A5038600971- Nuclear Structure and Function
- RNA Research and Splicing
- Fermentation and Sensory Analysis
- Muscle Physiology and Disorders
- Cardiac Imaging and Diagnostics
- Horticultural and Viticultural Research
- Food Quality and Safety Studies
- Genomics and Chromatin Dynamics
- Cardiomyopathy and Myosin Studies
- Radiopharmaceutical Chemistry and Applications
- Medical Imaging Techniques and Applications
- Cardiovascular Function and Risk Factors
- RNA regulation and disease
- Brucella: diagnosis, epidemiology, treatment
- Advanced MRI Techniques and Applications
- Mitochondrial Function and Pathology
- Cell death mechanisms and regulation
- Polyamine Metabolism and Applications
- Viral Infections and Immunology Research
- Biochemical and biochemical processes
- Neurogenetic and Muscular Disorders Research
- Anesthesia and Neurotoxicity Research
- Medical Imaging and Pathology Studies
- Tracheal and airway disorders
- Infrared Thermography in Medicine
Institut de Myologie
2010-2024
Inserm
2015-2024
Centre de Recherche en Myologie
2015-2024
Sorbonne Université
2015-2024
Université de Bordeaux
1995-2017
Centre National de la Recherche Scientifique
2003-2016
Université Paris Cité
2003-2016
Canadian Nautical Research Society
2012-2014
Centre hospitalier universitaire de Québec
2014
Royal Netherlands Academy of Arts and Sciences
2005-2011
The mechanisms underlying cell response to mechanical forces are critical for muscle development and functionality. We aim determine whether mutations of the LMNA gene causing congenital muscular dystrophy impair ability precursors sense tissue stiffness respond challenge. found that LMNA-mutated myoblasts (LMNA) embedded in soft matrix did not align along gel axis whereas control did. were unable tune their cytoskeletal tension as attested by inappropriate cell-matrix adhesion sites versus...
A direct PCR detection test which allows an early of histamine-producing bacteria showed that the presence histidine decarboxylating (HDC<sup>+</sup>) is not rare, as almost half 118 tested wines possessed carrying decarboxylase gene. relationship between amplification a 500 bp fragment, enzymatic activity and amine production was observed. Wines for negative never contained histamine. While those HDC<sup>+</sup> bacteria, always histamine in varying amounts. All strains belonged to...
Apoptosis-inducing factor (AIF), or programmed cell death 8 (Pdcd8), is a highly conserved, ubiquitous flavoprotein localized in the mitochondrial intermembrane space. In vivo, AIF provides protection against neuronal apoptosis induced by oxidative stress. Conversely, vitro, has been demonstrated to have proapoptotic role when, on induction of pathway, translocates nucleus where it facilitates chromatin condensation and large scale DNA fragmentation. To determine myocardial apoptotic...
Three techniques for studying nasal mucociliary clearance have been compared in 20 normal subjects, aged 19 to 26. different tracers used were deposited on the septum or floor. transport rate of a 250 μm anion resin particle tagged with 99mTc was measured over distance 3.5 cm. This method as reference. time saccharin when subjects tasted its sweetness swallowing. 600 aluminium discs by observing their arrival at pharynx. each method, duplicate measurements carried out 24 hour period. Despite...
Selenoprotein N (SelN) deficiency causes a group of inherited neuromuscular disorders termed SEPN1-related myopathies (SEPN1-RM). Although the function SelN remains unknown, recent data demonstrated that it is dispensable for mouse embryogenesis and suggested its involvement in regulation ryanodine receptors and/or cellular redox homeostasis. Here, we investigate role satellite cell (SC) muscle regeneration, using Sepn1−/− model. Following cardiotoxin-induced injury, expression was strongly...
Wines containing high levels of biogenic amines were investigated for the presence tyramine‐producing strains. Two different Lactobacillus brevis (IOEB 9809 and IOEB 9901) able to produce amine isolated. None isolated strains identified as Oenococcus oeni formed tyramine. In addition, other Lact. hilgardii from our collection (IOEB) American Type Culture Collection (ATCC) strong tyramine producers. 9649 found phenylethylamine simultaneously. The conditions that can influence formation in...
Lysozyme was evaluated for its ability to reduce the lactic bacteria flora in musts and wines after completion of malolactic conversion. An addition 500 mg lysozyme per liter grape must inhibited fermentation, while 250 mg/L red wines, promoted microbiological stabilization. Control lots (without lysozyme) had higher bacterial populations. In which added, there no increase content acetic acid biogenic amines during a period six months at 18°C. fermentation processing volatile acidity levels...
Chardonnay grapes from a single vineyard block were held after crushing for 20 to 25 hours at four different temperatures between 9°C and 30°C, pressed, vinified separately but identically. Must samples taken five-hour intervals showed greatly increased phenolic extraction rates higher temperatures, particularly of the flavonoid fraction. Wine results similar, showing content in warm-skin-contact wines. Temperature control below 15°C minimized wine protein levels bentonite requirements....
Serum response factor (SRF) is a crucial transcriptional for muscle-specific gene expression. We investigated SRF function in adult skeletal muscles, using mice with postmitotic myofiber-targeted disruption of the gene. Mutant displayed severe muscle mass reductions due to postnatal growth defect resulting highly hypotrophic myofibers. SRF-depleted myofibers also failed regenerate following injury. Muscles lacking had very low levels creatine kinase and alpha-actin (SKA) transcripts other...
The LMNA gene encodes lamin A/C intermediate filaments that polymerize beneath the nuclear membrane, and are also found in nucleoplasm an uncharacterized assembly state. They thought to have structural functions regulatory roles signaling pathways via interaction with transcription factors. Mutations been involved numerous inherited human diseases, including severe congenital muscular dystrophy (L-CMD). We created Lmna(ΔK32) knock-in mouse harboring a L-CMD mutation. Lmna(ΔK32/ΔK32) mice...
Dilated cardiomyopathy (DCM) associates left ventricular (LV) dilatation and systolic dysfunction is a major cause of heart failure cardiac transplantation. LMNA gene encodes lamins A/C, proteins the nuclear envelope. mutations DCM with conduction and/or rhythm defects. The pathomechanisms linking to remain be elucidated. We investigated phenotype associated heterozygous LmnaΔK32/+ (Het) knock-in mice, which carry human mutation. Het mice developed cardiac-specific phenotype. Two phases, two...
During malolactic fermentation (MLF), the secondary metabolisms of lactic acid bacteria (LAB) contribute to organoleptic modification wine. To understand contribution MLF, we evaluated capacity various wine LAB metabolize methionine.Using gas chromatography (GC) coupled either with mass spectrometry (MS) or a flame photometry detector in sulphur mode (FPD), studied this metabolism laboratory media and In media, several isolated from were able methionine. They formed methanethiol, dimethyl...
Dystonia, cerebellar atrophy, and cardiomyopathy constitute a rare association. We used homozygosity mapping whole exome sequencing to determine the mutation, western blot immunolabelling on cultured fibroblasts demonstrate lower expression mislocalization of protein. report boy born from consanguineous healthy parents, who presented at three years age with rapidly progressing dystonia, progressive dilated cardiomyopathy. identified regions performed that revealed homozygous missense...
Dilated cardiomyopathy (DCM) is characterized by ventricular dilation associated with systolic dysfunction, which could be caused mutations in lamina/C gene (LMNA). LMNA-linked DCM severe males both human patients and a knock-in mouse model carrying homozygous p.H222P mutation (LmnaH222P/H222P). The aim of this study was to investigate the molecular mechanisms underlying gender difference DCM. A whole-exome analysis multiplex family exhibiting revealed DCM-linked LMNA mutation, p.R225X....
LMNA encodes for Lamin A/C, type V intermediate filaments that polymerize under the inner nuclear membrane to form lamina. A small fraction of less polymerized, is also found in nucleoplasm. A/C functions include roles resistance mechanical stress and gene regulation. mutations are responsible a wide variety pathologies, including Emery–Dreifuss (EDMD) LMNA-related congenital muscular dystrophies (L-CMD) without clear genotype–phenotype correlations. Both diseases presented with striated...
The purpose of this work was to study the involvement micro-organisms, which develop together with Botrytis cinerea on grapes, in SO2 binding power musts.Yeasts and bacteria were involved. Most acetic acid bacteria, mainly Gluconobacter genus. Unlike oxidative yeasts, produce gluconic (in balance delta-gluconolactone) from glucose, 5-oxofructose fructose dihydroxyacetone glycerol. Production carbonyl compounds other sugars polyols not detected or very weak.Acetic are responsible for...
Journal Article Vanillin production from simple phenols by wine‐associated lactic acid bacteria Get access A. Bloem, Bloem UMR 1219 INRA/Université Victor Segalen Bordeaux 2, Faculté d'Œnologie, ISVV Talence, France Search for other works this author on: Oxford Academic Google Scholar Bertrand, Bertrand Lonvaud‐Funel, Lonvaud‐Funel G. De Revel Gilles de Revel, France. E‐mail: revel@oenologie.u‐bordeaux2.fr Letters in Applied Microbiology, Volume 44, Issue 1, 1 January 2007, Pages 62–67,...