A5060606926- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Protein Structure and Dynamics
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
Hunan Normal University
2022-2025
Changsha Normal University
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Hunan University
2022-2024
National Institute for Viral Disease Control and Prevention
2024
T5 is a siphophage that has been extensively studied by structural and biochemical methods. However, the complete in situ structures of before after DNA ejection remain unknown. In this study, we used cryo-electron microscopy (cryo-EM) to determine mature (a laboratory-adapted, fiberless mutant) urea-treated empty (lacking tip complex) at near-atomic resolutions. Atomic models head, connector complex, tail tube, were built for T5, atomic comprising portal protein pb7, adaptor p144,...
It is estimated that over 60% of known tailed phages are siphophages, which characterized by a long, flexible, and non-contractile tail. Nevertheless, entire high-resolution structures siphophages remain scarce. Using cryo-EM, we resolved the T-series siphophage T1, encompassing its head, connector complex, tail tube, tip, at near-atomic resolution. The density maps enabled us to build atomic models for majority T1 proteins. head comprises 415 copies major capsid protein gp47, arranged into...
Archaeal viruses with a spindle-shaped virion are abundant and widespread in extremely diverse environments. However, efforts to obtain the high-resolution structure of virus have been unsuccessful. Here, we present SSV19, infecting hyperthermophilic archaeon Sulfolobus sp. E11-6. Our near-atomic reveals an unusual sevenfold symmetrical tail consisting tailspike, nozzle, adaptor proteins. The capsid shell is formed by seven left-handed helical strands, constructed hydrophobic major protein,...