Due to unique physico-chemical properties of –SH moiety thiols comprise widegroup biologically important compounds. A review devoted biological functions ofglutathione and phytochelatins with literature survey methods used analysis thesecompounds their interactions cadmium(II) ions Murashige-Skoog medium ispresented. For these purposes electrochemical techniques are used. Moreover, we revealedthe effect three different cadmium concentrations (0, 10 100 μM) on cadmiumuptake content in maize...

An Electrochemical Detection of Metallothioneins at the Zeptomole Level in Nanolitre VolumesWe report on improvement adsorptive transfer stripping technique (AdTS) coupled with differential pulse voltammetry Brdicka reaction to determine a thiol-protein. The current has been unable generate reproducible results when analyzing very low sample volumes (nanolitres). This obstacle can be overcome technically by modifying including cooling step adsorbed analyte. We tested determination promising...

Mesial temporal lobe epilepsy (mTLE) is a severe neurological disorder characterized by recurrent seizures. mTLE frequently accompanied neurodegeneration in the hippocampus resulting hippocampal sclerosis (HS), most common morphological correlate of drug resistance patients. Incomplete knowledge pathological changes mTLE+HS complicates its therapy. The mechanism underlying may involve abnormal gene expression regulation, including posttranscriptional networks involving microRNAs (miRNAs)....

The main aim of this work was to use multi-instrumental analytical apparatus toinvestigate the effects treatment with cadmium(II) and/or lead(II) ions (50, 250 and 500μM) for twelve days on early somatic spruce embryos (ESEs). Primarily we used imageanalysis estimation growth a fluorimetric sensor enzymatic detection ofviability treated ESEs. It follows from obtained results that Cd caused highertoxicity ESEs than Pb. Besides fundamental finding, observed grewand developed better in presence...

Abstract S100A11 (calgizzarin), a member of S100 family, is associated with several autoimmune diseases, including rheumatoid arthritis (RA). Neutrophil extracellular traps (NETs) are implicated in the pathogenesis RA and externalization some family members. Therefore, we aimed to determine association between NETs RA. For this purpose, levels NETosis markers were detected synovial fluid by immunoassays. The expression neutrophils tissue was assessed. Neutrophils isolated from peripheral...

Seizures elicited by corneal 6-Hz stimulation are widely acknowledged as a model of temporal lobe seizures. Despite the intensive research in rodents, no studies hint at this developing animals. We focused on seven age groups both male and female rats. Biphasic pulses with 0.3 ms duration current intensities from 20 to 80 mA were applied transcorneally for 3 s calculate threshold individual groups. Threshold intensity necessary elicitation clonic seizures was highly age- sex-dependent. The...

Abstract Objectives Interstitial lung disease (ILD) is the most frequent cause of mortality in patients with systemic sclerosis (SSc). Cartilage acidic protein-1 (CRTAC1), secreted by alveolar type-2 epithelial cells, a potential biomarker health. This study explores CRTAC1's role SSc, focusing on pulmonary involvement. Methods We collected plasma samples from 76 SSc (65 females, limited cutaneous (lc)SSc : 45, diffuse (dc)SSc 31, ILD 43, all fulfilled 2013 ACR/EULAR criteria) and 89 healthy...

In the present paper potato plants were cultivated in presence of ammonium sulphate or elemental sulphur supplementation into soil to reveal effects different forms on content nitrogen, phosphorus, potassium, calcium, magnesium and sulphur, yield tubers. During investigation influence tubers we did not observe significant changes. Average weight control per one experimental pot was 355 g. Application both resulted moderate reduction compared group; average value ranged from 320 350 g pot....

Treatment strategies for tumour diseases are progressively focusing on personalization of medicine. However, this focus requires methods revealing the early general biological mechanisms, including formation anti-cancer drugs' resistance. The low molecular mass protein metallothionein is thought to be crucial resistance in treatment based platinum-cytostatics. interactions between (MT) and cisplatin were determined by adsorptive transfer stripping technique coupled with differential pulse...

Temporal lobe epilepsy (TLE) is a severe neurological disorder accompanied by recurrent spontaneous seizures. Although the knowledge of TLE onset still incomplete, pathogenesis most likely involves aberrant expression microRNAs (miRNAs). miRNAs play an essential role in organism homeostasis and are widely studied as potential therapeutics biomarkers. However, many discrepancies discovered occur among studies due to model-specific miRNA expression, different ages patients, or...

Abstract Temporal lobe epilepsy (TLE) is the most common type. TLE onset in infancy aggravates features like severity, drug responsiveness, or development of comorbidities. These aggravations may arise from altered micro RNA (miRNA) expression specific to early disease. Although miRNA involvement widely studied, relationship between onset-age and has not been addressed. Here, we investigated profile infantile adult-onset rats combining sequencing PCR. Since changes with disease progression,...

Naphthoquinones are one of the groups secondary metabolites widespread innature, where they mostly appear as chromatic pigments. They embody broad-range ofbiological actions from phytotoxic to fungicidal. An anticancer effect naphthoquinonesstimulates an interest in determination and characterization single derivatives 1,2- and1,4-quinones biological samples. The main aim this work was suggest a techniquesuitable determine lawsone, juglone and/or plumbagin samples studyof their influence on...

Abstract Reduced glutathione (GSH) plays a number of key roles in many biochemical pathways. This peptide is highly reactive and forms conjugates with other molecules via its sulfhydryl moiety. The interactions the common heavy metal pollutant Cd(II) GSH were determined by using Brdicka reaction to evaluate whether this technique would be suitable as biomarker. After interaction ions, two characteristic changes measured voltammogram observed: Cat2 signal height decreased, new called P1 was...

Superporous poly(2-hydroxyethyl methacrylate-co-2-aminoethyl methacrylate) (P(HEMA-AEMA)) hydrogel scaffolds are designed for in vitro 3D culturing of leukemic B cells. Hydrogel porosity, which influences cell functions and growth, is introduced by adding ammonium oxalate needle-like crystals the polymerization mixture. To improve vitality, cell-adhesive Arg-Gly-Asp-Ser (RGDS) peptide immobilized on N-(γ-maleimidobutyryloxy)succinimide-activated P(HEMA-AEMA) hydrogels via reaction SH with...

Abstract The main aim of this paper is to study interaction between strontium and metallothionein (MT), determine changes in thiols (MT, reduced glutathione, cysteine, homocysteine) level plasma, serum, urine samples patients treated with ranelate (SrR). To investigate the interactions MT strontium(II) ions, adsorptive transfer stripping technique coupled differential pulse voltammetry (DPV) Brdicka reaction was employed. Besides standard signals (Co, RS 2 Co, Cat1, Cat2, Cat3), we observed...

Objective Osteoporosis is associated with an impaired balance between bone resorption and formation, which in turn leads to loss fractures. Many recent studies have underlined the regulatory role of microRNAs (miRNAs) remodeling processes their potential as biomarkers osteoporosis. The purpose this study was prospectively examine association circulating miRNAs estrogen status women before after oophorectomy, well oophorectomized on therapy. Methods In prospective study, we included 11...

IL-40 is a novel cytokine associated with autoimmune connective tissue disorders such as rheumatoid arthritis (RA) or Sjögren syndrome. We have previously shown an accumulation of in the RA joint and its expression by immune cells fibroblasts. Therefore, we aimed to assess role association hyaline cartilage chondrocyte activity. Immunohistochemistry was employed detect paired samples loaded unloaded regions osteoarthritis (OA) (n=5). Synovial fluid analysed ELISA OA (n=31) control...

Background: The development of hand osteoarthritis (HOA) and its progression into the erosive subset are unclear, but inflammation is suspected to be main source. To verify involvement in HOA pathogenesis, we evaluate serum inflammatory mediators their association with HOA-related clinical features patients. Methods: 153 participants (50 non-erosive patients, 54 49 healthy control subjects) were included this study. All patients underwent examination, which assessment tender swollen small...