A5066540747- Nicotinic Acetylcholine Receptors Study
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
- Avian ecology and behavior
- Wildlife Ecology and Conservation
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- bioluminescence and chemiluminescence research
- Cholinesterase and Neurodegenerative Diseases
- COVID-19 Clinical Research Studies
- Insect and Pesticide Research
- Ecology and Vegetation Dynamics Studies
- Rangeland and Wildlife Management
- Species Distribution and Climate Change
- Orthopaedic implants and arthroplasty
- SARS-CoV-2 and COVID-19 Research
- Marine animal studies overview
- Long-Term Effects of COVID-19
- Fish Ecology and Management Studies
- Animal Behavior and Reproduction
- Photochromic and Fluorescence Chemistry
- Neurobiology and Insect Physiology Research
- Animal Ecology and Behavior Studies
- Sepsis Diagnosis and Treatment
- Phenothiazines and Benzothiazines Synthesis and Activities
SUNY College of Environmental Science and Forestry
2016-2025
Purchase College
2015-2025
Hôpital Foch
2021-2025
York University
2015-2024
State University of New York
2015-2024
Brighton and Sussex Medical School
2002-2024
Institute of Accelerating Systems and Applications
2022-2024
Ariadne Diagnostics (United States)
2024
American Society of Anesthesiologists
2024
Society of Cardiovascular Anesthesiologists
2024
After alkaline extraction, purified subsynaptic fragments isolated from Torpedo electric tissue exhibit on sodium dodecyl sulfate/polyacrylamide gel electrophoresis predominant peptides of apparent Mr 41,000, 50,000, and 65,000 (i.e., the characteristic nicotinic receptor in detergent solutions). The peptide 43,000 that is also found postsynaptic membranes recovered supernatant after extraction. alkaline-extracted were functionally intact, as demonstrated by following criteria. kinetics...
<h3>Importance</h3> Acutely ill inpatients with COVID-19 typically receive antithrombotic therapy, although the risks and benefits of this intervention among outpatients have not been established. <h3>Objective</h3> To assess whether anticoagulant or antiplatelet therapy can safely reduce major adverse cardiopulmonary outcomes symptomatic but clinically stable COVID-19. <h3>Design, Setting, Participants</h3> The ACTIV-4B Outpatient Thrombosis Prevention Trial was designed as a...
It is unknown whether ivermectin, with a maximum targeted dose of 600 μg/kg, shortens symptom duration or prevents hospitalization among outpatients mild to moderate COVID-19.
General anesthetics, including etomidate, act by binding to and enhancing the function of GABA type A receptors (GABA Rs), which mediate inhibitory neurotransmission in brain. Here, we used a radiolabeled, photoreactive etomidate analog ([ 3 H]azietomidate), retains anesthetic potency vivo enhances R vitro , identify directly, for first time, amino acids that contribute site. For Rs purified affinity chromatography from detergent extracts bovine cortex, [ H]azietomidate photoincorporation...
The competitive nicotinic antagonist d-[3H]tubocurarine was used as a photoaffinity label for the acetylcholine binding sites on receptor (AcChoR) from Torpedo. Irradiation with 254-nm UV light of AcChoR-rich membranes equilibrated resulted in covalent incorporation into alpha, gamma, and delta subunits that could be blocked by alpha-bungarotoxin or carbamoylcholine. concentrations required half-maximal specific gamma were 40 nM 0.9 microM, respectively, consistent dissociation constants...
Studies are presented of the equilibrium binding [3H]-d-tubocurarine (dTC) and [3H]acetylcholine (AcCh) to Torpedo postsynaptic membranes. The saturable [3H]dTC is characterized by two affinities: Kd1 = 33 +/- 6 nM Kd2 7.7 4.6 microM, with equal numbers sites. Both components completely inhibited pretreatment excess alpha-bungarotoxin or 100 microM nonradioactive dTC competitively carbamylcholine a KI nM, but not affected local anesthetics dimethisoquin, proadifen, meproadifen. biphasic...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIdentifying the Lipid-Protein Interface of Torpedo Nicotinic Acetylcholine Receptor: Secondary Structure ImplicationsMichael P. Blanton and Jonathan B. CohenCite this: Biochemistry 1994, 33, 10, 2859–2872Publication Date (Print):March 15, 1994Publication History Published online1 May 2002Published inissue 15 March 1994https://pubs.acs.org/doi/10.1021/bi00176a016https://doi.org/10.1021/bi00176a016research-articleACS PublicationsRequest reuse...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMapping of the acetylcholine binding site nicotinic receptor: [3H]nicotine as an agonist photoaffinity labelRichard E. Middleton and Jonathan B. CohenCite this: Biochemistry 1991, 30, 28, 6987–6997Publication Date (Print):July 16, 1991Publication History Published online1 May 2002Published inissue 16 July 1991https://pubs.acs.org/doi/10.1021/bi00242a026https://doi.org/10.1021/bi00242a026research-articleACS PublicationsRequest reuse...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPermeability control by cholinergic receptors in Torpedo postsynaptic membranes: agonist dose-response relations measured at second and millisecond timesRichard R. Neubig Jonathan B. CohenCite this: Biochemistry 1980, 19, 12, 2770–2779Publication Date (Print):June 10, 1980Publication History Published online1 May 2002Published inissue 10 June 1980https://pubs.acs.org/doi/10.1021/bi00553a036https://doi.org/10.1021/bi00553a036research-articleACS...
Highly purified cholinergic postsynaptic membranes from Torpedo electric tissue contain, in addition to the acetylcholine receptor (AcChoR), major proteins of Mr 43,000 and approximately 90,000 minor that can be removed by alkaline treatment. We have prepared an antiserum these alkaline-extractable reacts with protein but not any other membrane proteins, including AcChoR subunits. Immunofluorescent staining sections shows this binds innervated uninnervated surface electrocytes. In rat...
The kinetics of binding [3H]acetylcholine (AcCh) and [3H]carbamoylcholine (Carb) to membrane-bound nicotinic receptor from Torpedo electric tissue have been measured on the second time scale by rapid mixing ultrafiltration. concentration dependence association agonist ligand dissociation reisomerization following removal are analyzed in terms a model which observed is single population receptors that exists absence two interconvertible conformations, one weakly (R1) other with high affinity...
Twenty-six cases of dermatomyositis in children with follow-up studies ranging from one to 19 years are presented order indicate the natural history this disease. Of these 26 children, 10 dead, 4 still have active disease, crippling contractures and 8 able lead an normal life. Thirteen received endocrine therapy adrenocorticotropic hormone, cortisone or testosterone. This appeared produce at least symptomatic benefit some cases, particularly early course disease and, coordinated care, helped...
In past years, scientific discussion of the problem causal osteogenesis was dominated by conflict between osteoblast theory and induction theory. The work Orell Levander in particular contributed evidence to both theories. present series experiments, important osteogenetic significance soft-tissue components bone—periosteum, marrow, living cells bone canals—has been established anew. Even adults, latent were brought proliferation function within several days activating influence necrosis. To...
Abstract Regrowth of forests across the northeastern United States in recent decades has allowed for range expansion many forest‐dependent mammals region. However, these contemporary have smaller patch sizes, putting humans closer proximity to previously remote forested areas, and different species compositions structure compared historical forests. These conditions pose an increased risk exposure anthropogenic disturbances contaminants forest‐dwelling wildlife. We evaluated relationship...
Rapsyn, a peripheral membrane protein of skeletal muscle, clusters nicotinic acetylcholine receptors (nAChRs) at high density in the postsynaptic membrane. The mechanism nAChR clustering by rapsyn was analyzed expressing nAChRs HEK293T cells with various fragments mouse fused to green fluorescent protein. Membrane targeting is conferred solely its acylated N terminus, as myristoylated N-terminal 15 amino acids are sufficient target plasma However, neither myristoylation nor conserved acid...
Experimental Procedures" and Tables I-IV) are presented in miniprint at the end of this paper.Miniprint is easily read with aid a standard magnifying glass.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMapping the lipid-exposed regions in Torpedo californica nicotinic acetylcholine receptorMichael P. Blanton and Jonathan B. CohenCite this: Biochemistry 1992, 31, 15, 3738–3750Publication Date (Print):April 21, 1992Publication History Published online1 May 2002Published inissue 21 April 1992https://doi.org/10.1021/bi00130a003RIGHTS & PERMISSIONSArticle Views123Altmetric-Citations109LEARN ABOUT THESE METRICSArticle Views are COUNTER-compliant sum of...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTConformations of Torpedo acetylcholine receptor associated with ion transport and desensitizationRichard R. Neubig, Norman D. Boyd, Jonathan B. CohenCite this: Biochemistry 1982, 21, 14, 3460–3467Publication Date (Print):July 1, 1982Publication History Published online1 May 2002Published inissue 1 July 1982https://pubs.acs.org/doi/10.1021/bi00257a032https://doi.org/10.1021/bi00257a032research-articleACS PublicationsRequest reuse permissionsArticle...