A5034037221- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Educational theories and practices
- Geography and Education Methods
- Gut microbiota and health
- Digital Economy and Work Transformation
- Developmental and Educational Neuropsychology
- Single-cell and spatial transcriptomics
- RNA and protein synthesis mechanisms
- Higher Education Teaching and Evaluation
- Click Chemistry and Applications
- Medical and Biological Sciences
- Bacterial Identification and Susceptibility Testing
- Employment and Welfare Studies
- E-Learning and Knowledge Management
- Education in Rural Contexts
- Oral microbiology and periodontitis research
- Clostridium difficile and Clostridium perfringens research
- Protein Degradation and Inhibitors
- Problem and Project Based Learning
- Histone Deacetylase Inhibitors Research
- Chromatin Remodeling and Cancer
Pfizer-University of Granada-Junta de Andalucía Centre for Genomics and Oncological Research
2017-2025
Instituto de Investigación Biosanitaria de Granada
2020-2025
Universidad de Granada
2017-2025
Hospital Universitario Virgen de las Nieves
2023-2025
Hospital Clínico Universitario Virgen de la Victoria
2023-2024
Centro de Investigación Biomédica en Red de Cáncer
2023-2024
Consejo Superior de Investigaciones Científicas
2024
Universidad de Sevilla
2024
Centro Andaluz de Biología Molecular y Medicina Regenerativa
2024
Biomedical Research Institute
2023
Scriptaid is a chemical compound with anti-tumoural effects due to its role as histone deacetylase inhibitor. Despite sharing part of the structure other ligands G-quadruplexes (G4s), interaction G4s has not been explored before. We synthesized and screened cytotoxic activity in cellular models colorectal cancer (CRC). extensively evaluated biological by cell cycle, immunofluorescence, qRT-PCR Western blot experiments. To identify G4 targets Scriptaid, we conducted panel binding assays....
Abstract Next generation sequencing methods are widely used in evaluating the structure and functioning of microbial communities, especially those centered on 16S rRNA subunit. Since Illumina Miseq, most platform, does not allow full gene, this study aims to evaluate whether choice different target regions might affect outcome microbiome studies regarding soil saliva samples. V1V3, V3V4, V4V5 V6V8 domains were studied, finding that while some showed differences detection certain bacterial...
Natural phenolic compounds have gained momentum for the prevention and treatment of cancer, but their antitumoral mechanism action is not yet well understood. In present study, we screened potential several in a cellular model colorectal cancer (CRC). We selected gallic acid (GA) as candidate terms potency selectivity extensively evaluated its biological activity. report on role GA ligand DNA G-quadruplexes (G4s), explaining effects, including transcriptional inhibition ribosomal CMYC genes....
DNA G-quadruplexes (G4s) are non-canonical secondary structures formed in guanine-rich sequences. Within the human genome, G4s found regulatory regions such as gene promoters and telomeres to control replication, transcription, telomere lengthening. In cellular context, there several proteins named G4-binding (G4BPs) that interact with G4s, either anchoring upon, stabilizing, and/or unwinding them. These may play different key roles regulation of endogenous G4 landscape its associated...
Analysis of circulating tumor DNA (ctDNA) is increasingly used for clinical decision-making in oncology. However, ctDNA could represent ≤ 0.1 % cell-free early-stage tumors and its detection requires high-sensitive techniques such as digital PCR (dPCR).
Early breast cancer patients often experience relapse due to residual disease after treatment. Liquid biopsy is a methodology capable of detecting tumor components in blood, but low concentrations at early stages pose challenges. To detect them, next-generation sequencing has promise entails complex processes. Exploring larger blood volumes could overcome detection limitations. Herein, total 282 high-volume plasma and blood-cell samples were collected for dual ctDNA/CTCs using single...
// Raquel Conde-Muiño 1, * , Carlos Cano 2, Victoria Sánchez-Martín 3, 4 Antonio Herrera 4, 5 Ana Comino 1 Pedro P. Medina Pablo Palma and Marta Cuadros Division of Colon & Rectal Surgery, University Hospital Virgen de las Nieves, Granada, Spain 2 Department Computer Science Artificial Intelligence, 3 Biochemistry, Molecular Biology III Immunology, GENYO, Centre for Genomics Oncological Research, Pfizer/University Granada/Junta Andalucía, PTS Biochemistry I, These authors have contributed...
Abstract Objective The safety, consequences, and dosage of long-term hormone therapy (HT) for postmenopausal women remain unclear. Our aim was to analyze the effects HT after 20 years in hysterectomy, focusing on symptoms menopause, blood pressure, lipid profiles, bone density. Methods A prospective observational longitudinal study designed. initial transdermal estradiol dose reduced half (0.025 mg/d) at 60 age. Different parameters including demographic, cardiovascular, density, metabolic...
Iron deficiency anemia (IDA) is a global public health concern affecting 1.6 billion people worldwide. The administration of iron supplements during the treatment IDA adversely affects intestinal barrier function and composition functionality microbiome, both which are already altered IDA. For this reason, it great interest to develop nutritional strategies aimed at alleviating these gut alterations associated with its treatment. In sense, fermented goat's milk (FGM) was studied due quality....
P. Sánchez 1M.I. Rodríguez 1C. Torres 1V. 2S. García-Rodríguez 1L.J. Martínez 1M.J. Álvarez 1R.M. Tapia-Haro 1A. Casas-Barrragán 1E. Aguilar-Ferrándiz 1L. Chica-Redecillas 1C.M. Morales-Álvarez 1M. Cuadros 1
M.I. Rodríguez 1M.J. Álvarez 1M. Cuadros 1S. García-Rodríguez 1V. Ayllon Sánchez 2L.J. Martínez 1P. 1C. Torres 1
Abstract Resveratrol (RSV) is one of the most studied and used biomolecules, for which many pharmacological effects targeting multiple tissues have been described. However, a common underlying mechanism driving its full activity has not elucidated to date. G-quadruplexes (G4s) are non-canonical nucleic acid structures found in promoters involved controlling gene transcription. This study demonstrates G4-dependent mode action RSV, explaining multi-target traits. RSV was shown stabilise...