A5087425347- Plasma Applications and Diagnostics
- Cancer Cells and Metastasis
- Plasma Diagnostics and Applications
- Surface Modification and Superhydrophobicity
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Cancer-related Molecular Pathways
- Pancreatic and Hepatic Oncology Research
- Cancer Research and Treatments
- Glycosylation and Glycoproteins Research
- Electrohydrodynamics and Fluid Dynamics
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Pancreatic function and diabetes
- T-cell and B-cell Immunology
- Vector-borne infectious diseases
- Inhalation and Respiratory Drug Delivery
- Viral Infections and Vectors
- Blood properties and coagulation
- Proteoglycans and glycosaminoglycans research
- IL-33, ST2, and ILC Pathways
- Dust and Plasma Wave Phenomena
- COVID-19 and healthcare impacts
- Skin and Cellular Biology Research
- NF-κB Signaling Pathways
- Dermatological and Skeletal Disorders
Chiba University
2019-2025
National Institute of Advanced Industrial Science and Technology
2008-2021
Aichi Cancer Center
2006-2008
Nagoya University
2006
Scripps Research Institute
2004
Kansai Medical University
1988-1990
Massachusetts Institute of Technology
1933
University of Michigan
1933
Abstract The lymphoid tissue in the omentum, at so-called milky spots, is known as an initial place for disseminated cancer cells to develop into solid tumors. In present study, i.p. macrophages significantly took up oligomannose-coated liposomes (OMLs) that were injected peritoneal cavity, and then gradually accumulated omentum other tissues within 24 hours of injection OMLs. When 5-fluorouracil (5-FU) was encapsulated OMLs, >60% administered 5-FU omentum. Treatment 39°C 30 minutes...
The objective of this study is to determine whether there a pathway different from the natural blood coagulation process involved in plasma‐induced activation platelets and factors. Plasma treatment bleeding wounds generated glomerular structures, which could be detected high‐magnification scanning electron microscopy images, plasma flares produced aggregation on surface solutions containing bovine serum albumin, ovalbumin (OVA), or similar protein material. Ultramicroscopic analysis...
The formulas for the interaction between two identical atoms with an outer $s$-electron of arbitrary quantum number as developed by one writers (Rosen) in several earlier papers, are evaluated numerically. results presented form graphs which show shape Coulomb, exchange, and singlet energy curves various values how their minima vary latter. Curves from fundamental molecular constants may be read included. A table calculated hydrogen alkali-metal molecules is comparison made experimental...
The mortality of coronavirus disease 2019 (COVID-19) is strongly correlated with pulmonary vascular pathology accompanied by severe acute respiratory syndrome 2 (SARS-CoV-2) infection–triggered immune dysregulation and aberrant activation platelets. We combined histological analyses using field emission scanning electron microscopy energy-dispersive X-ray spectroscopy the lungs from autopsy samples single-cell RNA sequencing peripheral blood mononuclear cells to investigate pathogenesis...
Gastric cancer metastasised to the liver was found overexpress HER2 at a significantly higher incidence than primary gastric cancers. The purpose of present study investigate possibility molecular therapy targeting overexpression in metastasis. We developed three new HER2-overexpressing cell lines (GLM-1, GLM-2, GLM-4) without epidermal growth factor receptor (EGFR) mutations derived from such metastasis, two which had gene amplifications. All these GLM series were highly sensitive gefitinib...
A method for blood coagulation using low-energy atmospheric-pressure plasma (LEAPP) is confirmed as an alternative procedure to reduce tissue damage caused by heat. Blood LEAPP behaves differently depending on working gas species; helium more effective than argon in promoting fast coagulation. To analyse the difference reactive species produced and plasma, spectroscopic measurements were conducted without with a target material. compare emissions, experiments both plasmas performed under...
β1,4-N-acetylgalactosaminyltransferase III (β4GalNAc-T3), which was recently cloned and identified, exhibits GalNAc transferase activity toward a GlcNAcβ residue with β1,4-linkage, forming the N,N′-diacetyllactosediamine, GalNAcβ1,4GlcNAc (LacdiNAc or LDN). Though LacdiNAc has not been found in gastric mucosa, large amount of transcript detected our previous study. To increase knowledge β4GalNAc-T3 expression its product LacdiNAc, we examined exact localization human mucosa using newly...
Abstract To understand the mechanism of turbulent enhancement phenomena a neutral gas flow containing plasma ejected from nozzle equipment, schlieren optical method was performed to visualize behavior. It confirmed that starting point became closer exit, as amplitude discharge voltage (electric field) increased. study effect electric field on enhancement, two sets external electrodes were arranged in parallel, and allowed between upper lower electrodes. found bent, bending angle increased...
In a low-temperature atmospheric pressure plasma jet using helium gas, emission-propagation phenomena, such as streamers and striations were measured high-speed intensified charge-coupled device camera. A particular focus was placed on the study of dependence phenomena distance between nozzle target plate. When decreased, transition from positive streamer to spatially continuous emission resulted. further reduction resulted in new propagation mode which negative appeared alternately with...
Striation phenomena in a plasma flare produced by an atmospheric pressure jet had been observed. In this study, we measured the striation structure neon optical emission spectroscopy. As result, it shows that from is synchronized with current. We discuss temperature estimated Boltzmann plot using two lines of Ne I at 692.9 and 724.5 nm whose upper levels are [3p (2p6)] (2p10)], respectively. This has peaks spatial interval about 0.5 mm, peak positions correspond to variation intensity. also...
Abstract Tube-forming growth is an essential histological feature of pancreatic duct adenocarcinoma (PDAC) and the epithelium; nevertheless, nature signals that start to form tubular structures remains unknown. Here, we showed clonal PDAC cell lines in a three-dimensional (3D) culture experiment modeled PDAC. At beginning this study, isolated sphere- tube-forming clones from established mouse cancer via limiting dilution using collagen gel. Compared with cells spherical structures, formed...
The premalignant lesions of pancreatic cancer, intraepithelial neoplasia (PanIN), have a high frequency mutations in Kirsten rat sarcoma viral oncogene homologue (KRAS), and genetic alterations the retinoblastoma (Rb)-E2 factor (E2F) transformed 3T3 cell double minute 2 (MDM2)-p53 pathways accelerate development ductal adenocarcinoma. oncoprotein SV40 large T antigen (TAg) can inhibit effects Rb family molecules p53 on these pathways, targeted expression TAg mouse pancreas is associated with...
Low energy ionized gas coagulation equipment (LE-IGCE) using low temperature nonthermal atmospheric pressure plasmas has been attracting special attention with the popularization of minimally invasive surgery. In this work, thermal characteristics a commercial high (HE-IGCE) and specially designed LE-IGCE have studied an infrared camera optical emission spectroscopy during their treatments. The four substrates used for treatments were pork meats, copper (Cu) plates, wet tissues, glass...
Abstract To understand the aggregation mechanism of serum protein dispersed in solution distilled water induced by a low temperature atmospheric pressure plasma, we compared relationship between amount and experimental condition dependencies such as gas species, flow rate, distance plasma device solution. In this experiment, pure argon, helium, and/or mixture helium argon gases were used. From statistical analyses various conditions, it was found that monotonic supply total absolute charge...
Abstract In bronchial asthma patients, mucous cell metaplasia (MCM) and fibrosis occur in the epithelium interstitium, respectively. The mucus collagen fibers are identified by Periodic acid-Schiff stain (PAS) or Sirius red on optical microscopy. On a scanning electron microscope (SEM) observation, formalin-fixed-paraffin-embedded specimens have high insulation, thereby attenuating scattered signals leading to insufficient contrast. Moreover, there were no staining methods for SEM which...
Glycosaminoglycan (GAG) is a polysaccharide present on the cell surface as an extracellular matrix component, and composed of repeating disaccharide units consisting amino sugar uronic acid except in case keratan sulfate. Sulfated GAGs, such heparan sulfate, heparin, chondroitin sulfate mediate signal transduction growth factors, their functions vary with type degree sulfated modification. We have previously identified human mouse cochlins proteins that bind to GAGs. Here, we prepared...