A5075809391- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Bat Biology and Ecology Studies
- Sirtuins and Resveratrol in Medicine
- Free Radicals and Antioxidants
- Carbohydrate Chemistry and Synthesis
- Bacteriophages and microbial interactions
- Wildlife Ecology and Conservation
- HIV/AIDS drug development and treatment
- Primate Behavior and Ecology
- RNA and protein synthesis mechanisms
- Phytochemicals and Antioxidant Activities
- Enzyme Catalysis and Immobilization
- Amphibian and Reptile Biology
- Genomics and Phylogenetic Studies
- DNA Repair Mechanisms
- Glycosylation and Glycoproteins Research
- Species Distribution and Climate Change
- Analytical Chemistry and Chromatography
- Genetic diversity and population structure
- Evolution and Paleontology Studies
- Edible Oils Quality and Analysis
- Animal Behavior and Reproduction
- Neuroscience and Neuropharmacology Research
Hospital de Niños Superiora Sor María Ludovica
2023-2025
Instituto de Parasitología y Biomedicina "López - Neyra"
2015-2024
Northwestern University
2023-2024
Icesi University
2022-2023
Instituto Tecnológico Metropolitano
2023
Universidad de Granada
2020-2022
Bridge University
2022
Novartis Institutes for BioMedical Research
2020-2021
University of Bristol
2020-2021
University of Victoria
2020-2021
The physical and chemical factors that allow DNA to perform its functions in the cell have been studied for several decades. Recent advances synthesis manipulation of allowed this field move ahead especially rapidly during past fifteen years. One most common approaches study interactions involving has use base analogues which functional groups are added, deleted, blocked, or rearranged. Here we describe a different strategy, polar natural bases replaced by nonpolar aromatic molecules same...
We report the results of one first intrageneric analyses to simultaneously survey mitochondrial, Y‐chromosomal, and autosomal loci from same individuals representing taxa. Phylogenetic trees were constructed for each these genetic systems a pool 63 macaques, all 19 recognized species in this genus, eight outgroup The mitochondrial locus analyzed here (1.5 Kb) spans 3′ end 12S rDNA, tRNA‐VAL, 5′ 16S rDNA; Y chromosome dataset (3.1 consists genes SRY TSPY; two datasets include IRBP intron 3...
Abstract Scope : trans ‐Resveratrol (RES) and/(or) its metabolites exert many effects in vivo. Our aim was to study the metabolism and tissue distribution of RES using pig, a mammal physiologically close humans. Methods results Forty‐seven tissues, organs fluids were analyzed 6 h after intragastric administration (5.9 mg/kg body weight) HPLC‐MS/MS. Twelve seven dihydroresveratrol (DH‐RES) detected. DH‐RES main metabolite cecum, colon rectum, whereas RES‐3‐ O ‐glucuronide most abundant one...
Molecular phylogenetic relationships among all recognized species within the genusMacaca, were assessed using high-resolution restriction site mapping of mitochondrial ribosomal genes. By outgroup comparisons to other members cercopithecine subfamily, macaques appear be a monophyletic assemblage. Within genus, are in general consistent with previous genetic studies, though they less concordant separation into four distinct groups based on modification genitalia. Our data support: (1)Macaca...
Studies of the enzymatic mechanisms responsible for DNA synthesis and mutations have often focused on structural energetic issues base pair geometry Watson–Crick hydrogen (H) bonding.1 Until relatively recently, it was thought that a polymerase not likely to make specific H-bonded interactions with individual bases since four structures differ significantly, although earlier work pointed out H-bond acceptors appear at similar positions A–T G–C in minor groove.2 Recent X-ray crystal...
Quantitative descriptions of complex recognition processes rely on the measurement magnitude weak intermolecular interactions such as π-π interaction between arene units. In one approach thermodynamic cycles compounds with structural variations (mutations) are constructed. The association energy ΔG for edge-to-face in 1 was determined to be 1.4±0.8 kJ mol−1.
The effect of lipophilization the antioxidant efficiency hydroxytyrosol on fish oil enriched systems was studied. Hydroxytyrosol fatty acid esters with increasing size alkyl chain and different lipophilicity were tested in bulk oils oil-in-water emulsions. Results showed a significant activity both especially introduction lipophilic decreased effectiveness homogeneous as oils. In emulsion systems, presence short-medium (acetate, butyrate or octanoate) improved favoring physical location...
A fast, simple, and reliable method for the isolation determination of vitamins thiamin, riboflavin, niacin, pantothenic acid, pyridoxine, folic cyanocobalamin, ascorbic acid in food samples is proposed. The most relevant advantages proposed are simultaneous eight more common enriched products a reduction time required quantitative extraction, because consists merely addition precipitation solution centrifugation sample. Furthermore, this saves substantial amount reagents as compared with...
Our group has recently observed a nonlinear tendency in antioxidant capacity of different hydroxytyrosol fatty acid esters fish oil-in-water emulsions, where maximum efficiency appeared for octanoate. These results appear to disagree with the polar paradox. Because physical location antioxidants an oil−water interface been postulated as important factor explaining this behavior, we have prepared series tyrosol and chain length studied their surface-active properties water, because these...
The breast cancer resistance protein (BCRP/ABCG2) is a drug efflux transporter that can affect the pharmacological and toxicological properties of many molecules. Urolithins, metabolites produced by gut microbiota from ellagic acid (EA) ellagitannins, have been acknowledged with in vivo anti-inflammatory chemopreventive properties. This study evaluated whether urolithins (Uro-A, -B, -C, -D) their main phase II Uro-A sulfate, glucuronide, Uro-B glucuronide as well precursor EA were substrates...
This study reports the phylogenetic relationships of 13 species lasiurine bats using 4 loci from mitochondrial and Y-chromosomal DNA. Our results support a sister taxon relationship between red hoary bats, with yellow being more distantly related. Several taxonomic revisions are suggested based on this study. We recognition 3 separate genera: Lasiurus (red bats), Dasypterus (yellow Aeorestes (hoary including A. egregius which groups closely to than its traditional placement bats). recognize...
Among several commercial enzymes screened for chitosanolytic activity, Neutrase 0.8L (a protease from Bacillus amyloliquefaciens) was selected in order to obtain a product enriched deacetylated chitooligosaccharides (COS). The hydrolysis of different chitosans with this enzyme followed by size exclusion chromatography (SEC-ELSD), mass spectrometry (ESI-Q-TOF), and high-performance anion-exchange pulsed amperometric detection (HPAEC-PAD). converted 10 g/L various into mostly oligosaccharides,...
Abstract The polymorphic nature of G‐quadruplex (G4) DNA structures points to a range potential applications in nanodevices and an opportunity control G4 biological settings. Light is attractive means for the regulation oligonucleotide structure as it can be delivered with high spatiotemporal precision. However, surprisingly little attention has been devoted towards development ligands that allow photoregulation folding. We report novel G4‐binding chemotype derived from stiff‐stilbene....
G-quadruplexes (G4) are DNA secondary structures that take part in the regulation of gene expression. Putative G4 forming sequences (PQS) have been reported mammals, yeast, bacteria, and viruses. Here, we present PQS searches on genomes T. brucei, L. major, P. falciparum. We found telomeric new motifs. Biophysical experiments showed EBR1, a 29 nucleotide long highly repeated forms stable structure. ligands based carbohydrate conjugated naphthalene diimides (carb-NDIs) bind G4's including...
G‐quadruplexes (G4) are four‐stranded structures formed from guanine‐rich oligonucleotides. Their defined 3D and polymorphic nature set them apart classical nucleic acid morphology suggest a range of potential applications in the development functional materials. Meanwhile, occurrence G4 across genomes animals, plants pathogens suggests roles for these biology that may be exploited therapeutic effect. Hundreds ligands reported to bind sequences with high specificity affinity, but such can...
Abstract We present G4-iM Grinder, a system for the localization, characterization and selection of potential G4s, i-Motifs higher order structures. A robust highly adaptable search engine identifies all structures that fit user’s quadruplex definitions. Their biological relevance, in vitro formation probability presence known-to-form are then used as filters. The outcome is an efficient methodology helps select best candidates subsequent analysis or macroscopic genomic assessment. As proof...