A5081162981- Glycosylation and Glycoproteins Research
- Cancer Research and Treatments
- Ubiquitin and proteasome pathways
- Endodontics and Root Canal Treatments
- Galectins and Cancer Biology
- Cancer, Hypoxia, and Metabolism
- Immunotherapy and Immune Responses
- Oral microbiology and periodontitis research
- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
- interferon and immune responses
- Cerebrospinal fluid and hydrocephalus
- Carbohydrate Chemistry and Synthesis
- Dental Radiography and Imaging
- Epigenetics and DNA Methylation
- Drilling and Well Engineering
- Infective Endocarditis Diagnosis and Management
- Nanoplatforms for cancer theranostics
- Genetic Syndromes and Imprinting
- RNA modifications and cancer
- Immune Cell Function and Interaction
- Advanced biosensing and bioanalysis techniques
- Prenatal Screening and Diagnostics
- Nanoparticle-Based Drug Delivery
- Radiation Therapy and Dosimetry
Nanostring Technologies (United States)
2020-2022
University of Washington
2020
University of Chicago
2015-2019
University of Illinois Chicago
2006-2013
Ashland (United States)
2012
University of Michigan
2004-2007
Abstract Aim The hypotheses tested in this study were that: (i) Enterococcus faecalis can survive long‐term entombment root filled teeth without additional nutrients, (ii) initial cell density influences the survival of E. instrumented canals and (iii) gelatinase‐production capacity canals. Methodology 150 extracted single canal to apical size 60 divided into six groups 25. Within each group 10 inoculated with either gelatinase‐producing OG1‐S other its gelatinase‐defective mutant OG1‐X....
Background/aims: Enterococci have been implicated in persistent root canal infections but their role the infection process remains unclear. This study investigated virulence, phenotype and genotype of 33 endodontic enterococcal isolates. Methods: Phenotypic tests were conducted for antibiotic resistance, clumping response to pheromone, production gelatinase, hemolysin bacteriocin. Genotype analysis involved polymerase chain reaction amplification virulence determinants encoding aggregation...
Emerging spatial profiling technology has enabled high-plex molecular in biological tissues, preserving the and morphological context of gene expression. Here, we describe expanding chemistry for Digital Spatial Profiling platform to quantify whole transcriptomes human mouse tissues using a wide range strategies sample types. We designed multiplexed situ hybridization probes targeting protein-coding genes transcriptomes, referred as or Whole Transcriptome Atlas (WTA). Human WTAs were...
To augment HIV-1 pox-protein vaccine immunogenicity using a next generation adjuvant, prime-boost strategy of recombinant modified vaccinia virus Ankara and multimeric Env gp145 was evaluated in macaques with either aluminum (alum) or novel liposomal monophosphoryl lipid A (MPLA) formulation adsorbed to alum, ALFA. Binding antibody responses were robust comparable between arms, while antibody-dependent neutrophil monocyte phagocytotic greatly enhanced by Per-exposure efficacy against...
The metabolic reprogramming associated with characteristic increases in glucose and glutamine metabolism advanced cancer is often ascribed to answering a higher demand for intermediates required rapid tumor cell growth. Instead, recent discoveries have pointed an alternative role metabolites as cofactors chromatin modifiers other protein posttranslational modification enzymes cells. Beyond epigenetic mechanisms regulating gene expression, many also modulate DNA repair, raising the question...
Cadmium free near-infrared AgInS<sub>2</sub>/ZnS quantum dots have been synthesized and used for imaging the microenvironment of cancer tumours.
Abstract Background The Dlk1 and Gtl2 genes define a region of mouse chromosome 12 that is subject to genomic imprinting, the parental allele-specific expression gene. Although imprinted play important roles in growth development, mechanisms by which imprinting established maintained are poorly understood. Differentially methylated regions (DMRs), carry methylation on only one allele, involved control at many loci. - contains three known DMRs, DMR 3' , intergenic 15 kb upstream promoter....
Current strategies to target tumors with nanomedicines rely on passive delivery via the enhanced permeability and retention effect, leveraging disorganized tumor microvasculature promote macromolecule extravasation reduced lymphatic venous drainage that favor retention. Nonetheless, FDA approvals clinical use of have lagged, reflecting failure display superiority over conventional formulations. Here, we exploited image-guided X-irradiation augment nanoparticle accumulation in tumors. A...
Abstract Emerging spatial profiling technology has enabled high-plex molecular in biological tissues, preserving the and morphological context of gene expression. Here we describe expanding chemistry for Digital Spatial Profiling platform to quantify whole transcriptomes human mouse tissues using a wide range strategies sample types. We designed multiplexed situ hybridization probe pools targeting protein-coding genes transcriptomes, hereafter referred as or Whole Transcriptome Atlas (WTA)....
The persistence of Enterococcus faecalis in treated root canals has been attributed to its resistance the high pH antimicrobial agents used during treatment, but specific mechanisms are not clear. We investigated survival and gene expression E. maintained alkaline media.E. JH2-2 was media at 7, 10, 11 12 either 25 degrees C or 37 for 1 week (168 h). At 24, 48, 72, 120 168 h, cell viability determined parallel with real-time quantitative polymerase chain reaction analyses stress response...
Radioresistance is a major hurdle in the treatment of head and neck squamous cell carcinoma (HNSCC). Here, we report that concomitant HNSCCs with radiotherapy mevalonate pathway inhibitors (statins) may overcome resistance. Proteomic profiling comparison radioresistant to radiosensitive revealed differential regulation biosynthetic pathway. Consistent this finding, inhibition by pitavastatin sensitized SQ20B cells ionizing radiation reduced their clonogenic potential. Overall, study...
<div>Abstract<p>The metabolic reprogramming associated with characteristic increases in glucose and glutamine metabolism advanced cancer is often ascribed to answering a higher demand for intermediates required rapid tumor cell growth. Instead, recent discoveries have pointed an alternative role metabolites as cofactors chromatin modifiers other protein posttranslational modification enzymes cells. Beyond epigenetic mechanisms regulating gene expression, many also modulate DNA...
<p>Time-lapse live cell imaging of caspase-3/7 activation in irradiated Panc02 cells treated with PUGNAc or alloxan</p>
<p>Three-dimensional imaging of irradiated MCF7 tumor xenografts with dual fluorescent reporters for IRIF (TagRFP, red) and shRNAmir expression (ZsGreen, green)</p>
<p>Intratumoral IRIF imaging demonstrates similar patterns for GFP-IBD (green) and TagRFP-IBD (red) foci in tumor at 24 h after irradiation with 6 Gy</p>