A5031203283- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Viral Infections and Vectors
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- Viral Infections and Outbreaks Research
- RNA and protein synthesis mechanisms
- Viral Infections and Immunology Research
- Vector-Borne Animal Diseases
- Influenza Virus Research Studies
- Genomics and Phylogenetic Studies
- COVID-19 Clinical Research Studies
- Plant Virus Research Studies
- COVID-19 diagnosis using AI
- Viral Infectious Diseases and Gene Expression in Insects
- Bacterial Genetics and Biotechnology
- Parvovirus B19 Infection Studies
- SARS-CoV-2 detection and testing
- Poxvirus research and outbreaks
- Bacillus and Francisella bacterial research
- Herpesvirus Infections and Treatments
- Energy Harvesting in Wireless Networks
National Institute for Viral Disease Control and Prevention
2016-2025
Hunan Normal University
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2007-2025
Changsha Normal University
2025
Peking Union Medical College Hospital
2025
Chinese Center For Disease Control and Prevention
2012-2023
National Health and Family Planning Commission
2013-2022
Xi'an Jiaotong University
2022
Centers for Disease Control and Prevention
2021
Qingdao University
2020
In December 2019, a cluster of patients with pneumonia unknown cause was linked to seafood wholesale market in Wuhan, China. A previously betacoronavirus discovered through the use unbiased sequencing samples from pneumonia. Human airway epithelial cells were used isolate novel coronavirus, named 2019-nCoV, which formed clade within subgenus sarbecovirus, Orthocoronavirinae subfamily. Different both MERS-CoV and SARS-CoV, 2019-nCoV is seventh member family coronaviruses that infect humans....
The novel coronavirus (2019-nCoV) is spreading very fast in Hubei Province of China.As February 14, 2020, 51,986 confirmed cases (including laboratory-confirmed and clinically-confirmed cases) were reported Province, 1,318 them died.Respiratory droplets contact transmission are considered to be the most important routes 2019-nCoV, but do not fully account for occurrence all disease 2019 (COVID-19) cases, previously known as pneumonia (NCP), reasons rapid spread this virus (1).In Biosafety...
Abstract SARS-CoV-2, a β-coronavirus, has rapidly spread across the world, highlighting its high transmissibility, but underlying morphogenesis and pathogenesis remain poorly understood. Here, we characterize replication dynamics, cell tropism of SARS-CoV-2 in organotypic human airway epithelial (HAE) cultures. replicates efficiently infects both ciliated secretory cells HAE In comparison, HCoV-NL63 to lower titers is only detected cells. shows similar morphogenetic process as other...
An outbreak of unusual respiratory disease, initially dominated by pneumonia, in Wuhan, China, is caused infection a novel coronavirus. The new virus was named 2019-nCoV WHO.1Zhu N Zhang D Wang W et al.A coronavirus from patients with pneumonia 2019.N Engl J Med. 2020; (published online Jan 24.)DOI:10.1056/NEJMoa2001017Crossref PubMed Scopus (19264) Google Scholar, 2Zhou P Yang XL XG associated probable bat origin.Nature. Feb 3.)DOI:10.1038/s41586-020-2012-7Crossref (14900) 3Wu F Zhao S Yu B...
In recent years, coxsackievirus A6 (CVA6) has surpassed enterovirus A71 to become the main pathogen causing severe Hand, Foot, and Mouth disease (HFMD) in China with a substantial burden. However, there is currently no commercial CVA6 vaccine. The D3a genotype of predominant China. this study, virus-like particles (VLPs) mRNA vaccines based on sub-genotype were successfully developed. immunogenicity protective effects VLP combined Al(OH)3 CpG adjuvant indicated that VLP-induced neutralizing...
Blocking autophagy reduces the extent of lung damage and decreases mortality in mice infected with H5N1 strain avian influenza.
Cryo-electron microscopy is an essential tool for high-resolution structural studies of biological systems. This method relies on the use phase contrast imaging at high defocus to improve information transfer low spatial frequencies expense higher frequencies. Here we demonstrate that electron ptychography can recover specimen with continuous across a wide range frequency spectrum, improved lower frequencies, and as such more efficient recovery than conventional imaging. We further show be...
The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) in 2002 and Middle East (MERS-CoV) 2012 has generated enormous interest the biodiversity, genomics cross-species transmission potential coronaviruses, especially those from bats, second most speciose order mammals. Herein, we identified a novel coronavirus, provisionally designated Rousettus bat GCCDC1 (Ro-BatCoV GCCDC1), rectal swab samples leschenaulti bats by using pan-coronavirus RT-PCR next-generation sequencing....
Abstract The membrane-associated RING-CH (MARCH) proteins are E3 ligases that regulate the stability of various cellular membrane proteins. MARCH8 has been reported to inhibit infection HIV-1 and a few other viruses, thus plays an important role in host antiviral defense. However, spectrum underlying mechanisms incompletely defined. Here, we demonstrate profoundly inhibits influenza A virus (IAV) replication both vitro mice. Mechanistically, suppresses IAV release through redirecting viral...
Abstract Emerging in December 2019, coronavirus disease 2019 (COVID-19) eventually became a pandemic and has posed tremendous threat to global public health. However, the origins of SARS-CoV-2, causative agent COVID-19, remain be determined. It reported that certain number early case clusters had contact history with Huanan Seafood Market. Therefore, surveillance SARS-CoV-2 within market is vital importance. Herein, we presented detection results 1380 samples collected from environment...
ABSTRACT Myophage Mu is a representative of contractile nanomachines with simple tail baseplate. It has the capacity to infect range intestinal bacteria and extensive applications in genetic engineering research. Nevertheless, comprehensive understanding entire structure mechanisms remains elusive. Using cryo-electron microscopy (cryo-EM), we resolved asymmetric structures both its extended contracted states, latter which lacked baseplate, at near-atomic resolutions. We built atomic models...
Coronavirus disease 2019 (COVID-19) has become a pandemic with increasing numbers of cases worldwide. SARS-CoV-2, the causative virus COVID-19, is mainly transmitted through respiratory droplets or direct and indirect contact an infected person. The possibility potential faecal-oral transmission was investigated in this study. We collected 258 faecal specimens from nine provinces China detected nucleic acid SARS-CoV-2 using real-time RT–PCR. Vero cells were used to isolate positive samples,...
Significance Many double-stranded DNA (dsDNA) viruses, including the tailed dsDNA bacteriophages and herpesviruses, use molecular machines at a unique vertex of capsid for genome delivery. Bacteriophage T7 provides tractable model study viruses. Here, we report structure mature bacteriophage T7, as well structures full empty particles in complex with their cell receptor lipopolysaccharide. The symmetry-mismatch vertex, internal ejection proteins, have been resolved near-atomic resolutions...
<abstract abstract-type="Abstract"> <sec> <b>Introduction</b> The first imported case of monkeypox (MPX) from the mainland China was reported in September 2022. Herein, study reports isolation and characterization MPX virus (MPXV) this case. </sec><sec> <b>Methods</b> Clinical specimens including skin blister fluid, oropharyngeal nasopharyngeal swabs, blood were collected inoculated onto Vero cells. isolated identified as MPXV using quantitative polymerase chain reaction (qPCR), cytopathic...
Siphophages have a long, flexible, and noncontractile tail that connects to the capsid through neck. The phage is essential for host cell recognition virus-host interactions; moreover, it serves as channel genome delivery during infection. However, in situ high-resolution structure of neck-tail complex siphophages remains unknown. Here, we present siphophage lambda "wild type," most widely used, laboratory-adapted fiberless mutant. comprises formed by stacked 12-fold hexameric rings 3-fold...
The formation of many double-stranded DNA viruses, such as herpesviruses and bacteriophages, begins with the scaffolding-protein-mediated assembly procapsid. Subsequently, procapsid undergoes extensive structural rearrangement expansion to become mature capsid. Bacteriophage P22 is an established model system used study virus maturation. Here, we report cryo-electron microscopy structures procapsid, empty capsid, capsid phage at resolutions 2.6 Å, 3.9 2.8 3.0 respectively. structure allowed...
T5 is a siphophage that has been extensively studied by structural and biochemical methods. However, the complete in situ structures of before after DNA ejection remain unknown. In this study, we used cryo-electron microscopy (cryo-EM) to determine mature (a laboratory-adapted, fiberless mutant) urea-treated empty (lacking tip complex) at near-atomic resolutions. Atomic models head, connector complex, tail tube, were built for T5, atomic comprising portal protein pb7, adaptor p144,...
Background Mammalian orthoreoviruses (MRVs) have a wide geographic distribution and can infect virtually all mammals. Infections in humans may be either symptomatic or asymptomatic. This study describes the isolation identification of natural reassortant MRV from least horseshoe bats (Rhinolophus pusillu) China, referred to as RpMRV-YN2012. Methods Results The RpMRV-YN2012 was obtained urine samples Rhinolophus pusillus by cell culture. Negative-staining electron microscopy revealed that...