A5045462591- Osteoarthritis Treatment and Mechanisms
- Cellular Mechanics and Interactions
- Single-cell and spatial transcriptomics
- Congenital heart defects research
- 3D Printing in Biomedical Research
- Zebrafish Biomedical Research Applications
- Developmental Biology and Gene Regulation
- Congenital gastrointestinal and neural anomalies
- RNA Research and Splicing
- Axon Guidance and Neuronal Signaling
- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- Tendon Structure and Treatment
- Cancer-related molecular mechanisms research
- Cell Adhesion Molecules Research
- Nerve injury and regeneration
- dental development and anomalies
- Neuroblastoma Research and Treatments
- Genetic Mapping and Diversity in Plants and Animals
- Evolution and Genetic Dynamics
- Infant Nutrition and Health
- Intestinal Malrotation and Obstruction Disorders
- Pancreatic function and diabetes
- Muscle Physiology and Disorders
- Animal Ecology and Behavior Studies
Karolinska Institutet
2019-2024
Medical University of Vienna
2023
University of Nebraska Medical Center
2017-2018
Abstract Formation of oriented myofibrils is a key event in musculoskeletal development. However, the mechanisms that drive myocyte orientation and fusion to control muscle directionality adults remain enigmatic. Here, we demonstrate developing skeleton instructs directional outgrowth skeletal other soft tissues during limb facial morphogenesis zebrafish mouse. Time-lapse live imaging reveals early craniofacial development, myoblasts condense into round clusters corresponding future groups....
Abstract Glial cells have been proposed as a source of neural progenitors, but the mechanisms underpinning neurogenic potential adult glia are not known. Using single cell transcriptomic profiling, we show that enteric glial represent state attained by autonomic crest they transition along linear differentiation trajectory allows them to retain while acquiring mature functions. Key loci in early nervous system progenitors remain open chromatin configuration glia, thus facilitating neuronal...
Hyaline cartilage is a specialized type of connective tissue that lines many moveable joints (articular cartilage) and contributes to bone growth (growth plate cartilage). composed single cell type, the chondrocyte, which produces unique hydrated matrix resist compressive stress. Although stress has profound effects on transcriptional networks biosynthesis in chondrocytes, mechanistic relationships between strain, signal transduction, metabolism, production remain superficial. Here, we...
Cartilaginous structures are at the core of embryo growth and shaping before bone forms. Here we report a novel principle vertebrate cartilage that is based on introducing transversally-oriented clones into pre-existing cartilage. This mechanism uncouples lateral expansion curved cartilaginous sheets from control thickness, process which might be evolutionary underlying adaptations facial shape. In rod-shaped (Meckel, ribs skeletal elements in developing limbs), transverse integration clonal...
Defining the final size and geometry of engineered tissues through precise control scalar vector components tissue growth is a necessary benchmark for regenerative medicine, but it has proved to be significant challenge engineers. The plate cartilage that promotes elongation long bones good model system studying morphogenetic mechanisms because composed single cell type, chondrocyte; chondrocytes are readily maintained in culture; trajectory predominately vector. In this cartilage, generated...
Abstract The sympathetic nervous system controls bodily functions including vascular tone, cardiac rhythm, and the “fight-or-flight response”. Sympathetic chain ganglia develop in parallel with preganglionic motor nerves extending from neural tube, raising question of whether axon targeting contributes to formation. Using nerve-selective genetic ablations lineage tracing mouse, we reveal that nerve-associated Schwann cell precursors (SCPs) contribute neurons satellite glia after initial...
Abstract How neurogenesis and gliogenesis are coordinated during development why mature glial cells often share properties with neuroectodermal progenitors remains unclear. Here, we have used single cell RNA sequencing to map the regulatory landscape of neuronal differentiation in mammalian enteric nervous system (ENS). Our analysis indicates that neurogenic trajectories branch directly from a linear gliogenic axis defined by autonomic neural crest adopting sequential states as they...
Mechanical stimuli are known to modulate biological functions of cells and tissues. Recent studies have suggested that compressive stress alters growth plate cartilage architecture results in modulation long bones children. To determine the role bone growth, we created a microfluidic device actuated by pneumatic pressure, dynamically (or statically) compress chondrocytes embedded alginate hydrogel cylinders. In this article, describe detailed methods for fabricating characterizing device....
Abstract Embryogenesis is commonly viewed through a tree model of cell differentiation, which does not adequately represent the spatiotemporal modulation multipotency underlying morphogenesis. Here we develop an integrated approach, combining in vivo single-cell high-throughput clonal lineage tracing with machine learning, to systematically decompose continuous spectra fate biases mouse embryos traced from neurulation until mid-gestation. The reconstructed patterns variation uncovered gene...
Mechanical stimuli are known to modulate biological functions of cells and tissues. Recent studies have suggested that compressive stress alters growth plate cartilage architecture results in modulation long bones children. To determine the role bone growth, we created a microfluidic device actuated by pneumatic pressure, dynamically (or statically) compress chondrocytes embedded alginate hydrogel cylinders. In this article, describe detailed methods for fabricating characterizing device....
Abstract Development of the nervous system is underpinned by changes in gene expression and chromatin configuration during embryogenesis early postnatal life. To uncover molecular mechanisms orchestrating cell lineage differentiation maturation enteric (ENS), we profiled transcriptome accessibility autonomic neural crest cells (ANCCs) their progeny mouse gut at key developmental stages adulthood. Integrative analyses defined a trajectory delineated ENS progenitors transitioning development...
ABSTRACT The sympathetic nervous system controls a wide spectrum of bodily functions including operation vessels, cardiac rhythm, and the “flight or fight response”. Sympathetic neurons, which are neural crest-derived, develop in coordination with presynaptic motor nerves extending from central (CNS). By using nerve-selective genetic ablations, we revealed that ganglia development depends on CNS-derived innervation. In absence preganglionic nerves, trunk chain were fragmented smaller size,...
Spatial and temporal regulation of chondrocyte maturation in the growth plate drives long bone growth. A notable feature cartilage is progression progenitor resting chondrocytes into clonal columns transit-amplifying proliferative chondrocytes. Following division chondrocytes, daughter cell pairs undergo a unique 90-degree rearrangement to form columns. Our previous studies using murine fluorescent membrane reporter system time-lapse imaging along with chemical inhibition adhesion suggested...