pAM830 (45 kb) and pAM831 (95 kb). pAM830, be conjugative closely related the Inc18 family of broad-host-range encodes resistances vancomycin (via a Tn1546-like element) erythromycin; gentamicin, streptomycin, erythromycin.
Tn916 [carries tet(M)] is a 16.4-kb conjugative transposon that can establish itself in multiple copies Enterococcus faecalis. To study the interaction of coresident homologous transposons during conjugation, an E. faecalis mutant defective recombination was utilized for construction strains harboring delta E (a derivative which erm substituted tet) on chromosome and nonconjugative plasmid. When these were used as donors, two able to transfer independently; however, they found become...
Aim: Antimicrobial and bioactive restorative materials are needed to develop a bacteria free environment tight bond with the surrounding tissue, preventing spread of secondary caries thus extending lifetime dental restorations. The characteristic properties new antibacterial composites presented in this work. have been microstructurally characterized both long short term studied. Methods: Ag-doped sol-gel derived glass (Ag-BG) was incorporated into resin composite concentrations 5, 10 15...
Summary The sex pheromone cAM373 of Enterococcus faecalis and the related staph ‐cAM373 Staphylococcus aureus were found to correspond heptapeptides located within C‐termini signal sequences putative prelipoproteins. deduced mature forms lipoproteins share no detectable homology presumably serve unrelated functions in cells. chromosomally encoded genetic determinants for production pheromones have been identified designated camE (encoding cAM373) camS ‐cAM373). Truncated full‐length clones...
Members of the Tn916 family conjugative transposons are able to insert themselves into Enterococcus faecalis hemolysin/bacteriocin plasmid pAD1 (and related elements) in such a way as generate hyperexpression hemolysin/bacteriocin. To examine this phenomenon more detail, E. (pAD1::Tn916) derivatives defective or altered hemolysin expression were isolated and characterized with respect production L (lytic) A (activator) component (also known CylA) specific location transposon. The mutants...
ABSTRACT Enterococcus faecalis strains secrete multiple peptides representing different sex pheromones that induce mating responses by bacteria carrying specific conjugative plasmids. The pheromone cAM373, which induces a response the enterococcal plasmid pAM373, has been of interest because similar activity is also secreted Streptococcus gordonii and Staphylococcus aureus . potential to facilitate intergeneric DNA transfer from E. concern extensive antibiotic resistance, including...
The vat(D) and erm(B) genes encoding streptogramin resistance in Enterococcus faecium transferred together, a direct physical link between was detected. Both the probes hybridized to fragments of different sizes donor transconjugants, which indicated transposition event.
The Enterococcus faecalis class IIa bacteriocin MC4-1 encoded by the sex pheromone-responding, multiple-antibiotic resistance plasmid pAMS1 exhibits "siblicidal" (sibling-killing) activity under certain conditions. Stabs of plasmid-containing cells on solid medium containing lawns bacteria same (plasmid-containing) strain give rise to zones inhibition. If host also produces gelatinase, cannot be detected.
The aim of this study was to evaluate the fluoride release from differently formulated 5% NaF varnishes into unstimulated whole saliva in vivo. concentration determined after application 3 different (5% NaF, + tricalcium phosphate [TCP], and amorphous calcium [ACP]) or a placebo. Fifteen subjects were recruited enrolled following Institutional Review Board approval based upon inclusion/exclusion criteria study. A cross-over design used for either one Unstimulated collected at baseline 1, 4,...