A5035755522- Protease and Inhibitor Mechanisms
- Inflammatory mediators and NSAID effects
- Aortic aneurysm repair treatments
- Nitric Oxide and Endothelin Effects
- Aortic Disease and Treatment Approaches
- Cardiac Fibrosis and Remodeling
- Antiplatelet Therapy and Cardiovascular Diseases
- Receptor Mechanisms and Signaling
- Renin-Angiotensin System Studies
- Angiogenesis and VEGF in Cancer
- Apelin-related biomedical research
- Atherosclerosis and Cardiovascular Diseases
- Acute Kidney Injury Research
- Cardiac electrophysiology and arrhythmias
- Renal Diseases and Glomerulopathies
- Lipoproteins and Cardiovascular Health
- Natural Antidiabetic Agents Studies
- Peroxisome Proliferator-Activated Receptors
- Infectious Aortic and Vascular Conditions
- Renal and related cancers
- Blood Coagulation and Thrombosis Mechanisms
- Neuropeptides and Animal Physiology
- Advanced Proteomics Techniques and Applications
- Mast cells and histamine
- Metabolism and Genetic Disorders
Inserm
2014-2024
Sorbonne Université
2006-2024
Maladies rénales fréquentes et rares : des mécanismes moléculaires à la médecine personnalisée
2021-2024
Hôpital Tenon
2019-2024
Université Sorbonne Nouvelle
2021-2024
Université Paris 1 Panthéon-Sorbonne
2022
Université Paris Cité
2006-2020
Délégation Paris 7
2011-2017
Laboratoire de Recherche Vasculaire Translationnelle
2016-2017
Sorbonne Paris Cité
2011-2017
Background Abdominal Aortic Aneurysms (AAAs) represent a particular form of atherothrombosis where neutrophil proteolytic activity plays major role. We postulated that recruitment and activation participating in AAA growth may originate part from repeated episodes periodontal bacteremia. Methods Findings Our results show human was associated with Neutrophil Extracellular Trap (NET) formation the IntraLuminal Thrombus, leading to release cell-free DNA. Human samples were shown contain...
The therapeutic potential of low-molecular-weight (LMW) fucoidan, a sulfated polysaccharide extracted from brown seaweed devoid direct antithrombin effect, was investigated in vitro and model critical hindlimb ischemia rat. In results showed that LMW fucoidan enhanced fibroblast growth factor (FGF)-2-induced [(3)H]thymidine incorporation cultured rat smooth muscle cells. Intravenous injection rats significantly increased the stromal-derived (SDF)-1 level 1.2 +/- 0.1 to 6.5 0.35 ng/ml plasma....
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The spectral Doppler mitral flow pattern, alone or combined with tissue annulus velocity, can be used to predict left ventricular (LV) filling pressure in humans, whereas invasive hemodynamic measurements are still required the rat. This study was undertaken assess whether LV end-diastolic (LVEDP) estimated using echocardiography rat after myocardial infarction (MI). Thirty-seven rats (23 MI coronary artery ligation and 14 sham-operated rats) were evaluated 3 mo surgery echo-Doppler...
PGE2 has been shown to induce relaxations in precontracted human pulmonary venous preparations, while arteries this response was not observed. We investigated and characterized the prostanoid receptors which are activated by veins.Human veins were cut as rings set up organ baths presence of a TP antagonist. A pharmacological study performed using selective EP1-4 ligands. The cellular localization EP4 immunohistochemistry their corresponding transcripts also these vessels.PGE2 agonists...
CD163 is a macrophage receptor for haemoglobin–haptoglobin (Hb–Hp) complexes, responsible the clearance of haemoglobin. We hypothesized that production soluble (sCD163) may be due to proleolytic shedding membrane by neutrophil elastase, reported increased in culprit atherosclerotic plaques. analysed relationship between solubilization and elastase vitro, culture, ex vivo human plaque samples, vivo, plasma patients with coronary artery disease. Neutrophil was shown enhance decrease uptake...
AimsAbdominal aortic aneurysm (AAA) is a particular form of atherothrombotic disease characterized by the dilation wall and presence an intraluminal thrombus (ILT). The objective present study was to evaluate pro-oxidant properties ILT characterize anti-oxidant capacity high-density lipoproteins (HDLs).
Rationale: Neovascularization favors intraplaque hemorrhage and plaque rupture. Development of therapeutic strategies against atheromatous angiogenesis requires elucidation its initiating factors. Objective: We investigated the contribution smooth muscle cells (SMCs) atheroma-derived lipids to initiation atheroma-associated neoangiogenesis. Methods Results: Forty human aortic segments, each harvested from a different donor, were classified as healthy or bearing early lesions, including fatty...
Human internal mammary arteries (IMA) and saphenous veins (SV) are frequently used for coronary artery bypass graft surgery. Intra- postoperatively, the grafts exposed to inflammatory conditions, under which there is a striking increase in synthesis of prostaglandin E(2) (PGE(2) ). In this context, physiological response these vascular PGE(2) highly relevant. The aim study was thus characterize receptor subtypes (EP(1) , EP(2) EP(3) or EP(4) ) involved modulation tone two vessels.Rings IMA...
IntroductionDuring glomerular diseases, podocyte-specific pathways can modulate the intensity of histological disease and prognosis. The therapeutic targeting these could thus improve management prognosis kidney diseases. Janus Kinase/ Signal Transducer Activator Transcription (JAK/STAT) pathway, classically described in immune cells, has been recently detailed intrinsic cells.MethodsWe describe STAT5 expression human biopsies from patients with focal segmental glomerulosclerosis (FSGS)...
Abstract— Downregulation of the L-type Ca 2+ current ( I ) is an important determinant electrical remodeling diseased atria. Using a rat model heart failure (HF) due to ischemic cardiopathy, we studied in isolated left atrial myocytes with whole-cell patch-clamp technique and biochemical assays. density was markedly reduced (1.7±0.1 pA/pF) compared sham-operated rats (S) (4.1±0.2 pA/pF), but its gating properties were unchanged. Calcium channel α 1C -subunit quantities not significantly...
Tetracycline is a powerful tool for controlling the expression of specific transgenes (TGs) in various tissues, including heart. In these mouse systems, TG repressed/enhanced by adding doxycycline (Dox) to diet. However, Dox has been shown attenuate matrix metalloproteinase (MMP) and activity MMP inactivation mitigates left ventricular (LV) remodeling animal models heart failure. Therefore, we examined influence on LV mice after transverse aortic constriction (TAC). One month TAC, cardiac...
Hypertension represents one of the main risk factors for vascular diseases. Genetic susceptibility may influence rate its development and associated remodeling. To explore markers hypertension-related morbidity, we have used surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry to study changes in proteins released by aorta two rat strains with different susceptibilities hypertension. Fischer Brown Norway (BN) rats were divided into a control group...
Varicose veins are elongated and dilated saphenous veins. Despite the high prevalence of this disease, its pathogenesis remains unclear. Aims In study, we investigated control matrix metalloproteinases (MMPs) expression by prostaglandin (PG)E2 during vascular wall remodeling human varicose Methods Results (small (SDv) large diameter (LDv)) healthy (SV) were obtained after surgery. Microsomal cytosolic PGE-synthases (mPGES cPGES) protein mRNA responsible for PGE2 metabolism analyzed in all...
Haploinsufficiency of elastin leads, in more than half patients with Williams–Beuren syndrome, to development supravalvular aortic stenosis and hypertension. Determining mechanisms implicated synthesis would be interest find new elastogenic molecules treat such a pathology. Here, we analyzed the signaling pathway linking intracellular calcium concentration regulation able increase synthesis. Their ability was then investigated, vitro vivo, using inhibitors highlighted pathway. The Brown...
The use of cyclooxygenase-2 (COX-2) inhibitors has been reported to be associated with detrimental vascular events. aim our study was evaluate the role COX-2 activity in control human tone under inflammatory conditions. Using organ bath experiments, contraction induced by norepinephrine (NE), U46619, acetylcholine, and KCl performed on isolated internal mammary arteries (IMA) cultured presence or absence both interleukin-1β (IL-1β) lipopolysaccharide (LPS) without endothelium. Under these...
Chronic vascular rejection, the main cause of allograft failure, is characterized by destruction smooth muscle cells (SMCs) in media concomitantly with proliferation SMCs adjacent neointima. We hypothesized that alloantibodies might be responsible for these 2 opposite but coordinated events.We used rat aortic interposition model chronic rejection. During rejection process, a neointima composed proliferating from recipient developed, whereas media, all donor origin, underwent apoptosis....
The antihypertensive and diuretic effects of the aqueous extract Retama raetam Forssk. (RR) leaves were studied in both normotensive (WKY) spontaneously hypertensive rats (SHR). In SHR rats, daily oral administration RR (20 mg/kg) for three weeks exhibited a significant reduction blood pressure. systolic pressure decreased significantly from seventh day (P < 0.01) persisted through end treatment 0.001) rats. enhanced diuresis WKY 0.001). Furthermore, at dose 20 mg/kg produced increase on...
[18F]ML-10 (2-(5-fluoro-pentyl)-2-methylmalonic acid) is a positron emission tomography (PET) radiotracer that accumulates in cells presenting apoptosis-specific membrane alterations. The aim of this study was to test whether allows for the detection apoptotic located atherosclerotic plaques rabbits. Atherosclerotic were induced aortas five rabbits, and additional rabbits used as controls. Activity quantified vivo ex vivo. localization aortic wall identified by autoradiography. Average...
Chronic inhibition of nitric oxide (NO) synthesis by N(omega)-nitro-L-arginine methyl ester (L-NAME) induces hypertension associated with remodeling the arterial wall. In this study, we aimed at identifying genes and pathways involved in process aortic smooth muscle cells from Fischer 344 rats, which exhibit an accelerated after administration L-NAME.We studied transcriptional profile media 15 days (moderate hypertension) 30 (accelerated L-NAME (50 mg x kg(-1) d(-1)) using rat Affymetrix...
Low levels of low-density lipoprotein-cholesterol (LDL-C) are suspected to be associated with a risk hemorrhagic transformation after ischemic stroke. We assessed the cerebral ischemia/reperfusion in mice low LDL-C resulting from proprotein convertase subtilisin kexin 9 (PCSK9) deficiency.PCSK9-/- and PCSK9+/+ were fed high-fat/high-cholesterol (21%/0.15%) diet for 1 month. Plasma lipids measured using colorimetric assays. PCSK9-/- (n=15 per group) subjected 4-hour intraluminal occlusion...