A5102018492- Influenza Virus Research Studies
- Respiratory viral infections research
- Viral Infections and Vectors
- interferon and immune responses
- Animal Disease Management and Epidemiology
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- COVID-19 epidemiological studies
- COVID-19 Clinical Research Studies
- Immune Response and Inflammation
- Viral Infections and Outbreaks Research
- vaccines and immunoinformatics approaches
- Bacteriophages and microbial interactions
- Diabetes and associated disorders
- Virus-based gene therapy research
- Mosquito-borne diseases and control
- Vaccine Coverage and Hesitancy
- Hepatitis B Virus Studies
- Gut microbiota and health
- Monoclonal and Polyclonal Antibodies Research
- Virology and Viral Diseases
- Herpesvirus Infections and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Virus Infections Studies
- Pneumonia and Respiratory Infections
Sun Yat-sen University
2018-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2021-2025
Peking Union Medical College Hospital
2021-2023
Academy of Medical Sciences
2023
National Institute for Viral Disease Control and Prevention
2010-2021
Ministry of Education of the People's Republic of China
2021
National Health and Family Planning Commission
2019
Chinese Center For Disease Control and Prevention
2007-2018
Shenzhen Third People’s Hospital
2014
University of Hong Kong
2014
The first identified cases of avian influenza A(H7N9) virus infection in humans occurred China during February and March 2013. We analyzed data obtained from field investigations to describe the epidemiologic characteristics H7N9 as December 1, 2013.Field were conducted for each confirmed case infection. A patient was considered have a if presence verified by means real-time reverse-transcriptase-polymerase-chain-reaction assay (RT-PCR), viral isolation, or serologic testing. Information on...
An outbreak of unusual respiratory disease, initially dominated by pneumonia, in Wuhan, China, is caused infection a novel coronavirus. The new virus was named 2019-nCoV WHO.1Zhu N Zhang D Wang W et al.A coronavirus from patients with pneumonia 2019.N Engl J Med. 2020; (published online Jan 24.)DOI:10.1056/NEJMoa2001017Crossref PubMed Scopus (19264) Google Scholar, 2Zhou P Yang XL XG associated probable bat origin.Nature. Feb 3.)DOI:10.1038/s41586-020-2012-7Crossref (14900) 3Wu F Zhao S Yu B...
Pathological studies on fatal cases caused by 2009 pandemic influenza H1N1 virus (2009 pH1N1) reported extensive diffuse alveolar damage and infection predominantly in the lung parenchyma. However, host immune response after severe pH1N1 is poorly understood. Herein, we investigated viral load, response, apoptosis tissues from 50 with infection. The results suggested that 7 of 27 cytokines/chemokines showed remarkably high expression, including IL-1 receptor antagonist protein, IL-6, tumor...
Zoonotic avian influenza A virus (IAV) infections are rare. Sustained transmission of these IAVs between humans has not been observed, suggesting a role for host genes. We used whole-genome sequencing to compare IAV H7N9 patients with healthy controls and observed strong association infection rare, heterozygous single-nucleotide variants in the MX1 gene. codes myxovirus resistance protein (MxA), an interferon-induced antiviral guanosine triphosphatase known control transgenic mice. Most MxA...
In conventional attenuated viral vaccines, immunogenicity is often suboptimal. Here we present a systematic approach for vaccine development that eliminates interferon (IFN)-modulating functions genome-wide while maintaining virus replication fitness. We applied quantitative high-throughput genomics system to influenza A simultaneously measured the fitness and IFN sensitivity of mutations across entire genome. By incorporating eight IFN-sensitive mutations, generated...
Current metagenomic tools can fail to identify highly divergent RNA viruses. We developed a deep learning algorithm, termed LucaProt, discover RNA-dependent polymerase (RdRP) sequences in 10,487 metatranscriptomes generated from diverse global ecosystems. LucaProt integrates both sequence and predicted structural information, enabling the accurate detection of RdRP sequences. Using this approach we identified 161,979 potential virus species 180 supergroups, including many previously poorly...
The development of a universal influenza vaccine to elicit broad immune responses is essential in reducing disease burden and pandemic impact. In this study, the mosaic design strategy genetic algorithms were utilized optimize seasonal A virus (H1N1, H3N2) hemagglutinin (HA) neuraminidase (NA) antigens, which also contain most potential T-cell epitopes. These immunogens then expressed as virus-like particles (VLPs) using baculovirus expression system. immunogenicity protection effectiveness...
Most existing vaccines, delivered by intramuscular injection (IM), are typically associated with stringent storage requirements under cold-chain distribution and professional administration medical personnel often result in the induction of weak mucosal immunity. In this context, we reported a microneedle (MN) patch to deliver chitosan oligosaccharide (COS)-encapsulated DNA vaccines (DNA@COS) encoding spike nucleocapsid proteins SARS-CoV-2 as vaccination technology. Compared IM immunization,...
Developing broad-spectrum influenza vaccines is crucial for control and potential pandemic preparedness. Here, we reported a novel vaccine design utilizing circular RNA (circRNA) as delivery platform multi-subtype neuraminidases (NA) (influenza A N1, N2, B Victoria lineage NA) immunogens. Individual NA circRNA lipid nanoparticles (LNP) elicited robust NA-specific antibody responses with neuraminidase inhibition activity (NAI), preventing the virus from egressing infecting neighboring cells....
Abstract We investigated potential sources of infection for 6 confirmed influenza A (H5N1) patients who resided in urban areas People’s Republic China. None had known exposure to sick poultry or that died from illness, but all visited wet markets before illness.
In spring 2013, influenza A(H7N9) virus was isolated from an apparently healthy tree sparrow in Chongming Dongping National Forest Park, Shanghai City, China. The entire gene constellation of the is similar to that isolates humans, highlighting need monitor viruses different species.
Tryptophan (Trp) metabolism through the kynurenine pathway (KP) is well known to play a critical function in cancer, autoimmune and neurodegenerative diseases. However, its role host-pathogen interactions has not been characterized yet. Herein, we identified that kynurenine-3-monooxygenase (KMO), key rate-limiting enzyme KP, quinolinic acid (QUIN), enzymatic product of KMO enzyme, exerted novel antiviral against broad range viruses. Mechanistically, QUIN induced production type I interferon...
On May 7, 2009, a national network was organized in the People's Republic of China for surveillance, reporting, diagnosis, and treatment influenza A pandemic (H1N1) 2009 virus infection (pandemic [H1N1] 2009). Persons with suspected cases are required to report Chinese Center Disease Control Prevention Ministry Health within 24 hours; patient's close contacts then traced placed quarantine 7 days. We 3 confirmed early 2009. Two were imported from United States; other Canada. The patients...
The haemagglutinin (HA) glycoprotein of influenza A virus is a major antigen that initiates humoral immunity against infection; however, the cellular immune response HA poorly understood. Furthermore, HA-derived cytotoxic T-lymphocyte (CTL) epitopes are relatively rare in comparison to other internal gene products. Here, CTL serotype H5 protein were screened. By using silico prediction, vitro refolding and T2 cell-binding assay, followed by immunization HLA-A2.1/Kb transgenic mice, an...
Avian-origin influenza A(H7N9) recently emerged in China, causing severe human disease. Several subtype H7N9 isolates contain genes previously identified viruses from finch-like birds. Because wild and domestic songbirds interact with humans poultry, we investigated the susceptibility transmissibility of these species. Finches, sparrows, parakeets supported replication a isolate, shed high titers through oropharyngeal route, showed few disease signs. Virus was into water troughs, several...
Abstract The H7N9 influenza virus poses a significant threat to human health, and the mechanism by which it infects humans remains incompletely understood. Our investigation has unveiled insights into role of glucosidase alpha, neutral C ( GANC ) gene in infections. Through whole genome sequencing (WGS), we identified five low-frequency functional heterozygous variants strongly associated with infections compared healthy controls. Furthermore, observed reduction mRNA protein expression...
<title>Abstract</title> Background Residential greenness, a fundamental component of urban design, could contribute to the prevention respiratory infections via several potential mechanisms.. However, health benefits greenness on influenza epidemics in real world are not as clear. Therefore, present study, we investigated association between residential exposure and virus infection risk using large diverse cross-sectional dataset from Chinese surveillance system. Methods In this...
The emergence of severe acute respiratory syndrome (SARS) underscored the importance influenza detection and response in China. From 2004, Chinese National Influenza Center (CNIC) United States Centers for Disease Control Prevention (USCDC) initiated Cooperative Agreements to build capacity surveillance 2004 2014, CNIC USCDC collaborated on following activities: 1) developing human technical expertise virology epidemiology China; 2) a comprehensive system by enhancing influenza-like illness...
ABSTRACT Companion animals such as cats and dogs harbor diverse microbial communities that can potentially impact human health due to close frequent contact. To better characterize their total infectomes assess zoonotic risks, we characterized the overall of companion (cats dogs) evaluated potential risks. Meta-transcriptomic analyses were performed on 239 samples from collected across China, identifying 24 viral species, 270 bacterial genera, two fungal genera. Differences in microbiome...