A5033987725- DNA Repair Mechanisms
- Bacterial Genetics and Biotechnology
- DNA and Nucleic Acid Chemistry
- Bacteriophages and microbial interactions
- Polyomavirus and related diseases
- Sphingolipid Metabolism and Signaling
- Protein Structure and Dynamics
- Vascular Malformations Diagnosis and Treatment
- Immune cells in cancer
- Genomics and Chromatin Dynamics
- Phagocytosis and Immune Regulation
- Alzheimer's disease research and treatments
- Prion Diseases and Protein Misfolding
- Glioma Diagnosis and Treatment
- Caveolin-1 and cellular processes
- Inflammasome and immune disorders
- Extracellular vesicles in disease
- Intracerebral and Subarachnoid Hemorrhage Research
Second Affiliated Hospital of Zhejiang University
2025
University of Cambridge
2023
University of Southern California
2008-2014
The Ohio State University
2005
The minichromosome maintenance protein (MCM) complex is an essential replicative helicase for DNA replication in Archaea and Eukaryotes. Whereas the eukaryotic consists of 6 homologous proteins (MCM2–7), archaeon Sulfolobus solfataricus has only 1 MCM (ssoMCM), subunits which form a homohexamer. Here, we report 4.35-Å crystal structure near-full-length ssoMCM. shows elongated fold, with 5 subdomains that are organized into 2 large N- C-terminal domains. A ssoMCM hexamer generated based on...
The DNA tumor virus Simian 40 (SV40) is a model system for studying eukaryotic replication. SV40 large antigen (LTag) the initiator/helicase that essential genome LTag recognizes and assembles at viral replication origin. We determined structure of two multidomain subunits bound to origin DNA. reveals binding domains (OBDs) Zn AAA+ are involved in recognition assembly. Notably, OBDs recognize an unexpected manner. histidine residues insert into narrow minor groove region with enhanced...
DNA polymerase α-primase (Pol-prim) plays an essential role in eukaryotic replication, initiating synthesis of the leading strand and each Okazaki fragment on lagging strand. Pol-prim is composed a primase heterodimer that synthesizes RNA primer, subunit extends regulatory B-subunit (p68) without apparent enzymatic activity. thought to interact with replicative helicases, forming dynamic multiprotein assembly displays primosome At least three subunits physically hexameric helicase SV40 large...
Abstract The malignant interaction between tumor cells and immune is one of the important reasons for rapid progression refractoriness glioblastoma (GBM). As an essential metabolic center M2 macrophages, inhibition protein kinase RNA‐like endoplasmic reticulum (PERK) leads to reduction macrophages. Nevertheless, restriction blood‐brain barrier (BBB) non‐specific cell targeting hinder application PERK inhibitors in GBM. Herein, optimal NP‐M‐M2pep developed successfully, which has shown...
Unabated activation of the NLR family pyrin domain-containing 3 (NLRP3) inflammasome is linked with pathogenesis various inflammatory disorders. Polo-like kinase 1 (PLK1) has been widely studied for its role in mitosis. Here, using both pharmacological and genetic approaches, we demonstrate that PLK1 promoted NLRP3 at cell interphase. Using an unbiased proximity-dependent biotin identification (Bio-ID) screen interactome macrophages, show enhanced proximal association upon activation. We...